Microalgae-based 3D bioprinting: recent advances, applications and perspectives
Open accessAbstract
Three-dimensional bioprinting integrating living cells and bioactive materials enables the fabrication of scaffold structures supporting diverse cellular growth and metabolism. Microalgae are among the most promising microbial platforms for the construction of photosynthetic cell factories, while the current industrial-scale cultivation of microalgae remains predominantly dependent on traditional liquid submerged systems, imposing limitations on commercial viability due to both process and economic constraints. Encapsulation of microalgae within bioactive matrices combined with 3D bioprinting to fabricate customized structures has been explored to address the limitations of submerged cultivation, which are expected to expand microalgal applications and establish new research directions in microalgal biotechnology. This review analyzes both matrices and methods of 3D bioprinting, summarizing the advancement of microalgae-based 3D bioprinting into six main domains including living building materials, biophotovoltaics, photosynthetic biosynthesis, bioremediation, tissue engineering, and food engineering. Lastly, synthetic biology-informed perspectives are provided on future developments of 3D bioprinting technologies and their potential in microalgal research.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Inkjet-based systems do not involve direct contact; bioink droplets are applied to predefined locations with piezoelectric or thermal actuators.
“Finally, inkjet-based systems do not involve direct contact (Figure 5C); bioink droplets are applied to predefined locations with the aid of piezoelectric or thermal actuators (Tang et al., 2025).”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Tang et al., 2025
There are three approaches to bioprinting: extrusion-based systems, light-projection-based systems, and inkjet-based systems.
“There are essentially three approaches to bioprinting: extrusion-based systems, light-projection-based systems, and inkjet-based systems, as illustrated in Figure 3.”
From Microalgae biotechnology and its role in sustainable and healthy food design (2025) · cites this paper as Tang et al., 2025
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Chisti, Y. (2007) · Biotechnology Advances · doi:10.1016/j.biotechadv.2007.02.001 · Cited by 9,524 · see citing works
25th Anniversary Article: Engineering Hydrogels for Biofabrication
Malda, J., Visser, J., Melchels, F. P., Jüngst, T., Hennink, W. E., Dhert, W. J. A., Groll, J., & Hutmacher, D. W. (2013) · Advanced Materials · doi:10.1002/adma.201302042 · Cited by 1,901 · see citing works
Hinton, T. J., Jallerat, Q., Palchesko, R. N., Park, J. H., Grodzicki, M. S., Shue, H.-J., Ramadan, M. H., Hudson, A. R., & Feinberg, A. W. (2015) · Science Advances · doi:10.1126/sciadv.1500758 · Cited by 1,805 · see citing works
Comparing Photosynthetic and Photovoltaic Efficiencies and Recognizing the Potential for Improvement
Blankenship, R. E., Tiede, D. M., Barber, J., Brudvig, G. W., Fleming, G., Ghirardi, M., Gunner, M. R., Junge, W., Kramer, D. M., Melis, A., et al. (2011) · Science · doi:10.1126/science.1200165 · Cited by 1,638 · see citing works
Markstedt, K., Mantas, A., Tournier, I., Martínez Ávila, H., Hägg, D., & Gatenholm, P. (2015) · Biomacromolecules · doi:10.1021/acs.biomac.5b00188 · Cited by 1,528 · see citing works
Three-dimensional bioprinting of thick vascularized tissues
Kolesky, D. B., Homan, K. A., Skylar-Scott, M. A., & Lewis, J. A. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1521342113 · Cited by 1,492 · see citing works
Flombaum, P., Gallegos, J. L., Gordillo, R. A., Rincón, J., Zabala, L. L., Jiao, N., Karl, D. M., Li, W. K. W., Lomas, M. W., Veneziano, D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1307701110 · Cited by 1,438 · see citing works
