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Cited by 2Intestinal and Peritoneal AdhesionsSurgical Sutures and AdhesivesHernia repair and management

Polyvinylpyrrolidone–Alginate Film Barriers for Abdominal Surgery: Anti-Adhesion Effect in Murine Model

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Forysenkova, Anna A., Konovalova, Mariya V., Fadeeva, Inna V., Antonova, Olga S., Kotsareva, Olga D., Slonskaya, Tatiana K., Rau, Julietta V., Svirshchevskaya, Elena V.·2023·Materials
DOI 10.3390/ma16165532PMC PMC10456265
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Abstract

Surgical operations on the peritoneum are often associated with the formation of adhesions, which can interfere with the normal functioning of the internal organs. The effectiveness of existing barrier materials is relatively low. In this work, the effectiveness of soluble alginate–polyvinylpyrrolidone (PVP-Alg) and non-soluble Ca ion cross-linked (PVP-Alg-Ca) films in preventing these adhesions was evaluated. Experiments in vivo were performed on mice via mechanical injury to the adjacent peritoneum wall and the caecum, followed by the application of PVP-Alg or PVP-Alg-Ca films to the injured area. After 7 days, samples from the peritoneal wall and caecum were analyzed using histology and quantitative polymerase chain reaction (qPCR). It was shown that the expression of genes responsible for adhesion formation in the caecum in the PVP-Alg group was comparable to that in the control group, while in the PVP-Alg-Ca group, it increased by 5–10 times. These results were consistent with the histology: in the PVP-Alg group, the adhesions did not form, while in the PVP-Alg-Ca group, the adhesions corresponded to five points on the adhesion scale. Therefore, the formation of intraperitoneal adhesions can be effectively prevented by non-crosslinked, biodegradable PVP-Alg films, whereas cross-linked, not biodegradable PVP-Alg-Ca films cause inflammation and adhesion formation.

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  • In a mouse study, a soluble PVP-alginate film completely prevented adhesions: no macroscopic adhesions formed and gene expression of pro-adhesion markers remained at baseline levels, similar to uninjured controls.

    “After creating standardized peritoneal injuries in mice, the soluble PVP–Alg film completely prevented adhesions: no macroscopic adhesions formed and the gene expression of pro-adhesion markers remained at baseline levels, similar to uninjured controls.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [191]

    Results §3.2 reports that PVP-Alg dry films completely prevented visible adhesions in the mouse model. But Results §3.3 reports residual adhesions on histology, and the Conclusions report increased expression of several genes in the PVP-Alg group compared with control animals. The claim that pro-adhesion gene expression remained at uninjured baseline levels is therefore not supported. (LLM, conf=0.90)

    Experimental — accuracy not yet measured

  • In the same mouse study, a Ca-crosslinked alginate film (not readily biodegradable) induced a robust foreign-body response-adhesion bands scored as severe (grade 5) and a 5-10× upregulation of adhesion-related genes occurred.

    “In contrast, the Ca-crosslinked alginate film (not readily biodegradable) induced a robust foreign-body response—adhesion bands scored as severe (grade 5) and a 5–10× upregulation of adhesion-related genes occurred.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [191]

  • The PVP-alginate study was short term (7 days) in a murine model.

    “One limitation of this study is that this experiment was short term (7 days) in a murine model.”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [191]

  • A non-crosslinked, fast-dissolving alginate barrier is effective, whereas a persistent alginate scaffold can trigger inflammation and adhesion formation.

    “These results underscore that a non-crosslinked, fast-dissolving alginate barrier is effective, whereas a persistent alginate scaffold can trigger inflammation and adhesion formation (Figure 9B) [191].”

    From Biomaterials in Postoperative Adhesion Barriers and Uterine Tissue Engineering (2025) · cites this paper as [191]

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Sorted by citations

Authors
Forysenkova, A. A., Konovalova, M. V., Fadeeva, I. V., Antonova, O. S., Kotsareva, O. D., Slonskaya, T. K., Rau, J. V., & Svirshchevskaya, E. V.
Affiliations
  • Baikov Institute of Metallurgy and Materials Science (RU)
  • Institute of Bioorganic Chemistry (RU)
  • Sechenov University (RU)
Corresponding author
  • Rau, Julietta V.
  • Svirshchevskaya, Elena V.
ORCID
Venue
Materials
Publisher
MDPI AG
Published
2023-08-09
Volume
16
Issue
16
Pages
5532
ISSN
1996-1944 (ISSN-L)
DOI
10.3390/ma16165532
PMC
PMC10456265
OpenAlex
W4385709059
Cited by
2
References
0
Citations by year
2025: 1 · 2024: 1
FWCI
0.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Materials (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Russian Scientific Fund (22-23-00278)
Topics
  • Health Sciences › Medicine › Surgery › Intestinal and Peritoneal Adhesions
  • Health Sciences › Medicine › Surgery › Surgical Sutures and Adhesives
  • Health Sciences › Medicine › Surgery › Hernia repair and management
Keywords
polyvinylpyrrolidone, alginate, surgical adhesions, calcium crosslinking, peritoneal injury, cecum, biodegradable films, inflammation, abdominal surgery

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