Aptamers: A Review of Their Chemical Properties and Modifications for Therapeutic Application

Mfold web server for nucleic acid folding and hybridization prediction

Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404

Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

Tuerk, C., & Gold, L. (1990) · Science · doi:10.1126/science.2200121 · Cited by 10,421

In vitro selection of RNA molecules that bind specific ligands

Ellington, A. D., & Szostak, J. W. (1990) · Nature · doi:10.1038/346818a0 · Cited by 9,845

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825 · cited 2×

Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs

Soutschek, J., Akinc, A., Bramlage, B., Charissé, K., Constien, R., Donoghue, M., Elbashir, S. M., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,206

Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery

Gold, L., Ayers, D., Bertino, J., Bock, C., Bock, A., Brody, E. N., Carter, J., Dalby, A. B., Eaton, B. E., Fitzwater, T., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0015004 · Cited by 1,754

Pegaptanib, a targeted anti-VEGF aptamer for ocular vascular disease

Ng, E. W. M., Shima, D. T., Calias, P., Cunningham, E. T., Guyer, D. R., & Adamis, A. P. (2006) · Nature Reviews Drug Discovery · doi:10.1038/nrd1955 · Cited by 1,629

Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution.

Deisenhofer, J. (1981) · Biochemistry · doi:10.1021/bi00512a001 · Cited by 1,539

Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution.

Deisenhofer, J. (1981) · PubMed · Cited by 1,422

2′-Fluoropyrimidine RNA-based Aptamers to the 165-Amino Acid Form of Vascular Endothelial Growth Factor (VEGF165)

Ruckman, J., Green, L. S., Beeson, J., Waugh, S. M., Gillette, W. L., Henninger, D. D., Claesson, L., & Janjić, N. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.32.20556 · Cited by 759

Synthetic genetic polymers capable of heredity and evolution

Pinheiro, V. B., Taylor, A. I., Cozens, C., Abramov, M., Renders, M., Zhang, S., Chaput, J. C., Wengel, J., Peak-Chew, S.-Y., McLaughlin, S. H., et al. (2012) · Science · doi:10.1126/science.1217622 · Cited by 730

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

Kimoto, M., Yamashige, R., Matsunaga, K., Yokoyama, S., & Hirao, I. (2013) · Nature Biotechnology · doi:10.1038/nbt.2556 · Cited by 511

Albumin as a versatile platform for drug half-life extension

Sleep, D., Cameron, J., & Evans, L. R. (2013) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.04.023 · Cited by 510

Hachimoji DNA and RNA: A genetic system with eight building blocks.

Hoshika, S., Leal, N. A., Kim, M.-J., Kim, M.-S., Karalkar, N. B., Kim, H.-J., Bates, A. M., Watkins, N. E., SantaLucia, H. A., Meyer, A. J., et al. (2019) · Science · doi:10.1126/science.aat0971 · Cited by 494

Mirror-image RNA that binds D-adenosine

Klußmann, S., Nolte, A., Bald, R., Erdmann, V. A., & Fürste, J. P. (1996) · Nature Biotechnology · doi:10.1038/nbt0996-1112 · Cited by 397

A New Paradigm for Aptamer Therapeutic AS1411 Action: Uptake by Macropinocytosis and Its Stimulation by a Nucleolin-Dependent Mechanism

Reyes‐Reyes, E. M., Teng, Y., & Bates, P. J. (2010) · Cancer Research · doi:10.1158/0008-5472.can-10-0920 · Cited by 395

Modified deoxyoligonucleotides stable to exonuclease degradation in serum

Shaw, J., Kent, K. M., Bird, J., Fishback, J. A., & Froehler, B. C. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.4.747 · Cited by 370

Characterization of fully 2'-modified oligoribonucleotide hetero- and homoduplex hybridization and nuclease sensitivity

Cummins, L. L., Owens, S. R., Risen, L. M., Lesnik, E. A., Freier, S., McGee, D. P. C., Guinosso, C. J., & Cook, P. D. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.11.2019 · Cited by 364

Direct In Vitro Selection of a 2′-O-Methyl Aptamer to VEGF

Burmeister, P. E., Lewis, S. D., Silva, R. F., Preiss, J. R., Horwitz, L. R., Pendergrast, P. S., McCauley, T. G., Kurz, J. C., Epstein, D. M., Wilson, C., et al. (2005) · Chemistry & Biology · doi:10.1016/j.chembiol.2004.10.017 · Cited by 346