Williams, C. G., Malik, A. N., Kim, T. K., Manson, P. N., & Elisseeff, J. H. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.04.024 · Cited by 903 · see citing works
Xu, T., Binder, K. W., Albanna, M. Z., Dice, D. D., Zhao, W., Yoo, J. J., & Atala, A. (2012) · Biofabrication · doi:10.1088/1758-5082/5/1/015001 · Cited by 633 · see citing works
Volumetric Bioprinting of Complex Living‐Tissue Constructs within Seconds
Bernal, P. N., Delrot, P., Loterie, D., Li, Y., Malda, J., Moser, C., & Levato, R. (2019) · Advanced Materials · doi:10.1002/adma.201904209 · Cited by 618 · see citing works
The role of phytoplankton photosynthesis in global biogeochemical cycles
Falkowski, P. G. (1994) · Photosynthesis Research · doi:10.1007/bf00014586 · Cited by 571 · see citing works
Nguyen, P. Q., Courchesne, N. D., Duraj‐Thatte, A., Praveschotinunt, P., & Joshi, N. S. (2018) · Advanced Materials · doi:10.1002/adma.201704847 · Cited by 566 · see citing works
Novel Materials for 3D Printing by Photopolymerization
Layani, M., Wang, X., & Magdassi, S. (2018) · Advanced Materials · doi:10.1002/adma.201706344 · Cited by 565 · see citing works
Microalgae immobilization: Current techniques and uses
Moreno‐Garrido, I. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2007.05.040 · Cited by 487 · see citing works
An Introduction to 3D Bioprinting: Possibilities, Challenges and Future Aspects
Kačarević, Ž. P., Rider, P. M., Alkildani, S., Retnasingh, S., Smeets, R., Jung, O., Ivanišević, Z., & Barbeck, M. (2018) · Materials · doi:10.3390/ma11112199 · Cited by 486 · see citing works
Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Chen, H., Cheng, Y., Tian, J., Yang, P., Zhang, X., Chen, Y., Hu, Y., & Wu, J. (2020) · Science Advances · doi:10.1126/sciadv.aba4311 · Cited by 464 · see citing works
Comparison of microalgal biomass profiles as novel functional ingredient for food products
Batista, A. P., Gouveia, L., Bandarra, N. M., Franco, J. M., & Raymundo, A. (2013) · Algal Research · doi:10.1016/j.algal.2013.01.004 · Cited by 450 · see citing works
Bioprinting of hybrid tissue constructs with tailorable mechanical properties
Schuurman, W., Khristov, V., Pot, M. W., van Weeren, P. R., Dhert, W. J. A., & Malda, J. (2011) · Biofabrication · doi:10.1088/1758-5082/3/2/021001 · Cited by 411 · see citing works
Microoxen: Microorganisms to move microscale loads
Weibel, D. B., Garstecki, P., Ryan, D., DiLuzio, W. R., Mayer, M., Seto, J. E., & Whitesides, G. M. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0505481102 · Cited by 408 · see citing works
Zhang, Y. S., Haghiashtiani, G., Hübscher, T., Kelly, D. J., Lee, J. M., Lütolf, M. P., McAlpine, M. C., Yeong, W. Y., Zenobi‐Wong, M., & Malda, J. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-021-00073-8 · Cited by 407 · see citing works
Vat polymerization-based bioprinting—process, materials, applications and regulatory challenges
Ng, W. L., Lee, J. M., Zhou, M., Chen, Y., Lee, A. K.-X., Yeong, W. Y., & Shen, Y. (2019) · Biofabrication · doi:10.1088/1758-5090/ab6034 · Cited by 403 · see citing works
A 3D bioprinted complex structure for engineering the muscle–tendon unit
Merceron, T. K., Burt, M. A., Seol, Y., Kang, H.-W., Lee, S. J., Yoo, J. J., & Atala, A. (2015) · Biofabrication · doi:10.1088/1758-5090/7/3/035003 · Cited by 373 · see citing works
Light energy to bioelectricity: photosynthetic microbial fuel cells
Rosenbaum, M. A., He, Z., & Angenent, L. T. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.03.010 · Cited by 357 · see citing works
Freeform inkjet printing of cellular structures with bifurcations
Christensen, K., Xu, C., Chai, W., Zhang, Z., Fu, J., & Huang, Y. H. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25501 · Cited by 352 · see citing works
