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Cited by 64Extracellular vesicles in diseaseMetabolomics and Mass Spectrometry StudiesLipid metabolism and biosynthesis

Lipid Profile Characterization and Lipoprotein Comparison of Extracellular Vesicles from Human Plasma and Serum

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Sun, Yuchen, Saito, Kosuke, Saito, Yoshiro·2019·Metabolites
DOI 10.3390/metabo9110259PMC PMC6918450
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Abstract

Extracellular vesicles (EVs) consist of lipid bilayers, occur in various biofluids, and are invaluable in biomarker screening. Liquid chromatography coupled with high-resolution mass spectrometry (LC-MS) was recently used to study comprehensive EV lipid profiles in vitro. The aim of this study was to establish a lipidomics platform for human plasma and serum EVs for comprehensive characterization of their lipid profiles, and to compare them with those of other lipid-containing particles, such as high-density lipoproteins (HDL), and low/very low-density lipoproteins (LDL/VLDL). Isolation was validated by specific protein markers; CD9 and MHC class I for EVs, apoA-I for HDL, and apoB-100 for LDL/VLDL. Lipidomics identified 264 lipids from isolated plasma EVs, HDL, and LDL/VLDL. The absolute lipid levels per unit protein content in the EVs were more than eight times lower than those of the lipoproteins. Moreover, the EVs had higher lysoglycerophospholipid levels than HDL or LDL/VLDL. Similar profiles were also determined for human serum. The present study found that the lipid profiles of EVs are unique and distinctly different from those of lipoproteins. The lipidomics platform applied to human plasma and serum EVs could generate important information for the exploration and qualification of biomarkers in disease diagnosis.

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  • APOE3Ch carriers showed increased enrichment in neutral lipids, likely cholesterol esters.

    “In our study, APOE3Ch carriers showed increased enriched in neutral lipids, likely cholesterol esters, abundant in both lipoproteins and EVs. 59”

    From Plasma extracellular vesicles from <i>APOE3</i> Christchurch carriers display a protective phenotype in early stages of autosomal dominant Alzheimer's disease (2026) · cites this paper as [59]

    The Results (§2.2; page unavailable) identify cholesterol esters as neutral lipids detected in the study of EVs and lipoproteins. They do not establish that cholesterol esters are abundant in both: levels in EVs were over 20 times lower than in lipoproteins, and neutral lipids were excluded from statistical analyses. The APOE3Ch-carrier finding is presented as the citing authors’ own result, not a finding attributed to this source. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Sun, Y., Saito, K., & Saito, Y.
Affiliations
National Institute of Health Sciences (JP)
Corresponding author
Saito, Kosuke
ORCID
Venue
Metabolites
Publisher
MDPI AG
Published
2019-11-01
Volume
9
Issue
11
Pages
259
ISSN
2218-1989 (ISSN-L)
DOI
10.3390/metabo9110259
PMC
PMC6918450
OpenAlex
W2983574246
Cited by
64
References
1
Citations by year
2026: 7 · 2025: 2 · 2024: 17 · 2023: 11 · 2022: 13 · 2021: 6 · 2020: 8
FWCI
2.23
Type
journal article
Language
en
Open access
Licence
Locations
  • Metabolites (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Japan Agency for Medical Research and Development (19ak0101043j0605, 19dk0207043j0602, 19km0405201j0504, 19ek0109278j0103)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
extracellular vesicles, lipidomics, plasma vesicles, lipoproteins, high-density lipoprotein, low-density lipoprotein, VLDL, lipid profile, biomarkers, vesicle isolation, CD9, apolipoprotein A-I, apolipoprotein B-100

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