The Neuroprotective Effects of Dendropanax morbifera Water Extract on Scopolamine-Induced Memory Impairment in Mice
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This study investigated the neuroprotective effects of Dendropanax morbifera leaves and stems (DMLS) water extract on scopolamine (SCO)-induced memory impairment in mice. First, we conducted experiments to determine the protective effect of DMLS on neuronal cells. Treatment with DMLS showed a significant protective effect against neurotoxicity induced by Aβ(25–35) or H2O2. After confirming the neuroprotective effects of DMLS, we conducted animal studies. We administered DMLS orally at concentrations of 125, 250, and 375 mg/kg for 3 weeks. In the Y-maze test, SCO decreased spontaneous alternation, but treatment with DMLS or donepezil increased spontaneous alternation. In the Morris water-maze test, the SCO-treated group showed increased platform reach time and decreased swim time on the target platform. The passive avoidance task found that DMLS ingestion increased the recognition index in short-term memory. Furthermore, memory impairment induced by SCO reduced the ability to recognize novel objects. In the Novel Object Recognition test, recognition improved with DMLS or donepezil treatment. In the mouse brain, except for the cerebellum, acetylcholinesterase activity increased in the SCO group and decreased in the DMLS and donepezil groups. We measured catalase and malondialdehyde, which are indicators of antioxidant effectiveness, and found that oxidative stress increased with SCO but was mitigated by DMLS or donepezil treatment. Thus, our findings suggest that ingestion of DMLS restored memory impairment by protecting neuronal cells from Aβ(25–35) or H2O2-induced neurotoxicity, and by reducing oxidative stress.
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Extracts from Nepeta nuda, Indigofera sessiliflora, Dendropanax morbifera, Pinus densiflora, Dichrocephala integrifolia, Alnus rugosa, Salvia moorcroftiana, and Sanguisorba minor reduced AChE activity and enhanced cognitive function in scopolamine-induced memory impairment models.
“Other studies on plant extracts like Nepeta nuda, Indigofera sessiliflora, Dendropanax morbifera, Pinus densiflora, Dichrocephala integrifolia, Alnus rugosa, Salvia moorcroftiana, and Sanguisorba minor have similarly reported reduced AChE activity and enhanced cognitive function in SCO-induced memory impairment models [40,41,42,43,44,45,46,47].”
From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [42]
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ALNasser, M. N., Alboraiy, G. M., Alsowig, E. M., & Alqattan, F. M. (2025) · Brain Sciences · doi:10.3390/brainsci15020215 · Cited by 35 · see citing works
- Authors
- Kim, S. B., Ryu, H. Y., Nam, W., Lee, S. M., Jang, M. R., Kwak, Y. G., Kang, G. I., Song, K. S., & Lee, J. W.
- Affiliations
- Korea Conformity Laboratories, Incheon 21999, Republic of Korea
- Huons (South Korea) (KR)
- Corresponding author
- Lee, Jae Won
- Venue
- International Journal of Molecular Sciences
- Publisher
- MDPI AG
- Published
- 2023-11-17
- Volume
- 24
- Issue
- 22
- Pages
- 16444
- ISSN
- 1422-0067 (ISSN-L)
- PMC
- PMC10671129
- OpenAlex
- W4388761854
- Cited by
- 8
- References
- 46
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 2
- FWCI
- 2.43
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- International Journal of Molecular Sciences (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministry of SMEs and Startups (S2945890)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Keywords
- Dendropanax morbifera, neuroprotection, scopolamine-induced amnesia, memory impairment, oxidative stress, acetylcholinesterase, donepezil, novel object recognition, passive avoidance, antioxidant activity, catalase, malondialdehyde, herbal water extract
- MeSH terms
- Donepezil, Acetylcholinesterase, Animals, Hydrogen Peroxide, Memory Disorders, Plant Extracts, Scopolamine, Water, Oxidative Stress, Neuroprotective Agents, Maze Learning, Mice
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