Cited by 462DNA Repair MechanismsEffects of Radiation ExposureRadiation Therapy and Dosimetry

Use of the γ-H2AX assay to monitor DNA damage and repair in translational cancer research

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Ivashkevich, Alesia, Redon, Christophe E., Nakamura, Asako J., Martin, Roger F., Martin, Olga A.·2012·Cancer Letters

Abstract

Formation of γ-H2AX in response to DNA double stranded breaks (DSBs) provides the basis for a sensitive assay of DNA damage in human biopsies. The review focuses on the application of γ-H2AX-based methods to translational studies to monitor the clinical response to DNA targeted therapies such as some forms of chemotherapy, external beam radiotherapy, radionuclide therapy or combinations thereof. The escalating attention on radiation biodosimetry has also highlighted the potential of the assay including renewed efforts to assess the radiosensitivity of prospective radiotherapy patients. Finally the γ-H2AX response has been suggested as a basis for an in vivo imaging modality.

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Crossref · 10.1016/j.canlet.2011.12.025

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  1. [62]Turner HC Brenner DJ Chen Y Bertucci A Zhang J Wang H Lyulko OV Xu Y Shuryak I Schaefer J Simaan N Randers-Pehrson G Yao YL Amundson SA Garty G Adapting the gamma-H2AX assay for automated processing in human lymphocytes. 1. Technological aspects Radiation research 175 2011 282 290 21388271 PMC3121903

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Authors
Ivashkevich, A., Redon, C. E., Nakamura, A. J., Martin, R. F., & Martin, O. A.
Affiliations
  • Peter MacCallum Cancer Centre (AU)
  • National Cancer Institute (US)
Corresponding author
Martin, Olga A.
Venue
Cancer Letters
Publisher
Elsevier BV
Published
2012-12
Volume
327
Issue
1-2
Pages
123-133
ISSN
0304-3835 (ISSN-L)
OpenAlex
W2099254999
Cited by
462
References
142
Citations by year
2026: 27 · 2025: 42 · 2024: 41 · 2023: 38 · 2022: 44 · 2021: 32 · 2020: 40 · 2019: 27 · 2018: 30 · 2017: 36 · 2016: 29 · 2015: 33 · 2014: 19 · 2013: 19 · 2012: 5
FWCI
1.25
Type
journal article
Language
en
Open access
Locations
  • Cancer Letters (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • National Cancer Institute (Z01BC006140)
  • National Institute of Allergy and Infectious Diseases
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
Keywords
γH2AX, DNA double-strand breaks, DNA damage, DNA repair, translational oncology, chemotherapy response, radiotherapy response, radionuclide therapy, biological dosimetry, radiosensitivity, in vivo imaging
MeSH terms
Animals, DNA Damage, DNA Repair, Histones, Humans, Neoplasms, Radiation Dosage, Radiation Tolerance, Biomarkers, Tumor, Molecular Imaging, High-Throughput Screening Assays, Translational Research, Biomedical

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