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Cited by 21Extracellular vesicles in diseaseNanoparticle-Based Drug DeliveryCaveolin-1 and cellular processes

Secreted Extracellular Vesicle Molecular Cargo as a Novel Liquid Biopsy Diagnostics of Central Nervous System Diseases

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Monteiro-Reis, Sara, Carvalho-Maia, Carina, Bart, Genevieve, Vainio, Seppo J., Pedro, Juliana, Silva, Eunice R., Sales, Goreti, Henrique, Rui, Jerónimo, Carmen·2021·International Journal of Molecular Sciences
DOI 10.3390/ijms22063267PMC PMC8004928
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Abstract

Secreted extracellular vesicles (EVs) are heterogeneous cell-derived membranous granules which carry a large diversity of molecules and participate in intercellular communication by transferring these molecules to target cells by endocytosis. In the last decade, EVs’ role in several pathological conditions, from etiology to disease progression or therapy evasion, has been consolidated, including in central nervous system (CNS)-related disorders. For this review, we performed a systematic search of original works published, reporting the presence of molecular components expressed in the CNS via EVs, which have been purified from plasma, serum or cerebrospinal fluid. Our aim is to provide a list of molecular EV components that have been identified from both nonpathological conditions and the most common CNS-related disorders. We discuss the methods used to isolate and enrich EVs from specific CNS-cells and the relevance of its components in each disease context.

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  • Liquid biopsy as a minimally invasive alternative to traditional imaging and behavioral assessments facilitates ongoing monitoring of biochemical and molecular alterations in the CNS.

    “The utilization of liquid biopsy as a minimally invasive alternative to traditional imaging and behavioral assessments facilitates the ongoing monitoring of biochemical and molecular alterations in the CNS [181–183].”

    From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [181]

    The Methods and Factors for Isolation section says CNS-derived extracellular vesicle cargo can be analyzed for disease monitoring. Future Perspectives and Conclusions describes patient follow-up through circulating biomarkers as a prospective clinical use, not an established ongoing practice. The paper discusses limitations of imaging biomarkers but does not establish liquid biopsy as an alternative to behavioral assessments. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

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References 120 · Cited by 21

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Authors
Monteiro-Reis, S., Carvalho-Maia, C., Bart, G., Vainio, S. J., Pedro, J., Silva, E. R., Sales, G., Henrique, R., & Jerónimo, C.
Affiliations
  • Cancer Biology & Epigenetics Group, Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), R. Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal
  • Disease Networks Research Unit, Laboratory of Developmental Biology, Faculty of Biochemistry and Molecular Medicine, Infotech Oulu, Kvantum Institute, University of Oulu, 90570 Oulu, Finland
  • Infotech Oulu (FI)
  • IPO Porto (PT)
  • BioMark Sensor Research/Centre of Biological Engineering of Minho University, Campus de Gualtar, 4710-057 Braga, Portugal
  • Universidade do Porto (PT)
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2021-03-23
Volume
22
Issue
6
Pages
3267
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms22063267
PMC
PMC8004928
OpenAlex
W3139030108
Cited by
21
References
120
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 5 · 2021: 4
FWCI
0.50
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Estudo Geral (Universidad de Coimbra) (submittedVersion, cc-by)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by)
Funders
Horizon 2020 Framework Programme (MindGaP-H2020-FETOPEN-2018-2020)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
Keywords
extracellular vesicles, liquid biopsy, central nervous system disorders, cerebrospinal fluid, plasma biomarkers, intercellular communication, neurodegenerative diseases, neuroinflammation
MeSH terms
Extracellular Vesicles, Liquid Biopsy, Central Nervous System Diseases, Chemical Fractionation, Humans, Biomarkers, RNA, Untranslated, Molecular Diagnostic Techniques

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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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