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Cited by 16Parasites and Host InteractionsHelminth infection and controlMollusks and Parasites Studies

Ascaris suum Nutrient Uptake and Metabolic Release, and Modulation of Host Intestinal Nutrient Transport by Excretory-Secretory and Cuticle Antigens In Vitro

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Koehler, Sarina, Springer, Andrea, Issel, Nicole, Klinger, Stefanie, Wendt, Michael, Breves, Gerhard, Strube, Christina·2021·Pathogens
DOI 10.3390/pathogens10111419PMC PMC8618387
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Abstract

Ascaris suum, the most important pig parasite, also infects humans as a zoonotic pathogen. Malabsorption upon infection probably results from impaired nutrient transport, presumably mediated by the parasite’s excretory-secretory (ES) or cuticle somatic (CSO) antigens. The present study investigated the electrogenic transport (ΔIsc) of glucose, alanine and the dipeptide glycyl-l-glutamine (glygln), as well as glucose net flux rates in pig jejunal tissue after in vitro exposure to adult A. suum total ES or CSO antigens in Ussing chambers. ΔIsc of glucose, alanine and glucose net flux rate were significantly decreased after one hour of exposure to total ES antigen. In contrast, CSO antigens increased the transport of glygln. Additionally, nutrient uptake and ES antigen pattern were compared in culture medium from untreated adult worms and those with sealed mouth and anal openings. Untreated worms completely absorbed glucose, while cuticular absorption in sealed worms led to 90% reduction. Amino acid absorption was 30% less effective in sealed worms, and ammonia excretion decreased by 20%. Overall, the results show that A. suum total ES antigen rapidly impairs nutrient transport in vitro. Future studies confirming the results in vivo, narrowing down the ES components responsible and investigating underlying molecular mechanisms are needed.

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Crossref · 10.3390/pathogens10111419

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  • Exposure to roundworm antigens activates the local immune system, specifically Th2 infiltrations and cytokine induction, altering the gut-microbiota balance.

    “Exposure to roundworm antigens also activated the local immune system, specifically Th2 infiltrations and cytokine induction, altering the gut-microbiota balance.”

    From Intestinal Parasitic Infection and Nutritional Status in Children under Five Years Old: A Systematic Review (2022) · cites this paper as [67]

    The Introduction (page unavailable) says A. suum excretory-secretory antigens can modulate host immune responses. It does not report Th2 infiltration, cytokine induction, or a resulting change in gut-microbiota balance; the Conclusions (page unavailable) instead concern nutrient transport after antigen exposure. (LLM, conf=0.84)

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Authors
Koehler, S., Springer, A., Issel, N., Klinger, S., Wendt, M., Breves, G., & Strube, C.
Affiliations
  • University of Veterinary Medicine Hannover, Foundation (DE)
  • Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
ORCID
Venue
Pathogens
Publisher
MDPI AG
Published
2021-11-01
Volume
10
Issue
11
Pages
1419
ISSN
2076-0817 (ISSN-L)
DOI
10.3390/pathogens10111419
PMC
PMC8618387
OpenAlex
W3208420121
Cited by
16
References
43
Citations by year
2026: 4 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 7
FWCI
1.89
Type
journal article
Language
en
Open access
Licence
Locations
  • Pathogens (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Deutsche Forschungsgemeinschaft (STR 1171/14-1 and BR 780/23-1)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Mollusks and Parasites Studies
Keywords
Ascaris suum, excretory-secretory antigens, intestinal nutrient transport, glucose transport, alanine transport, malabsorption, host-parasite interactions, zoonotic nematodes, ammonia excretion, Ussing chamber

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