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Cited by 14Characterization and Applications of Magnetic NanoparticlesNanoparticle-Based Drug DeliveryImmunotoxicology and immune responses

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Biocompatibility of Dextran-Coated 30 nm and 80 nm Sized SPIONs towards Monocytes, Dendritic Cells and Lymphocytes

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Zschiesche, Lisa, Janko, Christina, Friedrich, Bernhard, Frey, Benjamin, Band, Julia, Lyer, Stefan, Alexiou, Christoph, Unterweger, Harald·2022·Nanomaterials
DOI 10.3390/nano13010014PMC PMC9823599
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Abstract

Dextran-coated superparamagnetic iron oxide nanoparticles (SPIONDex) of various sizes can be used as contrast agents in magnetic resonance imaging (MRI) of different tissues, e.g., liver or atherosclerotic plaques, after intravenous injection. In previous studies, the blood compatibility and the absence of immunogenicity of SPIONDex was demonstrated. The investigation of the interference of SPIONDex with stimulated immune cell activation is the aim of this study. For this purpose, sterile and endotoxin-free SPIONDex with different hydrodynamic sizes (30 and 80 nm) were investigated for their effect on monocytes, dendritic cells (DC) and lymphocytes in concentrations up to 200 µg/mL, which would be administered for use as an imaging agent. The cells were analyzed using flow cytometry and brightfield microscopy. We found that SPIONDex were hardly taken up by THP-1 monocytes and did not reduce cell viability. In the presence of SPIONDex, the phagocytosis of zymosan and E. coli by THP-1 was dose-dependently reduced. SPIONDex neither induced the maturation of DCs nor interfered with their stimulated maturation. The particles did not induce lymphocyte proliferation or interfere with lymphocyte proliferation after stimulation. Since SPIONDex rapidly distribute via the blood circulation in vivo, high concentrations were only reached locally at the injection site immediately after application and only for a very limited time. Thus, SPIONDex can be considered immune compatible in doses required for use as an MRI contrast agent.

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  • SPIONs are biocompatible.

    “Moreover, SPIONs are biocompatible [2,6].”

    From Experimental and Simulative Analysis of Magnetic Nanoparticle Accumulation Using Various Halbach Arrays (2024) · cites this paper as [2]

    The Discussion concludes that the tested dextran-coated SPIONs are highly biocompatible at doses relevant for MRI. The citing sentence extends that finding to SPIONs generally, regardless of coating or dose, which this study does not establish. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Metadata

Authors
Zschiesche, L., Janko, C., Friedrich, B., Frey, B., Band, J., Lyer, S., Alexiou, C., & Unterweger, H.
Affiliations
  • Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
  • Universitätsklinikum Erlangen (DE)
Corresponding author
Unterweger, Harald
ORCID
Venue
Nanomaterials
Publisher
MDPI AG
Published
2022-12-20
Volume
13
Issue
1
Pages
14
ISSN
2079-4991 (ISSN-L)
DOI
10.3390/nano13010014
PMC
PMC9823599
OpenAlex
W4313506809
Cited by
14
References
0
Citations by year
2026: 2 · 2025: 3 · 2024: 5 · 2023: 4
FWCI
0.87
Type
journal article
Language
en
Open access
Licence
Locations
  • Nanomaterials (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Manfred Roth Foundation, Fürth, Germany
  • Forschungsstiftung Medizin am Universitätsklinikum Erlangen
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
Keywords
biocompatibility, MRI contrast agents, nanoparticle size effects, monocytes, dendritic cell maturation, lymphocyte proliferation, phagocytosis, THP-1 cells, immune cell activation, nanoparticle toxicity

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