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Cited by 47Bone Tissue Engineering MaterialsTissue Engineering and Regenerative MedicineMedicinal Plant Pharmacodynamics Research

Promotion of Bone Regeneration Using Bioinspired PLGA/MH/ECM Scaffold Combined with Bioactive PDRN

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Kim, Da-Seul, Lee, Jun-Kyu, Jung, Ji-Won, Baek, Seung-Woon, Kim, Jun Hyuk, Heo, Yun, Kim, Tae-Hyung, Han, Dong Keun·2021·Materials
DOI 10.3390/ma14154149PMC PMC8348682
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Abstract

Current approaches of biomaterials for the repair of critical-sized bone defects still require immense effort to overcome numerous obstacles. The biodegradable polymer-based scaffolds have been required to expand further function for bone tissue engineering. Poly(lactic-co-glycolic) acid (PLGA) is one of the most common biopolymers owing to its biodegradability for tissue regenerations. However, there are major clinical challenges that the byproducts of the PLGA cause an acidic environment of implanting site. The critical processes in bone repair are osteogenesis, angiogenesis, and inhibition of excessive osteoclastogenesis. In this study, the porous PLGA (P) scaffold was combined with magnesium hydroxide (MH, M) and bone-extracellular matrix (bECM, E) to improve anti-inflammatory ability and osteoconductivity. Additionally, the bioactive polydeoxyribonucleotide (PDRN, P) was additionally incorporated in the existing PME scaffold. The prepared PMEP scaffold has pro-osteogenic and pro-angiogenic effects and inhibition of osteoclast due to the PDRN, which interacts with the adenosine A2A receptor agonist that up-regulates expression of vascular endothelial growth factor (VEGF) and down-regulates inflammatory cytokines. The PMEP scaffold has superior biological properties for human bone-marrow mesenchymal stem cells (hBMSCs) adhesion, proliferation, and osteogenic differentiation in vitro. Moreover, the gene expressions related to osteogenesis and angiogenesis of hBMSCs increased and the inflammatory factors decreased on the PMEP scaffold. In conclusion, it provides a promising strategy and clinical potential candidate for bone tissue regeneration and repairing bone defects.

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  • PDRN's effects are primarily mediated through upregulation of VEGF and IL-10, and downregulation of TNF-α, IL-6, HMGB1, and IL-1β.

    “These effects are primarily mediated through upregulation of vascular endothelial growth factor (VEGF) and interleukin (IL)-10, and downregulation of proinflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), IL-6, high mobility group box-1 (HMGB1), and IL-1β [71,72].”

    From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [72]

    Section 3.4 (page not provided) reports increased VEGF expression and reduced IL-6 and IL-1β expression with the PDRN-containing scaffold. The paper does not report the claimed changes in IL-10, TNF-α, or HMGB1, and sections 1 and 3.3 describe A2A-receptor stimulation and the salvage pathway rather than establishing the listed factors as PDRN’s primary mediators. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Kim, D.-S., Lee, J.-K., Jung, J.-W., Baek, S.-W., Kim, J. H., Heo, Y., Kim, T.-H., & Han, D. K.
Affiliations
  • CHA University (KR)
  • School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Korea
Corresponding author
Han, Dong Keun
ORCID
Venue
Materials
Publisher
MDPI AG
Published
2021-07-26
Volume
14
Issue
15
Pages
4149
ISSN
1996-1944 (ISSN-L)
DOI
10.3390/ma14154149
PMC
PMC8348682
OpenAlex
W3187042895
Cited by
47
References
0
Citations by year
2026: 4 · 2025: 11 · 2024: 11 · 2023: 14 · 2022: 7
FWCI
2.43
Type
journal article
Language
en
Open access
Licence
Locations
  • Materials (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Research Foundation of Korea funded by the Ministry of Science and ICT (MSIT) (2020R1A2B5B03002344, 2018M3A9E2024579)
  • Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health &amp; Welfare, Republic of Korea, the Ministry of Food and Drug Safety) (202011A05-05)
  • Ministry of Health &amp; Welfare Republic of Korea (HR16C0002)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Medicinal Plant Pharmacodynamics Research
Keywords
bone regeneration, PLGA scaffolds, magnesium hydroxide, bone matrix, polydeoxyribonucleotide, critical-size bone defect, osteogenic differentiation, angiogenesis, osteoclastogenesis, anti-inflammatory activity, adenosine A2A receptor, vascular endothelial growth factor, human bone marrow mesenchymal stem cells, biomimetic scaffolds, porous scaffolds

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