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Cited by 39Osteoarthritis Treatment and MechanismsProteoglycans and glycosaminoglycans researchPeriodontal Regeneration and Treatments

Effect of Polydeoxyribonucleotide on Angiogenesis and Wound Healing in an In Vitro Model of Osteoarthritis

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Baek, Ahreum, Kim, Yoon, Lee, Jin Woo, Lee, Sang Chul, Cho, Sung-Rae·2018·Cell Transplantation
DOI 10.1177/0963689718804130PMC PMC6299200
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Abstract

Osteoarthritis (OA) is degenerative disease, leading to pain and functional disability. It is reported that polydeoxyribonucleotide (PDRN) is a suitable therapy for OA. However, the therapeutic mechanisms of PDRN in OA are not fully understood. To investigate the effect of PDRN in an in vitro model of OA, interleukin (IL)-1β or phosphate-buffered saline (PBS) was used to treat a human chondrocytic cell line in hypoxic conditions for 24 h (IL-1β group or control group). PDRN was then used to treat IL-1β group cells for 24 h (PDRN group). By Label-Based Human Antibody Array 1000, angiopoietin-2 (ANG-2), platelet-derived growth factor (PDGF), angiostatin, and endostatin, which were related to angiogenesis, were chosen for further validation studies. Quantitative real-time reverse transcription polymerase chain reaction and western blot analysis validated that the levels of PDGF and ANG-2, which were related to pro-angiogenesis, were significantly increased in the PDRN group compared with those in the control group or the IL-1β group. However, the levels of endostatin and angiostatin, which were related in anti-angiogenesis, were significantly decreased in the PDRN group compared with those in the control group or the IL-1β group. In the same manner, vascular endothelial growth factor, which was a mediator of angiogenesis, was significantly increased in the PDRN group compared with those in the control group or the IL-1β group. Furthermore, wound closure was significantly increased in the PDRN group compared with the control group or the IL-1β group by in vitro scratch assay. Moreover, PDRN decreased expression of metalloproteinase 13, as a catabolic factor for OA, but increased expression of aggrecan, which was an anabolic factor for OA. These data suggest that PDRN may promote angiogenesis and wound healing via down-regulation of catabolism and up-regulation of anabolism in an in vitro model of OA.

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  • A2A receptor activation by PDRN reduces inflammatory cell infiltration, promotes proliferation and migration of reparative cells, stimulates VEGF production, and facilitates fibroblast differentiation and maturation.

    “A2A receptor activation by PDRN reduces inflammatory cell infiltration, promotes proliferation and migration of reparative cells, stimulates vascular endothelial growth factor (VEGF) production, and facilitates fibroblast differentiation and maturation, thereby accelerating the reparative cascade [112,113].”

    From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [113]

    The Introduction describes PDRN as activating the A2A receptor and enhancing cell proliferation in experimental healing models. The Results report increased VEGF expression and migration of chondrocytic cells after PDRN treatment in an in vitro osteoarthritis model. The paper does not establish reduced inflammatory-cell infiltration or fibroblast differentiation and maturation, and its migration findings do not cover reparative cells generally. (LLM, conf=0.89)

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Cited by

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Authors
Baek, A., Kim, Y., Lee, J. W., Lee, S. C., & Cho, S.-R.
Affiliations
Yonsei University (KR)
Corresponding author
  • Lee, Sang Chul
  • Cho, Sung-Rae
ORCID
Venue
Cell Transplantation
Publisher
SAGE Publications
Published
2018-10-12
Volume
27
Issue
11
Pages
1623-1633
ISSN
  • 0963-6897 (ISSN-L)
  • 1555-3892
DOI
10.1177/0963689718804130
PMC
PMC6299200
OpenAlex
W2897330919
Cited by
39
References
3
Citations by year
2026: 4 · 2025: 10 · 2024: 2 · 2023: 8 · 2022: 2 · 2021: 8 · 2020: 2 · 2019: 2
FWCI
2.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Transplantation (publishedVersion, cc-by-nc)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
Funders
Korea Health Industry Development Institute (HI16C1012)
Topics
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
Keywords
polydeoxyribonucleotide, osteoarthritis, angiogenesis, wound healing, IL-1β, chondrocytes, hypoxia, platelet-derived growth factor, angiopoietin-2, endostatin, angiostatin, vascular endothelial growth factor, matrix metalloproteinase-13, aggrecan, cartilage catabolism, cartilage anabolism

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