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Cited by 15Plant tissue culture and regenerationBiochemical and biochemical processesPlant biochemistry and biosynthesis

Effect of Sucrose Concentration on Rhaponticum carthamoides (Willd.) Iljin Transformed Root Biomass, Caffeoylquinic Acid Derivative, and Flavonoid Production

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Skała, Ewa, Olszewska, Monika Anna, Makowczyńska, Joanna, Kicel, Agnieszka·2022·International Journal of Molecular Sciences
DOI 10.3390/ijms232213848PMC PMC9693310
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Abstract

Rhaponticum carthamoides (Willd.) Iljin is a rare, pharmacopoeial, and medicinal plant, endemic to Siberia and endangered due to the massive collection of raw material from the natural habitat. The aim of the current study was to estimate the effect of sucrose concentration (0–7%) on R. carthamoides transformed root growth and on caffeoylquinic acid derivative (CQA) and flavonoid production. Sucrose in higher concentrations may induce osmotic stress and thus may affect secondary metabolism in plants. It was revealed that sucrose concentration influenced R. carthamoides transformed root biomass and modified the phenolic compound metabolic pathway. However, the dynamics of both processes varied significantly. The optimal sucrose level was different for biomass accumulation and the biosynthesis of specialized metabolite. The highest dry weight of roots was achieved for 7% sucrose (31.17 g L−1 of dry weight), while 1% sucrose was found to be optimal for phenolic acid and flavonoid production. Considering the dry weight increase and metabolite accumulation, 3% sucrose was revealed to give optimal yields of CQAs (511.1 mg L−1) and flavonoids (38.9 mg L−1). Chlorogenic acid, 3,5-, 4,5-di-O-caffeoylquinic acids, 1,4,5-O-tricaffeoylquinic acid, and a tentatively-identified tricaffeoylquinic acid derivative 1 were found to be the most abundant specialized metabolites among the identified CQAs. Our findings indicate that R. carthamoides transformed roots may be an efficient source of CQA derivatives, with valuable health-promoting activities.

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Authors
Skała, E., Olszewska, M. A., Makowczyńska, J., & Kicel, A.
Affiliations
Medical University of Lodz (PL)
Corresponding author
Skała, Ewa
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2022-11-10
Volume
23
Issue
22
Pages
13848
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms232213848
PMC
PMC9693310
OpenAlex
W4308697951
Cited by
15
References
65
Citations by year
2026: 1 · 2025: 9 · 2024: 4 · 2023: 1
FWCI
1.14
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Medical University of Lodz (503/3-012-01/503-31-001-19-00, 503/3-022-01/503-31-001-19-00)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Rhaponticum carthamoides, sucrose concentration, transformed roots, caffeoylquinic acids, flavonoids, chlorogenic acid, phenolic acids, secondary metabolism, osmotic stress, root biomass, medicinal plant cultivation, endangered medicinal plants
MeSH terms
Body Weight, Chlorogenic Acid, Flavonoids, Quinic Acid, Sucrose, Biomass, Leuzea

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