CENTROIDFOLD: a web server for RNA secondary structure prediction

Sato, K., Hamada, M., Asai, K., & Mitsuyama, T. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp367 · Cited by 334

In vitro selection with artificial expanded genetic information systems

Sefah, K., Yang, Z., Bradley, K. M., Hoshika, S., Jiménez, E., Zhang, L., Zhu, G., Shanker, S., Yu, F., Turek, D., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1311778111 · Cited by 330

A mutant T7 RNA polymerase as a DNA polymerase.

Sousa, R., & Padilla, R. M. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb00140.x · Cited by 288

A tripeptide ‘anticodon’ deciphers stop codons in messenger RNA

Ito, K., Uno, M., & Nakamura, Y. (2000) · Nature · doi:10.1038/35001115 · Cited by 284

Sheddable Ternary Nanoparticles for Tumor Acidity-Targeted siRNA Delivery

Yang, X., Du, J., Dou, S., Mao, C., Long, H., & Wang, J. (2011) · ACS Nano · doi:10.1021/nn204240b · Cited by 283

FASTAptamer: A Bioinformatic Toolkit for High-throughput Sequence Analysis of Combinatorial Selections

Alam, K. K., Chang, J. L., & Burke, D. H. (2015) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2015.4 · Cited by 264

Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets

Davies, D. R., Gelinas, A. D., Zhang, C., Rohloff, J. C., Carter, J. D., O’Connell, D., Waugh, S. M., Wolk, S. K., Mayfield, W. S., Burgin, A. B., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1213933109 · Cited by 257

The Aptamer Handbook: Functional Oligonucleotides and Their Applications

(2006) · doi:10.1002/3527608192 · Cited by 220

Selection of DNA Aptamers That Recognize α-Synuclein Oligomers Using a Competitive Screening Method

Tsukakoshi, K., Abe, K., Sode, K., & Ikebukuro, K. (2012) · Analytical Chemistry · doi:10.1021/ac300330g · Cited by 211

A synthetic molecular system capable of mirror-image genetic replication and transcription

Wang, Z., Xu, W., Liu, L., & Zhu, T. F. (2016) · Nature Chemistry · doi:10.1038/nchem.2517 · Cited by 209

Mirror-design of L-oligonucleotide ligands binding to L-arginine

Nolte, A., Klußmann, S., Bald, R., Erdmann, V. A., & Fürste, J. P. (1996) · Nature Biotechnology · doi:10.1038/nbt0996-1116 · Cited by 206

Embracing proteins: structural themes in aptamer–protein complexes

Gelinas, A. D., Davies, D. R., & Janjić, N. (2016) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2016.01.009 · Cited by 206

Characterization of 2′-Fluoro-RNA Aptamers That Bind Preferentially to Disease-associated Conformations of Prion Protein and Inhibit Conversion

Rhie, A., Kirby, L., Sayer, N. M., Wellesley, R., Disterer, P., Sylvester, I., Gill, A. C., Hope, J., James, W., & Tahiri‐Alaoui, A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305297200 · Cited by 184

Assembling OX40 Aptamers on a Molecular Scaffold to Create a Receptor-Activating Aptamer

Dollins, C. M., Nair, S. K., Boczkowski, D., Lee, J., Layzer, J. M., Gilboa, E., & Sullenger, B. A. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2008.05.016 · Cited by 183

Liposome-Anchored Vascular Endothelial Growth Factor Aptamers

Willis, M. C., Collins, B., Zhang, T., Green, L. S., Sebesta, D. P., Bell, C., Kellogg, E. A., Gill, S. C., Magallanez, A., Knauer, S., et al. (1998) · Bioconjugate Chemistry · doi:10.1021/bc980002x · Cited by 182

Highly specific unnatural base pair systems as a third base pair for PCR amplification

Yamashige, R., Kimoto, M., Takezawa, Y., Sato, A., Mitsui, T., Yokoyama, S., & Hirao, I. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr1068 · Cited by 170

