Specific molecular signatures for type ii crustins in penaeid shrimp uncovered by the identification of crustin-like antimicrobial peptides in Litopenaeus vannamei
Open accessAbstract
Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or “Crustins” and Type IIb or “Crustin-like”) possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in penaeid shrimp. To gain insight into the molecular diversity of Type II crustins in penaeids, we identified and characterized a Type IIb crustin in Litopenaeus vannamei (Crustin-like Lv) and compared Type II crustins at both molecular and transcriptional levels. Although L. vannamei Type II crustins (Crustin Lv and Crustin-like Lv) are encoded by separate genes, they showed a similar tissue distribution (hemocytes and gills) and transcriptional response to the shrimp pathogens Vibrio harveyi and White spot syndrome virus (WSSV). As Crustin Lv, Crustin-like Lv transcripts were found to be present early in development, suggesting a maternal contribution to shrimp progeny. Altogether, our in silico and transcriptional data allowed to conclude that (1) each sub-type displays a specific amino acid signature at the C-terminal end holding both the cysteine-rich region and the whey acidic protein (WAP) domain, and that (2) shrimp Type II crustins evolved from a common ancestral gene that conserved a similar pattern of transcriptional regulation.
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Type I crustins are widely distributed across decapod crustaceans (Pleocyemata and Dendrobranchiata), while Type II crustins (Sub-Types IIa and IIb) are mainly present in penaeid shrimps (Dendrobranchiata).
“Interestingly, while Type I crustins are widely distributed across decapod crustaceans (Pleocyemata and Dendrobranchiata), Type II crustins (Sub-Types IIa and IIb) are mainly present in penaeid shrimps (Dendrobranchiata) (46).”
From Antimicrobial Peptides and Ectosymbiotic Relationships: Involvement of a Novel Type IIa Crustin in the Life Cycle of a Deep-Sea Vent Shrimp (2020) · cites this paper as [46]
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Antimicrobial peptides in crustacean, especially in Procambarus clarkii (crayfish)
Yang, Q., Li, H., Hou, L., Zhu, L., & Kong, X. (2025) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2025.110646 · Cited by 8 · see citing works
Dai, X., Nie, X., Han, Z., Ren, Q., & Huang, X. H. (2023) · Aquaculture · doi:10.1016/j.aquaculture.2023.739275 · Cited by 7 · see citing works
Bao, M., Liang, Y., Lang, L., Chen, C. M., Zhang, Z., & Wang, L. (2023) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2023.108559 · Cited by 6 · see citing works
Andriawan, S., Bao, H. T., Purbiantoro, W., Yuniarti, A., Chaung, H. C., Yen, T., & Cheng, T. (2023) · Aquaculture International · doi:10.1007/s10499-023-01058-y · Cited by 5 · see citing works
“The midgut is the main absorptive area of the digestive tract, which represents a vital route of pathogen entry (Barreto et al. 2018; Rosa and Barracco 2010) and is closely associated with hemocytes.”
