The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Inside the Black Box: What Makes SELEX Better?
Open accessAbstract
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications. Aptamers are isolated from large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and costly method, and the results are not always successful. Each step in the aptamer selection protocol can influence its results. This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection.
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Cites
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery
Gold, L., Ayers, D., Bertino, J., Bock, C., Bock, A., Brody, E. N., Carter, J., Dalby, A. B., Eaton, B. E., Fitzwater, T., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0015004 · Cited by 1,754
Amplification of complex gene libraries by emulsion PCR
Williams, R., Peisajovich, S. G., Miller, O. J., Magdassi, S., Tawfik, D. S., & Griffiths, A. D. (2006) · Nature Methods · doi:10.1038/nmeth896 · Cited by 358
In vitro evolution suggests multiple origins for the hammerhead ribozyme
Salehi-Ashtiani, K., & Szostak, J. W. (2001) · Nature · doi:10.1038/35102081 · Cited by 183
Ruff, K. M., Snyder, T. M., & Liu, D. R. (2010) · Journal of the American Chemical Society · doi:10.1021/ja103023m · Cited by 86
Size, constant sequences, and optimal selection
LEGIEWICZ, M., LOZUPONE, C., KNIGHT, R., & YARUS, M. (2005) · RNA · doi:10.1261/rna.2161305 · Cited by 69
Cited by
SELEX: Critical factors and optimization strategies for successful aptamer selection
Kohlberger, M., & Gadermaier, G. (2022) · Biotechnology and Applied Biochemistry · doi:10.1002/bab.2244 · Cited by 341
Metadata
- Authors
- Komarova, N., & Kuznetsov, A.
- Venue
- Molecules
- Publisher
- MDPI AG
- Published
- 2019-10-07
- Volume
- 24
- Issue
- 19
- Pages
- 3598
- ISSN
- 1420-3049 (ISSN-L)
- OpenAlex
- W2978945452
- Cited by
- 181
- References
- 6
- FWCI
- 6.02
- Type
- journal article
- Language
- en
- Open access
- gold
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