Daly, A. C., Prendergast, M. E., Hughes, A. J., & Burdick, J. A. (2021) · Cell · doi:10.1016/j.cell.2020.12.002 · Cited by 345 · see citing works
3D printing with cellulose materials
Wang, Q., Sun, J., Yao, Q., Ji, C., Liu, J., & Zhu, Q. (2018) · Cellulose · doi:10.1007/s10570-018-1888-y · Cited by 325 · see citing works
Self-citationProgrammable and printable Bacillus subtilis biofilms as engineered living materials
Huang, J., Liu, S., Zhang, C., Wang, X., Pu, J., Ba, F., Xue, S., Ye, H., Zhao, T., Li, K., et al. (2018) · Nature Chemical Biology · doi:10.1038/s41589-018-0169-2 · Cited by 315 · see citing works
Biophotovoltaics: oxygenic photosynthetic organisms in the world of bioelectrochemical systems
McCormick, A. J., Bombelli, P., Bradley, R. W., Thorne, R. J., Wenzel, T., & Howe, C. J. (2015) · Energy & Environmental Science · doi:10.1039/c4ee03875d · Cited by 311 · see citing works
Dynamic phototuning of 3D hydrogel stiffness
Stowers, R. S., Allen, S. C., & Suggs, L. J. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1421897112 · Cited by 309 · see citing works
Photon management for augmented photosynthesis
Ooms, M. D., Dinh, C., Sargent, E. H., & Sinton, D. A. (2016) · Nature Communications · doi:10.1038/ncomms12699 · Cited by 298 · see citing works
Bio-resin for high resolution lithography-based biofabrication of complex cell-laden constructs
Lim, K. S., Levato, R., Costa, P. F., Castilho, M. D., Alcala‐Orozco, C. R., van Dorenmalen, K. M. A., Melchels, F. P. W., Gawlitta, D., Hooper, G. J., Malda, J., et al. (2018) · Biofabrication · doi:10.1088/1758-5090/aac00c · Cited by 297 · see citing works
Engineered algae: A novel oxygen-generating system for effective treatment of hypoxic cancer
Qiao, Y., Yang, F., Xie, T., Du, Z. H., Zhong, D., Qi, Y., Li, Y., Li, W., Lu, Z., Rao, J., et al. (2020) · Science Advances · doi:10.1126/sciadv.aba5996 · Cited by 286 · see citing works
Concrete needs to lose its colossal carbon footprint
(2021) · Nature · doi:10.1038/d41586-021-02612-5 · Cited by 267 · see citing works
3D food printing of as the new way of preparing food: A review
Mantihal, S., Rovina, K., & Lee, B. (2020) · International Journal of Gastronomy and Food Science · doi:10.1016/j.ijgfs.2020.100260 · Cited by 250 · see citing works
Biomineralization and Successive Regeneration of Engineered Living Building Materials
Heveran, C. M., Williams, S. L., Qiu, J., Artier, J., Hubler, M. H., Cook, S. M., Cameron, J. C., & Srubar, W. V. (2020) · Matter · doi:10.1016/j.matt.2019.11.016 · Cited by 232 · see citing works
The potentials and challenges of using microalgae as an ingredient to produce meat analogues
Fu, Y., Chen, T., Chen, S. H. Y., Liu, B., Sun, P., Sun, H., & Chen, F. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2021.03.050 · Cited by 229 · see citing works
Guillemot, F., Mironov, V. A., & Nakamura, M. (2010) · Biofabrication · doi:10.1088/1758-5082/2/1/010201 · Cited by 225 · see citing works
Micro-sized microbial fuel cell: A mini-review
Wang, H.-Y. A., Bernarda, A., Huang, C., Lee, D., & Chang, J. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.07.007 · Cited by 224 · see citing works
Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization
Rademakers, T., Horvath, J. M., van Blitterswijk, C., & LaPointe, V. L. S. (2019) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2932 · Cited by 219 · see citing works
Bioprinting 101: Design, Fabrication, and Evaluation of Cell-Laden 3D Bioprinted Scaffolds
Deo, K. A., Singh, K. A., Peak, C. W., Alge, D. L., & Gaharwar, A. K. (2020) · Tissue Engineering Part A · doi:10.1089/ten.tea.2019.0298 · Cited by 199 · see citing works
Three-dimensional printing of biological matters