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

Carlson-Stevermer, J., Abdeen, A. A., Kohlenberg, L., Goedland, M., Molugu, K., Lou, M., & Saha, K. (2017) · Nature Communications · doi:10.1038/s41467-017-01875-9 · Cited by 170

Aptamer to ErbB-2/HER2 enhances degradation of the target and inhibits tumorigenic growth

Mahlknecht, G., Maron, R., Mancini, M., Schechter, B., Sela, M., & Yarden, Y. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1302594110 · Cited by 169

Crystal structure of NF-κB (p50) 2 complexed to a high-affinity RNA aptamer

Huang, D.-B., Vu, D., Cassiday, L. A., Zimmerman, J. M., Maher, L. J., & Ghosh, G. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1632011100 · Cited by 167

Crystal structure of an RNA aptamer bound to thrombin

Long, S. B., Long, M. B., White, R. R., & Sullenger, B. A. (2008) · RNA · doi:10.1261/rna.1239308 · Cited by 156

Liposome-Anchored Vascular Endothelial Growth Factor Aptamers

Willis, M. C., Collins, B. T., Zhang, T., Green, L. S., Sebesta, D. P., Bell, C. J., Kellogg, E. A., Gill, S. C., Magallanez, A., Knauer, S., et al. (1998) · Bioconjugate Chemistry · doi:10.1021/bc980200o · Cited by 146

Characterization of the interactions of chemically-modified therapeutic nucleic acids with plasma proteins using a fluorescence polarization assay

Gaus, H. J., Gupta, R., Chappell, A. E., Østergaard, M. E., Swayze, E. E., & Seth, P. P. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky1260 · Cited by 144

Evolution of thermophilic DNA polymerases for the recognition and amplification of C2ʹ-modified DNA

Chen, T., Hongdilokkul, N., Liu, Z., Adhikary, R., Tsuen, S. S., & Romesberg, F. E. (2016) · Nature Chemistry · doi:10.1038/nchem.2493 · Cited by 143

A mutation in T7 RNA polymerase that facilitates promoter clearance

Guillerez, J., Lopez, P. J., Proux, F., Launay, H. M. M., & Dreyfus, M. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407141102 · Cited by 139

Oligonucleotide‐Based Mimetics of Hepatocyte Growth Factor

Ueki, R., Ueki, A., Kanda, N., & Sando, S. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201508572 · Cited by 139

Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: Tailored-SELEX

Vater, A. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng130 · Cited by 135

Stereospecificity of nucleases towards phosphorothioate-substituted RNA: stereochemistry of transcription by T7 RNA polymerase

Griffiths, A. D., POTTER, B. V. L., & Eperon, I. C. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.10.4145 · Cited by 127

The isolation of an RNA aptamer targeting to p53 protein with single amino acid mutation

Chen, L., Rashid, F., Shah, A., Awan, H. M., Wu, M., Liu, A., Wang, J., Zhu, T., Luo, Z., & Shan, G. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1502159112 · Cited by 126

Oligonucleotide duplexes containing 2′-amino-2′-deoxycytidines: thermal stability and chemical reactivity

Aurup, H., Tuschl, T., Benseler, F., Ludwig, J., & Eckstein, F. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.1.20 · Cited by 126

Aptamer-Mediated Delivery of Splice-Switching Oligonucleotides to the Nuclei of Cancer Cells

Kotula, J. W., Pratico, E. D., Ming, X., Nakagawa, O., Juliano, R. L., & Sullenger, B. A. (2012) · Nucleic Acid Therapeutics · doi:10.1089/nat.2012.0347 · Cited by 124

Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G

Miyakawa, S., Nomura, Y., Sakamoto, T., Yamaguchi, Y., Kato, K., Yamazaki, S., & Nakamura, Y. (2008) · RNA · doi:10.1261/rna.1005808 · Cited by 118

Selection and Characterization of an RNA Decoy for Transcription Factor NF-κB

Lebruska, L. L., & Maher, L. J. (1999) · Biochemistry · doi:10.1021/bi982515x · Cited by 117

Anticoagulant characteristics of HD1‐22, a bivalent aptamer that specifically inhibits thrombin and prothrombinase

Müller, J., Freitag, D. F., Mayer, G., & Pötzsch, B. (2008) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2008.03162.x · Cited by 115

