Evaluation of methods to extract and quantify lipids from Synechocystis PCC 6803
Abstract
In order to use the photosynthetic cyanobacterium Synechocystis as a source of feedstock for carbon-neutral, nonpetroleum-based diesel fuel, we evaluated several solvents and solvent systems for their ability to extract lipid from wild-type Synechocystis PCC 6803. Chloroform+methanol-based Folch and Bligh & Dyer methods had the highest lipid recoveries. Less toxic solvents, such as methanol and MTBE, or direct trans-esterification of biomass (without pre-extraction step) gave only slightly lower lipid-extraction yields. Ethanol, isopropanol, butanol, hexane, acetic ester, and their combinations were not effective for lipid extraction from Synechocystis (>20% loss), even though they are widely used for non-polar lipid extraction from other feedstock, including algae. We confirmed the success of chloroform+methanol-based extraction by their penetration of the cell membrane system, higher polarity, and stronger interaction with hydrogen bonds. The less-polar solvents not only had lower lipid yield, but also extracted more non-lipid compounds that require extra purification to remove. We also characterized the fatty-acid profile of Synechocystis PCC 6803: C16:0 (∼60%), C16:1 (∼9.5%), C18:0 (∼1.2%), C18:1 (∼2%), C18:2 (∼9.8%), and C18:3 (∼16.5%).
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Unlike the abundant diacylglycerol found in cyanobacteria, TAG constitutes the primary lipid component in green algae.
“Unlike the abundant diacylglycerol (DG) found in cyanobacteria, TAG constitutes the primary lipid component in green algae [39, 40].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [39]
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“Unlike the abundant diacylglycerol (DG) found in cyanobacteria, TAG constitutes the primary lipid component in green algae [39, 40].”
BackgroundSheng, J., Vannela, R., & Rittmann, B. E. (2011) · Environmental Science & Technology · doi:10.1021/es103339x · Cited by 144 · see citing works
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- Authors
- Sheng, J., Vannela, R., & Rittmann, B. E.
- Affiliations
- Arizona State University (US)
- Corresponding author
- Sheng, Jie
- ORCID
- Rittmann, Bruce E.
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2011-01
- Volume
- 102
- Issue
- 2
- Pages
- 1697-1703
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1981460928
- Cited by
- 184
- References
- 4
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 10 · 2023: 11 · 2022: 7 · 2021: 13 · 2020: 17 · 2019: 24 · 2018: 12 · 2017: 9 · 2016: 18 · 2015: 19 · 2014: 18 · 2013: 4 · 2012: 10
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Arizona State University
- School of Life Sciences, Arizona State University
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Synechocystis sp. PCC 6803, lipid extraction, Folch method, Bligh and Dyer method, solvent selection, fatty acid composition, biodiesel feedstock, transesterification, nonpolar solvents
- MeSH terms
- Biotechnology, Chromatography, Gas, Chromatography, Thin Layer, Diglycerides, Esters, Fatty Acids, Lipids, Solubility, Solvents, Triglycerides, Biomass, Synechocystis
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