Munaz, A., Vadivelu, R. K., John, J. A. S., Barton, M., Kamble, H., & Nguyen, N. (2016) · Journal of Science Advanced Materials and Devices · doi:10.1016/j.jsamd.2016.04.001 · Cited by 179 · see citing works
Electricity generation from digitally printed cyanobacteria
Sawa, M., Fantuzzi, A., Bombelli, P., Howe, C. J., Hellgardt, K., & Nixon, P. J. (2017) · Nature Communications · doi:10.1038/s41467-017-01084-4 · Cited by 173 · see citing works
Vieira, M. V., Oliveira, S. M., Amado, I. R., Fasolin, L. H., Vicente, A. A., Pastrana, L. M., & Fuciños, P. (2020) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2020.105893 · Cited by 159 · see citing works
Lode, A., Krujatz, F., Brüggemeier, S., Quade, M., Schütz, K., Knaack, S., Weber, J., Bley, T., & Gelinsky, M. (2015) · Engineering in Life Sciences · doi:10.1002/elsc.201400205 · Cited by 157 · see citing works
Wangpraseurt, D., You, S., Azam, F., Jacucci, G., Gaidarenko, O., Hildebrand, M., Kühl, M., Smith, A. G., Davey, M. P., Smith, A., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-15486-4 · Cited by 149 · see citing works
In Situ 3D Bioprinting Living Photosynthetic Scaffolds for Autotrophic Wound Healing
Wang, X., Yang, C., Yu, Y., & Zhao, Y. (2022) · Research · doi:10.34133/2022/9794745 · Cited by 143 · see citing works
An innovative biologic system for photon-powered myocardium in the ischemic heart
Cohen, J. E., Goldstone, A. B., Paulsen, M. J., Shudo, Y., Steele, A. N., Edwards, B. B., Patel, J. B., MacArthur, J. W., Hopkins, M. S., Burnett, C. E., et al. (2017) · Science Advances · doi:10.1126/sciadv.1603078 · Cited by 141 · see citing works
Engineered Living Materials: Taxonomies and Emerging Trends
Srubar, W. V. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.10.009 · Cited by 138 · see citing works
Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions
Ma, Z., Cheah, W. Y., Ng, I., Chang, J., Zhao, M., & Show, P. L. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.09.002 · Cited by 138 · see citing works
3D-printed hierarchical pillar array electrodes for high-performance semi-artificial photosynthesis
Chen, X., Lawrence, J. M., Wey, L. T., Schertel, L., Jing, Q., Vignolini, S., Howe, C. J., Kar‐Narayan, S., & Zhang, J. (2022) · Nature Materials · doi:10.1038/s41563-022-01205-5 · Cited by 137 · see citing works
Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization
Song, Q., Jiao, K., Tonggu, L., Wang, L., Zhang, S., Yang, Y., Zhang, L., Bian, J., Hao, D., Wang, C. Y., et al. (2019) · Science Advances · doi:10.1126/sciadv.aav9075 · Cited by 127 · see citing works
Printability and Physicochemical Properties of Microalgae-Enriched 3D-Printed Snacks
Uribe-Wandurraga, Z. N., Zhang, L., Noort, M. W. J., Schutyser, M. A. I., García‐Segovia, P., & Martı́nez-Monzó, J. (2020) · Food and Bioprocess Technology · doi:10.1007/s11947-020-02544-4 · Cited by 121 · see citing works
Cao, K., Cui, Y., Sun, F., Zhang, H., Fan, J., Ge, B., Cao, Y., Wang, X., Zhu, X., Wei, Z., et al. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108236 · Cited by 112 · see citing works
Symbiotic Photosynthetic Oxygenation within 3D-Bioprinted Vascularized Tissues
Maharjan, S., Alva, J., Cámara, C., Rubio, A. G., Hernández, D., Delavaux, C., Corrêa, É., Romo, M. D., Bonilla, D., Santiago, M. L., et al. (2020) · Matter · doi:10.1016/j.matt.2020.10.022 · Cited by 110 · see citing works
Functionalized Bioink with Optical Sensor Nanoparticles for O2 Imaging in 3D‐Bioprinted Constructs
Trampe, E., Koren, K., Akkineni, A. R., Senwitz, C., Krujatz, F., Lode, A., Gelinsky, M., & Kühl, M. (2018) · Advanced Functional Materials · doi:10.1002/adfm.201804411 · Cited by 104 · see citing works
Bioprinting of Regenerative Photosynthetic Living Materials
Balasubramanian, S., Yu, K., Meyer, A. S., Karana, E., & Aubin‐Tam, M. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202011162 · Cited by 102 · see citing works
