Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production
Open accessAbstract
The organogenic competence of different explants of Rhaponticum carthamoides was investigated on MS agar medium supplemented with BA, IBA or NAA at concentrations of 0.2 and 0.5 mg L −1 . Adventitious shoot formation was obtained through direct organogenesis using leaves of in vitro cultures as explants and through indirect organogenesis when seedling explants (hypocotyl, cotyledon and root) were used for regenerative callus initiation. The shoots were rooted on half-strength MS medium (½ MS) without auxin or containing IBA (0.2–2.0 mg L −1 ). The plantlets regenerated through direct and indirect organogenesis were transferred into pots and grown in the greenhouse for 3 months. Significant differences in morphology, accumulation of chlorogenic acid and 20-hydroxyecdysone (20-HE) as well as in genetic profile were observed between these plants. UHPLC analysis showed that the highest level of chlorogenic acid (12 mg g −1 DW) was found in leaves of plants developed directly from explants, whereas leaves of plants developed via callus tissue accumulated the highest amount of 20-HE (7.4 mg g −1 DW). Its level exceeded that detected in leaves of 3-month-old plants obtained from seeds (2.4 mg g −1 DW). Genetic variations of R. carthamoides regenerated plants were evaluated by flow cytometry and RAPD and ISSR methods. Flow cytometry confirmed similar ploidy level in the mother plant and plants regenerated through direct and indirect organogenesis. Genetic similarity values calculated on the basis of RAPD and ISSR data among regenerated in vitro plants to the mother plant were ranged from 0.765 to 0.941 and 0.647 to 0.947, respectively.
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- Authors
- Skała, E., Grąbkowska, R., Sitarek, P., Kuźma, Ł., Błauż, A., & Wysokińska, H.
- Affiliations
- Medical University of Lodz (PL)
- University of Łódź (PL)
- Corresponding author
- Skała, Ewa
- Venue
- Plant Cell Tissue and Organ Culture (PCTOC)
- Published
- 2015-07-07
- Volume
- 123
- Issue
- 1
- Pages
- 83-98
- ISSN
- 0167-6857 (ISSN-L)
- 1573-5044
- OpenAlex
- W1544536169
- Cited by
- 38
- References
- 39
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 6 · 2023: 1 · 2022: 5 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 2
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Plant Cell Tissue and Organ Culture (PCTOC) (publishedVersion, cc-by)
- Biotechnologia (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Rhaponticum carthamoides, in vitro regeneration, direct organogenesis, indirect organogenesis, adventitious shoot formation, callus induction, chlorogenic acid, 20-hydroxyecdysone, secondary metabolites, genetic variation, ploidy level, RAPD markers, ISSR markers, plant growth regulators
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