BackgroundZhou, Y., Song, Q., Liu, Y., Sun, Y., & Zhang, J. (2022) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2022.06.003 · Cited by 4 · see citing works
Aboul-Ella, H., Aboul-Ella, H., & Zoysa, M. D. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-026-10959-2 · Cited by 3 · see citing works
Jiang, H., Zhang, S., Liu, X., Li, Y., Li, H., Zhang, R., Li, Y., Zhang, M., Wang, L., Yu, M., et al. (2023) · Aquaculture · doi:10.1016/j.aquaculture.2023.739825 · Cited by 3 · see citing works
Shrimp immune responses and antimicrobial peptides: an overview with emphasis on Vibrio infection
Radhakrishnan, N. K., Panigrahi, A., Rajeshwar, B. N., & Bedekar, M. K. (2026) · Aquaculture International · doi:10.1007/s10499-026-02444-y · Cited by 2 · see citing works
“A type 2 crustin peptide have been reported in several penaeid shrimp species, including Fi-crustin2 (ARS65735) from P. indicus (Sruthy et al. 2017); Crustin type-IIa, CruIIa-3 (AFV77524) (Barreto et al. 2018); and Crustin type-IIb subtypes, LvCruIIb-1 (QOL09966), LvCruIIb-2 (QOL09967), and LvCruIIb-3 (QOL09968), all…”
Argenta, N., Perazzolo, L. M., & Rosa, R. D. (2023) · Aquaculture International · doi:10.1007/s10499-023-01346-7 · Cited by 1 · see citing works
“…et al. 2020), but transcripts of some members have also been detected in other specialized immune-related tissues, such as gills (Jiang et al. 2015; Barreto et al. 2018), epipodites (Vatanavicharn et al. 2009), epidermis (Li et al. 2020; Lv et al. 2020a), stomach (Jiang et al. 2015; Li et al.…”
Noorjahan, K. K., Thomas, A. M., Lenin, K. L. D., Athira, M. T., Greeshma, T. J., & Antony, S. P. (2025) · Ecological Genetics and Genomics · doi:10.1016/j.egg.2025.100338 · Cited by 1 · see citing works
Ananya, B., Apurva, V. G., Karunasagar, I., Chakraborty, A., & Maiti, B. (2026) · Comparative Immunology Reports · doi:10.1016/j.cirep.2026.200271 · Cited by 1 · see citing works
“Type IIb crustins within a single species are diverse, reflecting distinct evolutionary pressures and possible functional specializations [134].”
Argenta, N., Perazzolo, L. M., & Rosa, R. D. (2023) · SSRN Electronic Journal · doi:10.2139/ssrn.4587882
Neelima, S., Mohan, A. S., Kesavan, D., Muneer, A., & Philip, R. (2025) · International Journal of Peptide Research and Therapeutics · doi:10.1007/s10989-025-10749-4
“Their classification has continued to evolve, with recent studies suggesting up to seven main types based on structural and phylogenetic criteria (Smith et al. 2008; Li et al. 2020 ; Matos and Rosa 2022 ; Barreto et al. 2018).”
Betancourt, J. L., Méndez, L. R., Rodríguez, Y., Rodríguez‐Ramos, T., Carpio, Y., Estrada, M. P., Ramos, L., & Dixon, B. (2025) · Aquaculture Reports · doi:10.1016/j.aqrep.2025.103187
Betancourt, J. L., Ihedimbu, I., Rodríguez‐Ramos, T., Coronado-Molina, D. E., Carpio, Y., Estrada, M. P., Hernández‐López, J., Ramos, L., & Dixon, B. (2025) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2025.111047
Methou, P. (2019) · theses.fr (ABES) · doi:10.70675/bf7aab7fzc361z40c2z9ac6z34c7f7b317db · Cited by 1 · see citing works
- Authors
- Barreto, C., Coelho, J., Yuan, J., Xiang, J., Perazzolo, L., & Rosa, R.
- Affiliations
- Universidade Federal de Santa Catarina (BR)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Rosa, Rafael
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2018-01-16
- Volume
- 16
- Issue
- 1
- Pages
- 31
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC5793079
- OpenAlex
- W2788273327
- Cited by
- 40
- References
- 45
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 1 · 2023: 7 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 4 · 2018: 2
- FWCI
- 1.72
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (PVE 401191/2014-1, Universal 406530/2016-5)
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CIMAR 1974/2014)
- National High-Technology Research and Development Program of China (863 program; 2012AA10A404, 2012AA092205)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- crustin, antimicrobial peptides, Litopenaeus vannamei, penaeid shrimp, cysteine-rich region, gene evolution, transcriptional regulation, Vibrio harveyi, white spot syndrome virus, hemocytes, gill, maternal gene expression, shrimp immunity
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Hemocytes, Milk Proteins, Phylogeny, Transcription, Genetic, Vibrio, Sequence Alignment, Antimicrobial Cationic Peptides, Penaeidae, White spot syndrome virus 1
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