CRISPR-mediated Activation of Latent HIV-1 Expression

Limsirichai, P., Gaj, T., & Schaffer, D. V. (2015) · Molecular Therapy · doi:10.1038/mt.2015.213 · Cited by 112

Pharmacokinetics of a Cholesterol-conjugated Aptamer Against the Hepatitis C Virus (HCV) NS5B Protein

Lee, C. H., Lee, S.-H., Kim, J. H., Noh, Y.-H., Noh, G., & Lee, S. (2015) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2015.30 · Cited by 106

Mechanism of Ribose 2‘-Group Discrimination by an RNA Polymerase

Huang, Y., Eckstein, F., Padilla, R. M., & Sousa, R. (1997) · Biochemistry · doi:10.1021/bi962674l · Cited by 104

Conformational plasticity of RNA for target recognition as revealed by the 2.15 Å crystal structure of a human IgG–aptamer complex

Nomura, Y., Sugiyama, S., Sakamoto, T., Miyakawa, S., Adachi, H., Takano, K., Murakami, S., Tsuyoshi Inoue, A., Mori, Y., Nakamura, Y., et al. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq615 · Cited by 103

Specific inhibition of splicing factor activity by decoy RNA oligonucleotides

Denichenko, P., Mogilevsky, M., Cléry, A., Welte, T., Biran, J., Tepper, O., Barnabas, G. D., Danan-Gotthold, M., Kumar, S., Yavin, E., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09523-0 · Cited by 101

New NTP analogs: the synthesis of 4′-thioUTP and 4′-thioCTP and their utility for SELEX

Kato, Y., Minakawa, N., Komatsu, Y., Kamiya, H., Ogawa, N., Harashima, H., & Matsuda, A. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki578 · Cited by 101

Bioinformatic Analysis of the Contribution of Primer Sequences to Aptamer Structures

Cowperthwaite, M. C., & Ellington, A. D. (2008) · Journal of Molecular Evolution · doi:10.1007/s00239-008-9130-4 · Cited by 100

Novel Combinatorial Selection of Phosphorothioate Oligonucleotide Aptamers

King, D. J., Ventura, D. A., Brasier, A. R., & Gorenstein, D. G. (1998) · Biochemistry · doi:10.1021/bi981780f · Cited by 100

Anti-PEG Antibodies Inhibit the Anticoagulant Activity of PEGylated Aptamers

Moreno, A., Pitoc, G. A., Ganson, N. J., Layzer, J. M., Hershfield, M. S., Tarantal, A. F., & Sullenger, B. A. (2019) · Cell chemical biology · doi:10.1016/j.chembiol.2019.02.001 · Cited by 100

Mimicry Grasps Reality in Translation Termination

Nakamura, Y., Ito, K., & Ehrenberg, M. (2000) · Cell · doi:10.1016/s0092-8674(00)80845-4 · Cited by 97

Development of a Bifunctional Aptamer Targeting the Transferrin Receptor and Epithelial Cell Adhesion Molecule (EpCAM) for the Treatment of Brain Cancer Metastases

MacDonald, J. P., Henri, J., Goodman, L., Xiang, D., Duan, W., & Shigdar, S. L. (2016) · ACS Chemical Neuroscience · doi:10.1021/acschemneuro.6b00369 · Cited by 97

In vitro RNA random pools are not structurally diverse: A computational analysis

Gevertz, J. L., Gan, H. H., & Tamar Schlick, A. (2005) · RNA · doi:10.1261/rna.7271405 · Cited by 94

Modeling, synthesis, and hybridization properties of (L)-ribonucleic acid

Ashley, G. W. (1992) · Journal of the American Chemical Society · doi:10.1021/ja00051a001 · Cited by 93

Silencing Myostatin Using Cholesterol-conjugated siRNAs Induces Muscle Growth

Khan, T., Weber, H., DiMuzio, J. M., Matter, A. M., Dogdas, B., Shah, T., Thankappan, A., Disa, J., Jadhav, V., Lubbers, L. S., et al. (2016) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2016.55 · Cited by 90

Direct demonstration of a neonatal Fc receptor (FcRn)-driven endosomal sorting pathway for cellular recycling of albumin

Schmidt, E. G. W., Hvam, M. L., Antunes, F., Cameron, J., Viuff, D., Andersen, B., Kristensen, N. N., & Howard, K. A. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.794248 · Cited by 89

