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Cited by 10Essential Oils and Antimicrobial ActivityMedicinal Plant ResearchPhytochemistry and Biological Activities

Anti-Inflammatory Effect of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Essential Oil

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Kim, Sung-Hee, Jang, Young-Ah, Kwon, Yong-Jin·2024·Molecules
DOI 10.3390/molecules29051117PMC PMC10933829
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Abstract

Chamaecyparis obtusa (Siebold & Zucc.) Endl. (C. obtusa) belongs to the Cupressaceae family and is native to East Asian regions. Essential oils extracted from the leaves, bark, branches, and roots of C. obtusa have both aesthetic and medicinal properties and are thus widely used. However, detailed analyses of the active ingredients of C. obtusa extract are lacking. In this study, the sabinene content in the hydro-distillation of C. obtusa leaf essential oil (COD) was analyzed using GC-MS, and the anti-inflammatory effect of COD was compared with that of pure sabinene. Cell viability was evaluated by MTT assay, and nitric oxide (NO) production was measured using Griess reagent. Relative mRNA and protein levels were analyzed using RT-qPCR and western blot, and secreted cytokines were analyzed using a cytokine array kit. The results showed that both COD and sabinene inhibited the expression of inducible nitric oxide synthase (iNOS) and the phosphorylation of c-Jun N-terminal kinase (JNK) and p38 in lipopolysaccharide (LPS)-induced RAW 264.7 cells. COD and sabinene also reduced the production of pro-inflammatory cytokines interleukin (IL)-1β, IL-6, IL-27, IL-1 receptor antagonist (IL-1ra), and granulocyte-macrophage colony-stimulating factor (GM-CSF). The anti-inflammatory mechanisms of COD and sabinene partially overlap, as COD was shown to inhibit MAPKs and the JAK/STAT axis, and sabinene inhibited MAPKs, thereby preventing LPS-induced macrophage activation.

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Authors
Kim, S.-H., Jang, Y.-A., & Kwon, Y.-J.
Affiliations
  • Kyungsung University (KR)
  • Daegu Haany University (KR)
Corresponding author
  • Jang, Young-Ah
  • Kwon, Yong-Jin
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2024-03-01
Volume
29
Issue
5
Pages
1117
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules29051117
PMC
PMC10933829
OpenAlex
W4392360016
Cited by
10
References
39
Citations by year
2026: 4 · 2025: 6
FWCI
2.15
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
National Research Foundation of Korea (2022R1F1A1074297)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
Keywords
Chamaecyparis obtusa, leaf essential oil, sabinene, anti-inflammatory activity, inducible nitric oxide synthase, JNK phosphorylation, p38 MAPK, MAPK signaling, JAK/STAT signaling, inflammatory cytokines, macrophage activation, lipopolysaccharide, RAW 264.7 macrophages, Cupressaceae
MeSH terms
Bicyclic Monoterpenes, Anti-Inflammatory Agents, Lipopolysaccharides, Nitric Oxide, Oils, Volatile, Cytokines, Plant Leaves, Chamaecyparis, Nitric Oxide Synthase Type II

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