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Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion

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Hewelt-Belka, Weronika, Kot-Wasik, Ágata, Tamagnini, Paula, Oliveira, Paulo·2020·International Journal of Molecular Sciences
DOI 10.3390/ijms21238883PMC PMC7727718
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Abstract

Cyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids’ composition and variation, and the assessment of the lipids’ functional and structural roles have been largely overlooked. Here, we aimed at expanding the cyanobacterial lipidomic analytical pipeline by using an untargeted lipidomics approach. Thus, the lipid composition variation of the model cyanobacterium Synechocystis sp. PCC 6803 was investigated in response to both alternative cultivation setups and gene deletion. This approach allowed for detecting differences in total lipid content, alterations in fatty-acid unsaturation level, and adjustments of specific lipid species among the identified lipid classes. The employed method also revealed that the cultivation setup tested in this work induced a deeper alteration of the cyanobacterial cell lipidome than the deletion of a gene that results in a dramatic increase in the release of lipid-rich outer membrane vesicles. This study further highlights how growth conditions must be carefully selected when cyanobacteria are to be engineered and/or scaled-up for lipid or fatty acids production.

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Cited by

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Authors
Hewelt-Belka, W., Kot-Wasik, Á., Tamagnini, P., & Oliveira, P.
Affiliations
  • Gdańsk University of Technology (PL)
  • Department of Analytical Chemistry, Faculty of Chemistry, Gdańsk University of Technology, Gabriela Narutowicza 11/12, 80-233 Gdańsk, Poland
  • Universidade do Porto (PT)
Corresponding author
Oliveira, Paulo
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2020-11-24
Volume
21
Issue
23
Pages
8883
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms21238883
PMC
PMC7727718
OpenAlex
W3108514525
Cited by
20
References
5
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 4 · 2021: 3
FWCI
1.18
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • FCT - Fundação para a Ciência e a Tecnologia (IF/00256/2015)
  • Fundo Europeu de Desenvolvimento Regional (FEDER) funds through the COMPETE 2020 Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through Fundação para a Ciência e a Tecnologia/Ministério da (POCI-01-0145-FEDER-029540 (PTDC/BIA-OUT/29540/2017))
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Synechocystis sp. PCC 6803, lipidome, untargeted lipidomics, growth conditions, gene deletion, outer membrane vesicles, fatty acid unsaturation, lipid composition, lipid production
MeSH terms
Lipidomics, Cyanobacteria, Fatty Acids, Lipids, Membrane Lipids, Photosynthesis, Gene Expression Regulation, Bacterial, Gene Deletion, Metabolic Engineering

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