Protein Supplementation Enhances the Effects of Intermittent Loading on Skeletal Muscles by Activating the mTORC1 Signaling Pathway in a Rat Model of Disuse Atrophy
Open accessAbstract
Inactivity leads to skeletal muscle atrophy, whereas intermittent loading (IL) during hind limb unloading (HU) attenuates muscle atrophy. However, the combined effects of IL and protein supplementation on disuse muscle atrophy are unclear. Therefore, we investigated the effects of IL and a high-protein oral nutritional supplement (HP) during HU on skeletal muscle mass and protein synthesis/breakdown. Male F344 rats were assigned to the control (CON), 14-day HU (HU), IL during HU (HU + IL), and IL during HU followed by HP administration (2.6 g protein/kg/day; HU + IL + HP) groups. Soleus and gastrocnemius muscles were sampled 30 min after the last IL and HP supplementation. HU decreased relative soleus and gastrocnemius muscle masses. Relative muscle masses and p70 ribosomal protein S6 kinase/ribosomal protein S6 phosphorylation in soleus and gastrocnemius muscles were higher in the HU + IL group than the HU group and further higher in the HU + IL + HP group than the HU + IL group in gastrocnemius muscle. Therefore, protein administration plus IL effectively prevented skeletal muscle atrophy induced by disuse, potentially via enhanced activation of targets downstream of mammalian target of rapamycin complex 1 (mTORC1) signaling pathway.
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Oral administration of a high protein liquid nutritional supplement (milk protein, whey protein, leucine, and citrulline) inhibits intermittent loading-induced muscle atrophy and increases muscle mass by activating mTORC1 signaling.
“Oral administration of a high protein liquid nutritional supplement (milk protein, whey protein, leucine, and citrulline) inhibits intermittent loading-induced muscle atrophy and increases muscle mass by activating the mTORC1 signaling pathway (Ribosomal protein S6 kinase (p70S6K) and ribosomal protein S6 (rpS6)) [20].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [20]
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Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy
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- Authors
- Miyatake, S., Hino, K., Natsui, Y., Ebisu, G., & Fujita, S.
- Affiliations
- Otsuka (Japan) (JP)
- Ritsumeikan University (JP)
- Corresponding author
- Fujita, Satoshi
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2020-09-07
- Volume
- 12
- Issue
- 9
- Pages
- 2729
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC7551819
- OpenAlex
- W3083349290
- Cited by
- 7
- References
- 44
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2021: 2
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Japan Society for the Promotion of Science (17H02183)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Keywords
- protein supplementation, intermittent loading, disuse atrophy, hindlimb unloading, mTORC1 signaling, skeletal muscle mass, muscle protein synthesis, muscle protein degradation, S6 phosphorylation, soleus muscle, gastrocnemius
- MeSH terms
- Mechanistic Target of Rapamycin Complex 1, Amino Acids, Animals, Dietary Proteins, Disease Models, Animal, Male, Muscular Atrophy, Phosphorylation, Proteins, Rats, Inbred F344, Signal Transduction, Muscle, Skeletal, Hindlimb Suspension, Dietary Supplements, Muscular Disorders, Atrophic, Ribosomal Protein S6 Kinases, 70-kDa, Rats
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