Potential application of biomineralization by Synechococcus PCC8806 for concrete restoration
Zhu, T., Paulo, C., Merroun, M. L., & Dittrich, M. (2015) · Ecological Engineering · doi:10.1016/j.ecoleng.2015.05.017 · Cited by 97 · see citing works
Guo, L., Zhang, Y., & Li, W. (2017) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2017.01.003 · Cited by 96 · see citing works
Brar, A., Kumar, M., Soni, T., Vivekanand, V., & Pareek, N. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.125597 · Cited by 96 · see citing works
Bioprinting microporous functional living materials from protein-based core-shell microgels
Ou, Y., Cao, S., Zhang, Y., Zhu, H., Guo, C., Yan, W., Xin, F., Dong, W., Zhang, Y., Narita, M., et al. (2023) · Nature Communications · doi:10.1038/s41467-022-35140-5 · Cited by 96 · see citing works
Review of Low-Cost 3D Bioprinters: State of the Market and Observed Future Trends
Tong, A., Pham, Q. L., Abatemarco, P., Mathew, A., Gupta, D., Iyer, S., & Voronov, R. S. (2021) · SLAS TECHNOLOGY · doi:10.1177/24726303211020297 · Cited by 95 · see citing works
Macro algae Gracilaria verrucosa as a biosorbent: A study of sorption mechanisms
Ata, A., Nalcaci, O. O., & Övez, B. (2012) · Algal Research · doi:10.1016/j.algal.2012.07.001 · Cited by 94 · see citing works
Surface Engineered Biomimetic Inks Based on UV Cross-Linkable Wood Biopolymers for 3D Printing
Xu, W., Zhang, X., Yang, P., et al. (2019) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.9b03442 · Cited by 94 · see citing works
Centeno-Cerdas, C., Jarquín-Cordero, M., Chávez, M. N., Hopfner, Ú., Holmes, C., Schmauß, D., Machens, H., Nickelsen, J., & Egaña, J. T. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.09.060 · Cited by 91 · see citing works
Krujatz, F., Lode, A., Brüggemeier, S., Schütz, K., Kramer, J., Bley, T., Gelinsky, M., & Weber, J. (2015) · Engineering in Life Sciences · doi:10.1002/elsc.201400131 · Cited by 87 · see citing works
González-Camejo, J., Viruela, A., Ruano, M. V., Barat, R., Seco, A., & Ferrer, J. (2019) · Algal Research · doi:10.1016/j.algal.2019.101511 · Cited by 86 · see citing works
Effect of Microalgae (Arthrospira platensis and Chlorella vulgaris) Addition on 3D Printed Cookies
Uribe-Wandurraga, Z. N., Igual, M., Reino-Moyón, J., García‐Segovia, P., & Martı́nez-Monzó, J. (2020) · Food Biophysics · doi:10.1007/s11483-020-09642-y · Cited by 85 · see citing works
4D Cell‐Condensate Bioprinting
Ding, A., Lee, S. J., Tang, R., et al. (2022) · Small · doi:10.1002/smll.202202196 · Cited by 85 · see citing works
Phenotypically complex living materials containing engineered cyanobacteria
Datta, D., Weiss, E. L., Wangpraseurt, D., Hild, E., Chen, S., Golden, J. W., Golden, S. S., & Pokorski, J. K. (2023) · Nature Communications · doi:10.1038/s41467-023-40265-2 · Cited by 84 · see citing works
Haraguchi, Y., Kagawa, Y., Sakaguchi, K., Matsuura, K., Shimizu, T., & Okano, T. (2017) · Scientific Reports · doi:10.1038/srep41594 · Cited by 75 · see citing works
Robotic Extrusion of Algae‐Laden Hydrogels for Large‐Scale Applications
Malik, S., Hagopian, J., Mohite, S., Lintong, C., Stoffels, L., Giannakopoulos, S., Beckett, R., Leung, C., Ruiz, J. G., Cruz, M., et al. (2019) · Global Challenges · doi:10.1002/gch2.201900064 · Cited by 74 · see citing works
Hong, S., Song, S.-J., Lee, J. Y., Jang, H., Choi, J., Sun, K., & Park, Y. (2013) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2013.02.011 · Cited by 73 · see citing works
Biomineralized Biohybrid Algae for Tumor Hypoxia Modulation and Cascade Radio-Photodynamic Therapy
Li, W., Zhong, D., Hua, S., Du, Z. H., & Zhou, M. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c14400 · Cited by 72 · see citing works
Xia, X., Xu, X., Lin, C., Yang, Y., Zeng, L., Zheng, Y. G., Wu, X., Li, W., Xiao, L., Qian, Q., et al. (2020) · ES Materials & Manufacturing · doi:10.30919/esmm5f706 · Cited by 70 · see citing works
