Genistein and Procyanidin B2 Reduce Carcinogen-Induced Reactive Oxygen Species and DNA Damage through the Activation of Nrf2/ARE Cell Signaling in Bronchial Epithelial Cells In Vitro

Suraweera, Tharindu L.; Merlin, J. P. Jose; Dellaire, Graham; Xu, Zhaolin; Rupasinghe, H. P. Vasantha

doi:10.3390/ijms24043676

Cited by 35Genomics, phytochemicals, and oxidative stressPhytochemicals and Antioxidant ActivitiesBioactive Compounds and Antitumor Agents

Genistein and Procyanidin B2 Reduce Carcinogen-Induced Reactive Oxygen Species and DNA Damage through the Activation of Nrf2/ARE Cell Signaling in Bronchial Epithelial Cells In Vitro

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Suraweera, Tharindu L., Merlin, J. P. Jose, Dellaire, Graham, Xu, Zhaolin, Rupasinghe, H. P. Vasantha·2023·International Journal of Molecular Sciences

DOI 10.3390/ijms24043676PMC PMC9961944

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Abstract

Cancer is one of the leading causes of death worldwide. Chemotherapy and radiation therapy are currently providing the basis for cancer therapies, although both are associated with significant side effects. Thus, cancer prevention through dietary modifications has been receiving growing interest. The potential of selected flavonoids in reducing carcinogen-induced reactive oxygen species (ROS) and DNA damage through the activation of nuclear factor erythroid 2 p45 (NF-E2)-related factor (Nrf2)/antioxidant response element (ARE) pathway was studied in vitro. Dose-dependent effects of pre-incubated flavonoids on pro-carcinogen 4-[(acetoxymethyl)nitrosamino]-1-(3-pyridyl)-1-butanone (NNKAc)-induced ROS and DNA damage in human bronchial epithelial cells were studied in comparison to non-flavonoids. The most effective flavonoids were assessed for the activation of Nrf2/ARE pathway. Genistein, procyanidin B2 (PCB2), and quercetin significantly suppressed the NNKAc-induced ROS and DNA damage. Quercetin significantly upregulated the phosphorylated protein kinase B/Akt. PCB2 significantly upregulated the activation of Nrf2 and Akt through phosphorylation. Genistein and PCB2 significantly upregulated the phospho-Nrf2 nuclear translocation and catalase activity. In summary, genistein and PCB2 reduced the NNKAc-induced ROS and DNA damage through the activation of Nrf2. Further studies are required to understand the role of dietary flavonoids on the regulation of the Nrf2/ARE pathway in relation to carcinogenesis.

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Genistein reduces NNKAc-induced ROS and DNA damage through activation of Nrf2.
“genistein has also been shown to reduce NNKAc-induced ROS and DNA damage through the activation of Nrf2”
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Authors: Suraweera, T. L., Merlin, J. P. J., Dellaire, G., Xu, Z., & Rupasinghe, H. P. V.
Affiliations: Dalhousie University (CA)
Corresponding author: Rupasinghe, H. P. Vasantha
ORCID: Suraweera, Tharindu L.
Merlin, J. P. Jose
Dellaire, Graham
Xu, Zhaolin
Rupasinghe, H. P. Vasantha
Venue: International Journal of Molecular Sciences
Publisher: MDPI AG
Published: 2023-02-12
Volume: 24
Issue: 4
Pages: 3676
ISSN: 1422-0067 (ISSN-L)
DOI: 10.3390/ijms24043676
PMC: PMC9961944
OpenAlex: W4320493371
Cited by: 35
References: 75
Citations by year: 2026: 8 · 2025: 10 · 2024: 11 · 2023: 6
FWCI: 3.11
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: International Journal of Molecular Sciences (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Funders: Discovery Grant of the Natural Sciences and Engineering Research Council (NSERC) of Canada (RGPIN2016 05369)
Beatrice Hunter Cancer Research Institute (RGPIN2016 05369)
CIBC (RGPIN2016 05369)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
Keywords: genistein, procyanidin B2, quercetin, Nrf2/ARE pathway, reactive oxygen species, DNA damage, bronchial epithelial cells, flavonoids, Akt signaling, catalase, Nrf2 nuclear translocation, chemoprevention, dietary flavonoids
MeSH terms: Carcinogens, Catechin, DNA Damage, Epithelial Cells, Flavonoids, Humans, Quercetin, Signal Transduction, Reactive Oxygen Species, Oxidative Stress, Genistein, Proanthocyanidins, Biflavonoids, Proto-Oncogene Proteins c-akt, NF-E2-Related Factor 2, Antioxidant Response Elements

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Genistein and Procyanidin B2 Reduce Carcinogen-Induced Reactive Oxygen Species and DNA Damage through the Activation of Nrf2/ARE Cell Signaling in Bronchial Epithelial Cells In Vitro

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