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Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum
Open accessAbstract
Microalgae have been emerging as an important source for the production of bioactive compounds. Marine diatoms can store high amounts of lipid and grow quite quickly. However, the genetic and biochemical characteristics of fatty acid biosynthesis in diatoms remain unclear. Glycerophospholipids are integral as structural and functional components of cellular membranes, as well as precursors of various lipid mediators. In addition, diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the last step of triacylglyceride (TAG) biosynthesis. However, a comprehensive sequence-structure and functional analysis of DGAT in diatoms is lacking. In this study, an isoform of diacylglycerol acyltransferase type 2 of the marine diatom Phaeodactylum tricornutum was characterized. Surprisingly, DGAT2 overexpression in P. tricornutum stimulated more oil bodies, and the neutral lipid content increased by 35%. The fatty acid composition showed a significant increase in the proportion of polyunsaturated fatty acids; in particular, EPA was increased by 76.2%. Moreover, the growth rate of transgenic microalgae remained similar, thereby maintaining a high biomass. Our results suggest that increased DGAT2 expression could alter fatty acid profile in the diatom, and the results thus represent a valuable strategy for polyunsaturated fatty acid production by genetic manipulation.
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Genetic Engineering of Algae for Enhanced Biofuel Production
Radakovits, R., Jinkerson, R. E., Darzins, A., & Posewitz, M. C. (2010) · Eukaryotic Cell · doi:10.1128/ec.00364-09 · Cited by 1,142
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Sustainability of the four generations of biofuels – A review
Mat Aron, N. S., Khoo, K. S., Chew, K. W., Show, P. L., Chen, W., & Nguyen, T. H. P. (2020) · International Journal of Energy Research · doi:10.1002/er.5557 · Cited by 479
The lipid biochemistry of eukaryotic algae
Li-Beisson, Y., Thelen, J. J., Fedosejevs, E., & Harwood, J. L. (2019) · Progress in Lipid Research · doi:10.1016/j.plipres.2019.01.003 · Cited by 453
Minhas, A. K., Hodgson, P., Barrow, C. J., & Adholeya, A. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00546 · Cited by 445
Khoo, K. S., Ahmad, I., Chew, K. W., Iwamoto, K., Bhatnagar, A., & Show, P. L. (2023) · Progress in Energy and Combustion Science · doi:10.1016/j.pecs.2023.101071 · Cited by 363
Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2014.10.002 · Cited by 321
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Metadata
- Authors
- Niu, Y.-F., Zhang, M.-H., Li, D.-W., Yang, W.-D., Liu, J.-S., Bai, W.-B., & Li, H.-Y.
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2013-11-13
- Volume
- 11
- Issue
- 11
- Pages
- 4558-4569
- ISSN
- 1660-3397 (ISSN-L)
- OpenAlex
- W2052225110
- Cited by
- 279
- References
- 1
- FWCI
- 5.76
- Type
- journal article
- Language
- en
- Open access
- gold
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- Niu, Y.-F., Zhang, M.-H., Li, D.-W., Yang, W.-D., Liu, J.-S., Bai, W.-B., & Li, H.-Y. (2013). Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum. Marine Drugs. https://doi.org/10.3390/md11114558
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