Biophotovoltaics: Recent advances and perspectives
Zhu, H., Wang, H., Zhang, Y., & Li, Y. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108101 · Cited by 68 · see citing works
Engineering living building materials for enhanced bacterial viability and mechanical properties
Qiu, J., Artier, J., Cook, S. M., et al. (2021) · iScience · doi:10.1016/j.isci.2021.102083 · Cited by 67 · see citing works
New Trends in Biotechnological Applications of Photosynthetic Microorganisms
Dawiec-Liśniewska, A., Podstawczyk, D., Bastrzyk, A., Czuba, K., Pacyna-Iwanicka, K., Okoro, O. V., & Shavandi, A. (2022) · Biotechnology Advances · doi:10.1016/j.biotechadv.2022.107988 · Cited by 66 · see citing works
Caldwell, G. S., In-na, P., Hart, R., Sharp, E. B., Stefanova, A., Pickersgill, M., Walker, M., Unthank, M. G., Perry, J. J. B., & Lee, J. G. M. (2021) · Energies · doi:10.3390/en14092566 · Cited by 65 · see citing works
Wang, J., Cui, Z., & Maniruzzaman, M. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.123020 · Cited by 64 · see citing works
Translational Aspects of 3D and 4D Printing and Bioprinting
Taylor, S., Mueller, E., Jones, L. R., Makela, A. V., & Ashammakhi, N. A. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202400463 · Cited by 64 · see citing works
3D Printing of Functional Microalgal Silk Structures for Environmental Applications
Zhao, S., Guo, C., Kumarasena, A., Omenetto, F. G., & Kaplan, D. L. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00554 · Cited by 63 · see citing works
Biomimetic 3D living materials powered by microorganisms
Wangpraseurt, D., You, S., Sun, Y., & Chen, S. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.01.003 · Cited by 63 · see citing works
3D Printed Biocatalytic Living Materials with Dual‐Network Reinforced Bioinks
He, F., Ou, Y., Liu, J., Huang, Q. H., Tang, B., Xin, F., Zhang, J., Jiang, M., Chen, S., & Yu, Z. (2021) · Small · doi:10.1002/smll.202104820 · Cited by 61 · see citing works
Zhang, Z., Wu, C., Dai, C., Shi, Q., Fang, G., Xie, D., Zhao, X., Liu, Y., Wang, C. C. L., & Wang, X. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.02.009 · Cited by 60 · see citing works
Cyanobacteria and Microalgae as Sources of Functional Foods to Improve Human General and Oral Health
Ferrazzano, G. F., Papa, C., Pollio, A., Ingenito, A., Sangianantoni, G., & Cantile, T. (2020) · Molecules · doi:10.3390/molecules25215164 · Cited by 56 · see citing works
Jiang, M., Zheng, J., Tang, Y. D., Liu, H., Yao, Y., Zhou, J., Lin, W. J., Ma, Y., Liu, J., & Zhou, J. (2025) · Nature Communications · doi:10.1038/s41467-025-58415-z · Cited by 56 · see citing works
Bacterial Nanobionics via 3D Printing
Joshi, S., Cook, E., & Mannoor, M. S. (2018) · Nano Letters · doi:10.1021/acs.nanolett.8b02642 · Cited by 54 · see citing works
Dual carbon sequestration with photosynthetic living materials
Dranseike, D., Cui, Y., Ling, A. S., Donat, F., Bernhard, S., Bernero, M., Areeckal, A., Lazic, M., Qin, X., Oakey, J. S., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-58761-y · Cited by 54 · see citing works
Cui, J., Sun, H., Chen, R., Sun, J., Mo, G., Luan, G., & Lü, X. (2023) · Green Carbon · doi:10.1016/j.greenca.2023.11.004 · Cited by 48 · see citing works
Self-citationWang, M., Lu, X., Xing, Z., Wei, L., Wang, L., Qian, Y., & Zeng, M. (2023) · Food Research International · doi:10.1016/j.foodres.2023.112823 · Cited by 47 · see citing works
Self-citationKrujatz, F., Dani, S., Windisch, J., Emmermacher, J., Hahn, F., Mosshammer, M., Murthy, S., Steingroewer, J., Walther, T., Kühl, M., et al. (2022) · Biotechnology Advances · doi:10.1016/j.biotechadv.2022.107930 · Cited by 46 · see citing works
Reinhardt, O., Ihmann, S., Ahlhelm, M., & Gelinsky, M. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1145177 · Cited by 46 · see citing works
