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Cited by 19Digital Holography and MicroscopyOptical measurement and interference techniquesOrbital Angular Momentum in Optics

Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy

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Romolo, Anna, Jan, Zala, Bedina Zavec, Apolonija, Kisovec, Matic, Arrigler, Vesna, Spasovski, Vesna, Podobnik, Marjetka, Iglič, Aleš +3 more·2022·International Journal of Molecular Sciences
DOI 10.3390/ijms232415801PMC PMC9779747
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Abstract

Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated.

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  • The Videodrop system detects nanoparticles in solution by analyzing the interference pattern generated by light scattered by each particle, enabling real-time tracking of their Brownian motion.

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Authors
Romolo, A., Jan, Z., Bedina Zavec, A., Kisovec, M., Arrigler, V., Spasovski, V., Podobnik, M., Iglič, A., Pocsfalvi, G., Kogej, K., & Kralj-Iglič, V.
Affiliations
  • University of Ljubljana (SI)
  • Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubjana, Slovenia
  • National Institute of Chemistry (SI)
  • Extracellular Vesicles and Mass Spectrometry Group, Institute of Biosciences and Bioresources, National Research Council of Italy, 80131 Naples, Italy
  • University of Ljubljana, Faculty of Health Sciences, Laboratory of Clinical Biophysics, SI-1000 Ljubljana, Slovenia
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2022-12-13
Volume
23
Issue
24
Pages
15801
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms232415801
PMC
PMC9779747
OpenAlex
W4312139919
Cited by
19
References
66
Citations by year
2026: 4 · 2025: 3 · 2024: 5 · 2023: 6
FWCI
1.44
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Repository of the University of Ljubljana (University of Ljubljana) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • IMAGINE - Repository of the Institute of molecular genetics and genetic engineering (University of Belgrade) (submittedVersion, cc-by)
Funders
Slovenian Research Agency (P2-0232, P3-0388, P1-0201, J3-3066, L3-2621, IO-0003)
Topics
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Digital Holography and Microscopy
  • Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Optical measurement and interference techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Orbital Angular Momentum in Optics
Keywords
hydrodynamic radius, particle number density, liposomes, erythrocytes, plasma, cryo-TEM, thin filament, cell-cell interactions
MeSH terms
Dynamic Light Scattering, Liposomes, Particle Size, Culture Media, Conditioned, Cryoelectron Microscopy, Microscopy, Electron, Transmission

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