Use of the γ-H2AX assay to monitor DNA damage and repair in translational cancer research
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Formation of γ-H2AX in response to DNA double stranded breaks (DSBs) provides the basis for a sensitive assay of DNA damage in human biopsies. The review focuses on the application of γ-H2AX-based methods to translational studies to monitor the clinical response to DNA targeted therapies such as some forms of chemotherapy, external beam radiotherapy, radionuclide therapy or combinations thereof. The escalating attention on radiation biodosimetry has also highlighted the potential of the assay including renewed efforts to assess the radiosensitivity of prospective radiotherapy patients. Finally the γ-H2AX response has been suggested as a basis for an in vivo imaging modality.
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Phosphorylation of the histone H2A variant H2AX marks DNA double-strand breaks.
“The phosphorylation of this histone H2A variant indicates DSBs.27”
From Mdm2 inhibitors synergize with topoisomerase II inhibitors to induce p53-independent pancreatic cancer cell death (2013) · cites this paper as [27]
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Oxidatively generated complex DNA damage: Tandem and clustered lesions
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- Authors
- Ivashkevich, A., Redon, C. E., Nakamura, A. J., Martin, R. F., & Martin, O. A.
- Affiliations
- Peter MacCallum Cancer Centre (AU)
- National Cancer Institute (US)
- Corresponding author
- Martin, Olga A.
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 327
- Issue
- 1-2
- Pages
- 123-133
- ISSN
- 0304-3835 (ISSN-L)
- PMC
- PMC3329565
- OpenAlex
- W2099254999
- Cited by
- 462
- References
- 142
- Citations by year
- 2026: 27 · 2025: 42 · 2024: 41 · 2023: 38 · 2022: 44 · 2021: 32 · 2020: 40 · 2019: 27 · 2018: 30 · 2017: 36 · 2016: 29 · 2015: 33 · 2014: 19 · 2013: 19 · 2012: 5
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cancer Letters (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (Z01BC006140)
- National Institute of Allergy and Infectious Diseases
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Radiation Therapy and Dosimetry
- Keywords
- γH2AX, DNA double-strand breaks, DNA damage, DNA repair, translational oncology, chemotherapy response, radiotherapy response, radionuclide therapy, biological dosimetry, radiosensitivity, in vivo imaging
- MeSH terms
- Animals, DNA Damage, DNA Repair, Histones, Humans, Neoplasms, Radiation Dosage, Radiation Tolerance, Biomarkers, Tumor, Molecular Imaging, High-Throughput Screening Assays, Translational Research, Biomedical
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