Optimization of enzymatic reaction conditions for generating representative pools of cDNA from small RNA

Db, M., & Gb, R. (2010) · RNA: A publication of the RNA Society · doi:10.1261/rna.2242610 · Cited by 84

A thermostable d-polymerase for mirror-image PCR

Pech, A., Achenbach, J., Jahnz, M., Schülzchen, S., Jarosch, F., Bordusa, F., & Klußmann, S. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx079 · Cited by 83

Immunotherapy of CD30-expressing lymphoma using a highly stable ssDNA aptamer

Parekh, P. A., Kamble, S., Zhao, N., Zeng, Z., Portier, B. P., & Zu, Y. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.07.099 · Cited by 80

Post-SELEX Chemical Optimization of a Trypanosome-Specific RNA Aptamer

Göringer, H. U., Adler, A., Forster, N., & Homann, M. (2008) · Combinatorial Chemistry & High Throughput Screening · doi:10.2174/138620708783398331 · Cited by 80

AptaTRACE Elucidates RNA Sequence-Structure Motifs from Selection Trends in HT-SELEX Experiments

Dao, P., Hoinka, J., Takahashi, M. M., Zhou, J., Ho, M., Wang, Y., Costa, F., Rossi, J. J., Backofen, R., Burnett, J., et al. (2016) · Cell Systems · doi:10.1016/j.cels.2016.07.003 · Cited by 78

Aptamer imaging with Cu-64 labeled AS1411: Preliminary assessment in lung cancer

Li, J., Zheng, H., Bates, P. J., Malik, T., Li, X., Trent, J. O., & Ng, C. K. (2013) · Nuclear Medicine and Biology · doi:10.1016/j.nucmedbio.2013.10.008 · Cited by 77

Transcription yield of fully 2′-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants

Meyer, A., Garry, D., Hall, B., Byrom, M., McDonald, H. G., Yang, X., Yin, Y., & Ellington, A. D. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv734 · Cited by 76

Architecture of high-affinity unnatural-base DNA aptamers toward pharmaceutical applications

Matsunaga, K., Kimoto, M., Hanson, C. A., Sanford, M. E., Young, H. A., & Hirao, I. (2015) · Scientific Reports · doi:10.1038/srep18478 · Cited by 76

Synthesis and radiolabeling of chelator–RNA aptamer bioconjugates with copper-64 for targeted molecular imaging

Rockey, W. M., Huang, L., Kloepping, K. C., Baumhover, N. J., Giangrande, P. H., & Schultz, M. K. (2011) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2011.05.010 · Cited by 75

Boron-containing aptamers to ATP

Lato, S. M. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.6.1401 · Cited by 75

Pegaptanib for the treatment of age-related macular degeneration

Zhou, B., & Wang, B. (2006) · Experimental Eye Research · doi:10.1016/j.exer.2006.02.010 · Cited by 72

Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering siRNAs to Target Cells

Thiel, W. H., Thiel, K. W., Flenker, K. S., Bair, T., Dupuy, A. J., McNamara, J. O., Miller, F. J., & Giangrande, P. H. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-1538-5_11 · Cited by 69

DNA aptamer assemblies as fibroblast growth factor mimics and their application in stem cell culture

Ueki, R., Atsuta, S., Ueki, A., Hoshiyama, J., Li, J., Hayashi, Y., & Sando, S. (2019) · Chemical Communications · doi:10.1039/c8cc08080a · Cited by 69

Synergistic inhibition of lung cancer cell invasion, tumor growth and angiogenesis using aptamer-siRNA chimeras

Lai, W., Wang, W.-Y., Chang, Y.-C., Chang, C.-J., Yang, P., & Peck, K. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2013.12.054 · Cited by 67

In vitro selection of BNA (LNA) aptamers

Kuwahara, M., & Obika, S. (2013) · Artificial DNA PNA & XNA · doi:10.4161/adna.25786 · Cited by 67

Nucleotide Bias Observed with a Short SELEX RNA Aptamer Library

Thiel, W. H., Bair, T., Wyatt Thiel, K., Dassie, J. P., Rockey, W. M., Howell, C. A., Liu, X. Y., Dupuy, A. J., Huang, L., Owczarzy, R., et al. (2011) · Nucleic Acid Therapeutics · doi:10.1089/nat.2011.0288 · Cited by 66

