The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Osteocalcin Mediates Biomineralization during Osteogenic Maturation in Human Mesenchymal Stromal Cells
Open accessAbstract
There is a growing interest in cell therapies using mesenchymal stromal cells (MSCs) for repairing bone defects. MSCs have the ability to differentiate into osteoprogenitors and osteoblasts as well as to form calcified bone matrix. However, the molecular mechanisms governing mineralization during osteogenic differentiation remain unclear. Non-collagenous proteins in the extracellular matrix are believed to control different aspects of the mineralization. Since osteocalcin is the most abundant non-collagenous bone matrix protein, the purpose of this study is to investigate the roles of osteocalcin in mineral species production during osteogenesis of MSCs. Using Raman spectroscopy, we found that the maturation of mineral species was affected by osteocalcin expression level. After osteocalcin was knocked down, the mineral species maturation was delayed and total hydroxyapatite was lower than the control group. In addition, the expression of osteogenic marker genes, including RUNX2, alkaline phosphatase, type I collagen, and osteonectin, was downregulated during osteogenic differentiation compared to the control group; whereas gene expression of osterix was upregulated after the knockdown. Together, osteocalcin plays an essential role for the maturation of mineral species and modulates osteogenic differentiation of MSCs. The results offer new insights into the enhancement of new bone formation, such as for the treatments of osteoporosis and fracture healing.
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Cites
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Cited by
The Development of Molecular Biology of Osteoporosis
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Glucose Metabolism in Osteoblasts in Healthy and Pathophysiological Conditions
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Exosomes to exosome-functionalized scaffolds: a novel approach to stimulate bone regeneration
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Metadata
- Authors
- Tsao, Y.-T., Huang, Y.-J., Wu, H.-H., Liu, Y.-A., Liu, Y.-S., & Lee, O.
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2017-01-17
- Volume
- 18
- Issue
- 1
- Pages
- 159
- ISSN
- 1422-0067 (ISSN-L)
- OpenAlex
- W2578851460
- Cited by
- 265
- References
- 32
- FWCI
- 8.85
- Type
- journal article
- Language
- en
- Open access
- gold
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- Tsao, Y.-T., Huang, Y.-J., Wu, H.-H., Liu, Y.-A., Liu, Y.-S., & Lee, O. (2017). Osteocalcin Mediates Biomineralization during Osteogenic Maturation in Human Mesenchymal Stromal Cells. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms18010159
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