Cited by 321Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionLipid metabolism and biosynthesis

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

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Xue, Jiao, Niu, Ying-Fang, Huang, Tan, Yang, Wei-Dong, Liu, Jie-Sheng, Li, Hong-Ye·2015·Metabolic Engineering

Abstract

To obtain fast growing oil-rich microalgal strains has been urgently demanded for microalgal biofuel. Malic enzyme (ME), which is involved in pyruvate metabolism and carbon fixation, was first characterized in microalgae here. Overexpression of Phaeodactylum tricornutum ME (PtME) significantly enhanced the expression of PtME and its enzymatic activity in transgenic P. tricornutum. The total lipid content in transgenic cells markedly increased by 2.5-fold and reached a record 57.8% of dry cell weight with a similar growth rate to wild type, thus keeping a high biomass. The neutral lipid content was further increased by 31% under nitrogen-deprivation treatment, still 66% higher than that of wild type. Transgenic microalgae cells exhibited obvious morphological changes, as the cells were shorter and thicker and contained larger oil bodies. Immuno-electron microscopy targeted PtME to the mitochondrion. This study markedly increased the oil content in microalgae, suggesting a new route for developing ideal microalgal strains for industrial biodiesel production.

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Authors
Xue, J., Niu, Y.-F., Huang, T., Yang, W.-D., Liu, J.-S., & Li, H.-Y.
Affiliations
Jinan University (CN)
Venue
Metabolic Engineering
Publisher
Elsevier BV
Published
2015-01
Volume
27
Pages
1-9
ISSN
1096-7176 (ISSN-L)
OpenAlex
W1986227133
Cited by
321
References
51
Citations by year
2026: 12 · 2025: 20 · 2024: 22 · 2023: 31 · 2022: 30 · 2021: 41 · 2020: 34 · 2019: 36 · 2018: 26 · 2017: 27 · 2016: 29 · 2015: 13
FWCI
7.59
Type
journal article
Language
en
Open access
Locations
  • Metabolic Engineering (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
  • National Science and Technology Support Program (2011BAD14B03)
  • Science and Technology Project of Guangdong (2009B050600005, 2010B030600005)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
Phaeodactylum tricornutum, malic enzyme, neutral lipid accumulation, transgenic microalgae, nitrogen starvation, oil bodies, mitochondrial targeting, pyruvate metabolism, CO2 fixation, microalgae biodiesel, genetic improvement
MeSH terms
Genetic Engineering, Malate Dehydrogenase, Gene Expression, Diatoms, Algal Proteins, Organisms, Genetically Modified, Lipid Metabolism

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