Kwak, C., Ryu, S. Y., Park, H., et al. (2020) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2020.08.007 · Cited by 43 · see citing works
3D Printed Laminated CaCO3-Nanocellulose Films as Controlled-Release 5-Fluorouracil
Mohan, D., Khairullah, N. F., How, Y. P., et al. (2020) · Polymers · doi:10.3390/polym12040986 · Cited by 41 · see citing works
Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials
Goodchild-Michelman, I. M., Church, G. M., Schubert, M. G., & Tang, T.-C. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100583 · Cited by 41 · see citing works
Windisch, J., Reinhardt, O., Duin, S., Schütz, K., Rodriguez, N. J. N., Liu, S., Lode, A., & Gelinsky, M. (2023) · Advanced Healthcare Materials · doi:10.1002/adhm.202300436 · Cited by 41 · see citing works
Growth, Distribution, and Photosynthesis of Chlamydomonas Reinhardtii in 3D Hydrogels
Oh, J., Ammu, S. K., Vriend, V. D., et al. (2023) · Advanced Materials · doi:10.1002/adma.202305505 · Cited by 41 · see citing works
Review: 3D printing hydrogels for the fabrication of soilless cultivation substrates
Kalossaka, L. M., Sena, G., Barter, L. M. C., & Myant, C. (2021) · Applied Materials Today · doi:10.1016/j.apmt.2021.101088 · Cited by 38 · see citing works
Pagnanelli, F., Jbari, N., Trabucco, F., Martínez, M. E., Sánchez, S., & Toro, L. (2013) · Chemical Engineering Journal · doi:10.1016/j.cej.2013.07.013 · Cited by 34 · see citing works
Bioprinted Living Coral Microenvironments Mimicking Coral‐Algal Symbiosis
Wangpraseurt, D., Sun, Y., You, S., et al. (2022) · Advanced Functional Materials · doi:10.1002/adfm.202202273 · Cited by 34 · see citing works
Zhang, H., Hua, S., He, C., et al. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202404488 · Cited by 34 · see citing works
Printing Green: Microalgae‐Based Materials for 3D Printing with Light
Vazquez‐Martel, C., Martins, L. F., Genthner, E., Almeida, C., Quintana, A. M., Bastmeyer, M., Gómez‐Pinchetti, J. L., & Blasco, E. (2024) · Advanced Materials · doi:10.1002/adma.202402786 · Cited by 31 · see citing works
Giraldo, N. D., Bayona, K. C. D., & Garcés, L. A. (2018) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2018.02.006 · Cited by 30 · see citing works
Spirulina‐based composites for 3D ‐printing
Fredricks, J. L., Iyer, H., McDonald, R., Hsu, J., Jimenez, A. M., & Roumeli, E. (2021) · Journal of Polymer Science · doi:10.1002/pol.20210683 · Cited by 30 · see citing works
Space‐Efficient 3D Microalgae Farming with Optimized Resource Utilization for Regenerative Food
Liu, H., Yu, S., Liu, B., Xiang, S., Jiang, M., Yang, F., Tan, W., Zhou, J., Xiao, M., Li, X., et al. (2024) · Advanced Materials · doi:10.1002/adma.202401172 · Cited by 29 · see citing works
Growth of Synechococcus sp. immobilized in chitosan with different times of contact with NaOH
Aguilar-May, B., Sánchez‐Saavedra, M. D. P., Lizardi‐Mendoza, J., et al. (2006) · Journal of Applied Phycology · doi:10.1007/s10811-006-9132-9 · Cited by 28 · see citing works
Dani, S., Windisch, J., Guerrero, X. M. V., et al. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.994134 · Cited by 26 · see citing works
Rao, S. R., Tripathi, U., & Ravishankar, G. A. (1999) · World Journal of Microbiology and Biotechnology · doi:10.1023/a:1008975901642 · Cited by 25 · see citing works
Algal protein-based 3D-printed fish-analogs as a new approach for sustainable seafood
Alasibi, S., Kazir, M., Israel, A. A., & Livney, Y. D. (2024) · Current Research in Food Science · doi:10.1016/j.crfs.2024.100905 · Cited by 24 · see citing works
The Second International Workshop on Bioprinting, Biopatterning and Bioassembly
Mironov, V. (2005) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.5.8.1111 · Cited by 22 · see citing works
