Cited by 1,885Field-Flow Fractionation TechniquesNanoparticle-Based Drug DeliveryAnalytical Methods in Pharmaceuticals

Critical evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates

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Filipe, Vasco, Hawe, Andrea, Jiskoot, Wim·2010·Pharmaceutical Research

Abstract

PURPOSE: To evaluate the nanoparticle tracking analysis (NTA) technique, compare it with dynamic light scattering (DLS) and test its performance in characterizing drug delivery nanoparticles and protein aggregates. METHODS: Standard polystyrene beads of sizes ranging from 60 to 1,000 nm and physical mixtures thereof were analyzed with NTA and DLS. The influence of different ratios of particle populations was tested. Drug delivery nanoparticles and protein aggregates were analyzed by NTA and DLS. Live monitoring of heat-induced protein aggregation was performed with NTA. RESULTS: NTA was shown to accurately analyze the size distribution of monodisperse and polydisperse samples. Sample visualization and individual particle tracking are features that enable a thorough size distribution analysis. The presence of small amounts of large (1,000 nm) particles generally does not compromise the accuracy of NTA measurements, and a broad range of population ratios can easily be detected and accurately sized. NTA proved to be suitable to characterize drug delivery nanoparticles and protein aggregates, complementing DLS. Live monitoring of heat-induced protein aggregation provides information about aggregation kinetics and size of submicron aggregates. CONCLUSION: NTA is a powerful characterization technique that complements DLS and is particularly valuable for analyzing polydisperse nanosized particles and protein aggregates.

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Authors
Filipe, V., Hawe, A., & Jiskoot, W.
Affiliations
Leiden University (NL)
Corresponding author
Jiskoot, Wim
Venue
Pharmaceutical Research
Published
2010-03-04
Volume
27
Issue
5
Pages
796-810
ISSN
  • 0724-8741 (ISSN-L)
  • 1573-904X
OpenAlex
W2131083445
Cited by
1,885
References
20
Citations by year
2026: 112 · 2025: 149 · 2024: 127 · 2023: 132 · 2022: 123 · 2021: 172 · 2020: 138 · 2019: 143 · 2018: 134 · 2017: 112 · 2016: 152 · 2015: 109 · 2014: 87 · 2013: 75 · 2012: 72
FWCI
60.48
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Pharmaceutical Research (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Computational Mechanics › Field-Flow Fractionation Techniques
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
Keywords
nanoparticle tracking analysis, dynamic light scattering, nanoparticle size distribution, protein aggregates, drug delivery nanoparticles, heat-induced protein aggregation, aggregation kinetics, polydisperse particles, polystyrene microspheres
MeSH terms
Algorithms, Excipients, Immunoglobulin G, Light, Particle Size, Polystyrenes, Proteins, Scattering, Radiation, Software, Viscosity, Drug Delivery Systems, Nanotechnology, Nanoparticles

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