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Cited by 41Plant Pathogens and Fungal DiseasesFungal and yeast genetics researchPlant Disease Resistance and Genetics

CgSCD1 is essential for melanin biosynthesis and pathogenicity of Colletotrichum gloeosporioides

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Wang, Tan, Ren, Dandan, Guo, Han, Chen, Xue, Zhu, Pinkuan, Nie, Haozhen, Xu, Ling·2020·Pathogens
DOI 10.3390/pathogens9020141PMC PMC7169410
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Abstract

Colletotrichum gloeosporioides, an important phytopathogenic fungus, mainly infects tropical fruits and results in serious anthracnose. Previous studies have shown that melanin biosynthesis inhibitor can inhibit the melanization of the appressoria of Magnaporthe grisea and Colletotrichum orbiculare, resulting in limited infection of the hosts. In this study, we identified and characterized a scytalone dehydratase gene (CgSCD1) from C. gloeosporioides which is involved in melanin synthesis. The CgSCD1 gene deletion mutant ΔCgscd1 was obtained using homologous recombination. The ΔCgscd1 mutant showed no melanin accumulation on appressoria formation and vegetative hyphae. Furthermore, the virulence of ΔCgscd1 was significantly reduced in comparison with the wild-type (WT) strain. Further investigations showed that the growth rate as well as germination and appressorium formation of ΔCgscd1 displayed no difference compared to the wild-type and complemented transformant Cgscd1com strains. Furthermore, we found that the appressorial turgor pressure in the ΔCgscd1 mutant showed no difference compared to that in the WT and Cgscd1com strains in the incipient cytorrhysis experiment. However, fewer infectious hyphae of ΔCgscd1 were observed in the penetration experiments, suggesting that the penetration ability of nonpigmented appressoria was partially impaired. In conclusion, we identified the CgSCD1 gene, which is involved in melanin synthesis and pathogenicity, and found that the melanization defect did not affect appressorial turgor pressure in C. gloeosporioides.

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  • The ΔCgscd1 mutant shows complete absence of melanin accumulation during appressorial formation or on vegetative hyphae and exhibits reduced hyphal penetration ability.

    “The ΔCgscd1 mutant shows complete absence of melanin accumulation during appressorial formation or on vegetative hyphae and exhibits reduced hyphal penetration ability [57].”

    From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [57]

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Cited by

Sorted by citations

Authors
Wang, T., Ren, D., Guo, H., Chen, X., Zhu, P., Nie, H., & Xu, L.
Affiliations
East China Normal University (CN)
Corresponding author
  • Nie, Haozhen
  • Xu, Ling
ORCID
Venue
Pathogens
Publisher
MDPI AG
Published
2020-02-20
Volume
9
Issue
2
Pages
141
ISSN
2076-0817 (ISSN-L)
DOI
10.3390/pathogens9020141
PMC
PMC7169410
OpenAlex
W3008524576
Cited by
41
References
1
Citations by year
2026: 3 · 2025: 9 · 2024: 4 · 2023: 10 · 2022: 8 · 2021: 5 · 2020: 2
FWCI
3.09
Type
journal article
Language
en
Open access
Licence
Locations
  • Pathogens (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31571902; 31972121)
  • Shanghai Municipal Science and Technology Commission (18391901400)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
Keywords
Colletotrichum gloeosporioides, scytalone dehydratase, melanogenesis, fungal pathogenicity, anthracnose, fungal virulence, deletion mutants, hyphal penetration

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