High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources
Open accessAbstract
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs). We identified 3,532 proteins and 1,961 lipid species in the screen. Exosomes differed from MVs in several different areas: (a) The protein patterns of exosomes were more likely different from their cells of origin than were the protein patterns of MVs; (b) The proteomes of U87 and Huh7 exosomes were similar to each other but different from the proteomes of MSC exosomes, whereas the lipidomes of Huh7 and MSC exosomes were similar to each other but different from the lipidomes of U87 exosomes; (c) exosomes exhibited proteins of extracellular matrix, heparin-binding, receptors, immune response and cell adhesion functions, whereas MVs were enriched in endoplasmic reticulum, proteasome and mitochondrial proteins. Exosomes and MVs also differed in their types of lipid contents. Enrichment in glycolipids and free fatty acids characterized exosomes, whereas enrichment in ceramides and sphingomyelins characterized MVs. Furthermore, Huh7 and MSC exosomes were specifically enriched in cardiolipins; U87 exosomes were enriched in sphingomyelins. This study comprehensively analyses the protein and lipid composition of exosomes, MVs and source cells in 3 different cell types.
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Lipidomic and proteomic analyses on ELVs and MPs released from three different cell lines indicated that lipidomes of ELVs, MPs, and secreting cells were more similar to each other than their proteomes are.
“Lipidomic and proteomic analyses on ELVs and MPs released from three different cell lines have indicated that lipidomes of ELVs, MP and secreting cells were more similar to each other than their proteomes are (Haraszti et al., 2016).”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Haraszti et al., 2016
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- Authors
- Haraszti, R. A., Didiot, M., Sapp, E., Leszyk, J., Shaffer, S. A., Rockwell, H. E., Gao, F., Narain, N. R., DiFiglia, M., Kiebish, M. A., Aronin, N., & Khvorova, A.
- Affiliations
- RNA Therapeutics Institute University of Massachusetts Medical School Worcester MA USA
- Massachusetts General Hospital (US)
- University of Massachusetts Chan Medical School (US)
- Berg (United States) (US)
- Corresponding author
- Khvorova, Anastasia
- ORCID
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2016-01
- Volume
- 5
- Issue
- 1
- Pages
- 32570-32570
- ISSN
- 2001-3078 (ISSN-L)
- PMC
- PMC5116062
- OpenAlex
- W2553886049
- Cited by
- 746
- References
- 55
- Citations by year
- 2026: 54 · 2025: 77 · 2024: 96 · 2023: 70 · 2022: 91 · 2021: 113 · 2020: 84 · 2019: 75 · 2018: 54 · 2017: 31
- FWCI
- 27.88
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Extracellular Vesicles (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- eScholarship@UMassChan (submittedVersion, cc-by-nc)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health (TR 000‐888, GM108803)
- CHDI Foundation (A‐6119)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- exosomes, microvesicles, extracellular vesicles, vesicle proteome, cell of origin, U87 glioblastoma cells, mesenchymal stem cells, cardiolipin, sphingomyelin, ceramide, glycolipids, free fatty acids, extracellular matrix proteins, mitochondrial proteins
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