Sulfated Hetero-Polysaccharides Protect SH-SY5Y Cells from H2O2-Induced Apoptosis by Affecting the PI3K/Akt Signaling Pathway
Open accessAbstract
Parkinson’s disease (PD) is one of the most common neurodegenerative diseases. Recent studies suggest that sulfated hetero-polysaccharides (UF) protect against developing PD. However, the detailed mechanisms of how UF suppress neuronal death have not been fully elucidated. We investigated the cytoprotective mechanisms of UF using human dopaminergic neuroblastoma SH-SY5Y cells as a PD model. UF prevented H2O2-induced apoptotic cell death in SH-SY5Y cells in a dose-dependent manner. An examination of the PI3K/Akt upstream pathway revealed that UF-pretreated cells showed a decreased relative density of Akt, PI3K, and TrkA, and increased the phosphorylation of Akt, PI3K, and NGF; the PI3K inhibitor, LY294002, partially prevented this effect. An examination of the PI3K/Akt downstream pathway revealed the increased expression of the apoptosis-associated markers Bax, p53, CytC, and GSK3β, and the decreased expression of Bcl-2 in UF-treated cells. UF-treated cells also exhibited decreased caspase-3, caspase-8, and caspase-9 activities, which induced cell apoptosis. Our results demonstrate that UF affect the PI3K/Akt pathway, as well as downstream signaling. Therefore, the UF-mediated activation of PI3K/Akt could provide a new potential therapeutic strategy for neurodegenerative diseases associated with oxidative injury. These findings contribute to a better understanding of the critical roles of UF in the treatment of PD.
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In vitro models with neurons isolated from the CNS and/or immortalized cell lines demonstrated that H2O2 causes cell death through apoptosis.
“Various in vitro models carried out with neurons isolated from the CNS and/or with immortalized cell lines, demonstrated that H2O2 causes cell death through apoptosis [118,131,132,133,134,135].”
From Multi-Target Mechanisms of Phytochemicals in Alzheimer’s Disease: Effects on Oxidative Stress, Neuroinflammation and Protein Aggregation (2022) · cites this paper as [133]
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- Authors
- Wang, J., Liu, H., Zhang, X., Li, X., Geng, L., Zhang, H., & Zhang, Q.
- Affiliations
- Chinese Academy of Sciences (CN)
- Nantong University (CN)
- Taian City Central Hospital (CN)
- Corresponding author
- Zhang, Quanbin
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2017-04-06
- Volume
- 15
- Issue
- 4
- Pages
- 110
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC5408256
- OpenAlex
- W2605172937
- Cited by
- 41
- References
- 34
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 9 · 2021: 2 · 2020: 4 · 2019: 7 · 2018: 4 · 2017: 1
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (41406144)
- Chinese Academy of Sciences
- Youth Innovation Promotion Association (2016190)
- Topics
- Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- Parkinson's disease, SH-SY5Y cells, hydrogen peroxide-induced apoptosis, PI3K/Akt signaling, neuroprotection, TrkA, nerve growth factor, caspase activation, Bax, Bcl-2, GSK3β, oxidative stress, neurodegeneration
- MeSH terms
- Cell Line, Humans, Hydrogen Peroxide, Neuroblastoma, Neurons, Parkinson Disease, Polysaccharides, Sulfates, Signal Transduction, Apoptosis, Neuroprotective Agents, Phosphatidylinositol 3-Kinases, Caspases, Proto-Oncogene Proteins c-akt
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