Study on linear bio-structure print process based on alginate bio-ink in 3D bio-fabrication
Gong, Y., Bi, Z., Bian, X., et al. (2020) · Bio-Design and Manufacturing · doi:10.1007/s42242-020-00065-9 · Cited by 21 · see citing works
3D Bioprinting to Fabricate Living Microalgal Materials
Kumar, V., Vlaskin, M. S., & Grigorenko, A. V. (2021) · Trends in biotechnology · doi:10.1016/j.tibtech.2021.10.006 · Cited by 21 · see citing works
Mimicry of the plant leaf with a living hydrogel sheet of cellulose nanofibers
Lee, H., Shin, D., Choi, J., Ki, C. S., & Hyun, J. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119485 · Cited by 21 · see citing works
Glycerol photoproduction by free and Ca-alginate entrapped cells of Chlamydomonas reinhardtii
León, R., & Galván, F. (1995) · Journal of Biotechnology · doi:10.1016/0168-1656(95)00069-3 · Cited by 18 · see citing works
Liu, H., Mei, H., Jiang, H., et al. (2024) · Small · doi:10.1002/smll.202407105 · Cited by 18 · see citing works
A Three-Dimensional Model of Bacterial Biofilms and Its Use in Antimicrobial Susceptibility Testing
Ali, H. R. K., Collier, P., & Bayston, R. (2024) · Microorganisms · doi:10.3390/microorganisms12010203 · Cited by 16 · see citing works
Tóth, G. S., Backman, O., Siivola, T., Xu, W., Kosourov, S. N., Siitonen, V., Xu, C., & Allahverdiyeva, Y. (2024) · Green Chemistry · doi:10.1039/d3gc04264b · Cited by 15 · see citing works
Sun, Z., Wen, H., Di, Z., et al. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.3c00884 · Cited by 11 · see citing works
Diclofenac Degradation by Immobilized Chlamydomonas reinhardtii and Scenedesmus obliquus
Kariyawasam, T., Petkovich, M. P., & Vriens, B. (2024) · MicrobiologyOpen · doi:10.1002/mbo3.70013 · Cited by 11 · see citing works
Liu, L., Gao, Y., Lee, S.-J., et al. (2018) · doi:10.1109/embc.2018.8513490 · Cited by 10 · see citing works
Hendrijantini, N., Sitalaksmi, R. M., Ari, M. D. A., et al. (2020) · Bali Medical Journal · doi:10.15562/bmj.v9i1.1625 · Cited by 10 · see citing works
Impact of 3D printing materials on microalga Chlorella vulgaris
Pozzobon, V., Otaola, F., Arnoudts, C., & Lagirarde, J. (2023) · Bioresource Technology · doi:10.1016/j.biortech.2023.129807 · Cited by 9 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Microalgae biotechnology and its role in sustainable and healthy food design
Schneider, A. T., Machado, R. L. S., Dutra, D. A., Machado, E. F., Dias, R. R., Deprá, M. C., Zepka, L. Q., & Jacob-Lopes, E. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1716473 · Cited by 15 · see citing works
- Authors
- Tang, J., Sun, J., Cui, J., Yuan, X., Luan, G., & Lu, X.
- Affiliations
- Chinese Academy of Sciences (CN)
- Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
- Corresponding author
- Luan, Guodong
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2025-08-27
- Volume
- 23
- Issue
- 9
- Pages
- 342
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC12471347
- OpenAlex
- W4413771421
- Cited by
- 8
- References
- 122
- Citations by year
- 2026: 6 · 2025: 2
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Key Research and Development Program of China (2021YFA0909700, 32270103, 32271484)
- National Natural Science Foundation of China (2021YFA0909700, 32270103, 32271484)
- Shandong Taishan Scholarship (2021YFA0909700, 32270103, 32271484)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Automotive Engineering › Additive Manufacturing and 3D Printing Technologies
- Keywords
- microalgae, 3D bioprinting, biophotovoltaics, bioremediation, tissue engineering, food engineering, microalgal biotechnology, synthetic biology, microalgae cultivation
- MeSH terms
- Biotechnology, Humans, Photosynthesis, Tissue Engineering, Microalgae, Synthetic Biology, Bioprinting, Printing, Three-Dimensional
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
