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Cited by 46Insect Pest Control StrategiesEssential Oils and Antimicrobial ActivityInsect Pheromone Research and Control

Optimal Extraction of Ocimum basilicum Essential Oil by Association of Ultrasound and Hydrodistillation and Its Potential as a Biopesticide Against a Major Stored Grains Pest

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da Silva Moura, Eridiane, D’Antonino Faroni, Lêda Rita, Fernandes Heleno, Fernanda, Aparecida Zinato Rodrigues, Alessandra, Figueiredo Prates, Lucas Henrique, Lopes Ribeiro de Queiroz, Maria Eliana·2020·Molecules
DOI 10.3390/molecules25122781PMC PMC7355779
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Abstract

The essential oil of basil (Ocimum basilicum) has significant biological activity against insect pests and can be extracted through various techniques. This work aimed to optimize and validate the extraction process of the essential oil of O. basilicum submitted to different drying temperatures of the leaves and extracted by the combination of a Clevenger method and ultrasound. The biological activity of the extracted oil under different conditions was evaluated for potential control of Sitophilus zeamais. The extraction method was optimized according to the sonication time by ultrasound (0, 8, 19, 31 and 38 min) and hydrodistillation (20, 30, 45, 60 and 70 min) and drying temperature (20, 30, 45, 60 and 70 °C). The bioactivity of the essential oil was assessed against adults of S. zeamais and the effects of each variable and its interactions on the mortality of the insects were evaluated. The best yield of essential oil was obtained with the longest sonication and hydrodistillation times and the lowest drying temperature of leaves. Higher toxicity of the essential oil against S. zeamais was obtained by the use of ultrasound for its extraction. The identification and the relative percentage of the compounds of the essential oil were performed with a gas chromatograph coupled to a mass selective detector. The performance of the method was assessed by studying selectivity, linearity, limits of detection (LOD) and quantification (LOQ), precision and accuracy. The LOD and LOQ values for linalool were 2.19 and 6.62 µg mL−1 and for estragole 2.001 and 6.063 µg mL−1, respectively. The coefficients of determination (R2) were >0.99. The average recoveries ranged between 71 and 106%, with coefficient of variation ≤6.4%.

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  • Classification based on volatile oil composition requires distillation and fractionation of oils, and chemotype classification based on only one major volatile oil is erroneous because one plant may contain two or more chemical compounds in nearly equal amounts.

    “Furthermore, classification based on volatile oil composition requires the distillation and fractionation of oils, and the chemotype classification based on only one major volatile oil is erroneous as one plant may contain two or more chemical compounds in nearly equal amounts [15,20,21].”

    From Comprehensive Metabolomic Fingerprinting Combined with Chemometrics Identifies Species- and Variety-Specific Variation of Medicinal Herbs: An Ocimum Study (2023) · cites this paper as [15]

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Research works printed but not linked: 1

  1. [50]Mejri J. Aydib A. Abderrabbab M. Mejric M. Emerging extraction processes of essential oils: A review Asian J. Green Chem. 2015 1 22

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Authors
da Silva Moura, E., D’Antonino Faroni, L. R., Fernandes Heleno, F., Aparecida Zinato Rodrigues, A., Figueiredo Prates, L. H., & Lopes Ribeiro de Queiroz, M. E.
Affiliations
  • Department of Agricultural Engineering, Universidade Federal de Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
  • Department of Chemistry, Universidade Federal de Viçosa, Viçosa 36570-900, Minas Gerais, Brazil
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2020-06-16
Volume
25
Issue
12
Pages
2781
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules25122781
PMC
PMC7355779
OpenAlex
W3034563797
Cited by
46
References
46
Citations by year
2026: 5 · 2025: 6 · 2024: 12 · 2023: 10 · 2022: 4 · 2021: 5 · 2020: 4
FWCI
3.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect Pheromone Research and Control
Keywords
Ocimum basilicum, basil essential oil, Sitophilus zeamais, stored grain pests, biopesticides, insecticidal activity, linalool, estragole, sonication time, GC-MS quantification
MeSH terms
Ultrasonic Waves, Animals, Coleoptera, Oils, Volatile, Pest Control, Biological, Ocimum basilicum, Biological Control Agents

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