In Vivo SELEX of an Inhibitory NSCLC-Specific RNA Aptamer from PEGylated RNA Library

Wang, H., Zhang, Y., Yang, H., Qin, M., Ding, X., Liu, R., & Jiang, Y. (2017) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2017.12.003 · Cited by 66

Decreasing miRNA sequencing bias using a single adapter and circularization approach

Barberán-Soler, S., Vo, J. M., Hogans, R. E., Dallas, A., Johnston, B. H., & Kazakov, S. A. (2018) · Genome biology · doi:10.1186/s13059-018-1488-z · Cited by 65

In vitro selection of phosphorothiolated aptamers

Jhaveri, S. D., Olwin, B. B., & Ellington, A. D. (1998) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/s0960-894x(98)00414-4 · Cited by 64

Yeast genetic selections to optimize RNA decoys for transcription factor NF-κB

Cassiday, L. A., & Maher, L. J. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0736013100 · Cited by 62

Aptamers as candidate therapeutics for cardiovascular indications

Keefe, A. D., & Schaub, R. G. (2008) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2007.12.005 · Cited by 62

The crystal structure of a high affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: Further challenges in the modeling of ligand–RNA interactions

Horn, W. T., Convery, M. A., Stonehouse, N. J., ADAMS, C. J., Liljas, L., Phillips, S. E. V., & Stockley, P. G. (2004) · RNA · doi:10.1261/rna.7710304 · Cited by 62

In vitro selection using a dual RNA library that allows primerless selection

Jarosch, F. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl463 · Cited by 61

In silico approaches to RNA aptamer design

Hamada, M. (2017) · Biochimie · doi:10.1016/j.biochi.2017.10.005 · Cited by 58

MPBind: a Meta-motif-based statistical framework and pipeline to Predict Binding potential of SELEX-derived aptamers

Jiang, P., MEYER, S. R., Hou, Z., Propson, N. E., Soh, H. T., Thomson, J. A., & Stewart, R. M. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu348 · Cited by 57

Acyclic Identification of Aptamers for Human alpha-Thrombin Using Over-Represented Libraries and Deep Sequencing

Kupakuwana, G. V., Crill, J. E., McPike, M. P., & Borer, P. N. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019395 · Cited by 55

Targeted Disruption of β-Arrestin 2-Mediated Signaling Pathways by Aptamer Chimeras Leads to Inhibition of Leukemic Cell Growth

Kotula, J. W., Sun, J., Li, M., Pratico, E. D., Fereshteh, M., Ahrens, D. P., Sullenger, B. A., & Kovacs, J. J. (2014) · PLoS ONE · doi:10.1371/journal.pone.0093441 · Cited by 51

H1 RNA polymerase III promoter-driven expression of an RNA aptamer leads to high-level inhibition of intracellular protein activity

Mi, J. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl482 · Cited by 51

DNA-Aptamer Targeting Vimentin for Tumor Therapy In Vivo

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Kostyuk, D. A., Dragan, S. M., Lyakhov, D. L., Rechinsky, V. O., Tunitskaya, V. L., CHERNOV, B. K., & Kochetkov, S. N. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)00732-o · Cited by 49

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Skogen, M., Roth, J. A., Yerkes, S., Parekh‐Olmedo, H., & Kmiec, E. B. (2006) · BMC Neuroscience · doi:10.1186/1471-2202-7-65 · Cited by 36

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Schoetzau, T., Langner, J., Moyroud, E., Roehl, I., Vonhoff, S., & Klußmann, S. (2003) · Bioconjugate Chemistry · doi:10.1021/bc0256547 · Cited by 32

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Ouellet, E., Lagally, E. T., Cheung, K. C., & Haynes, C. A. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25294 · Cited by 27

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Mai, J., Li, X., Zhang, G., Huang, Y., Xu, R., Shen, Q., Lokesh, G. L.-R., Thiviyanathan, V., Chen, L., Liu, H., et al. (2018) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.7b01169 · Cited by 17

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Frutiger, A., Tanno, A., Hwu, S., Tiefenauer, R. F., Vörös, J., & Nakatsuka, N. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00044 · Cited by 287

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