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Cited by 555

The DOI matches its Crossref record; 108 of the 108 references it cites were found in a registry or an index.

Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

Open access
Rocchitta, Gaia, Spanu, Angela, Babudieri, Sergio, Latte, Gavinella, Madeddu, Giordano, Galleri, Grazia, Nuvoli, Susanna, Bagella, Paola, Demartis, Maria, Fiore, Vito, Manetti, Roberto, Serra, Pier·2016·Sensors

Abstract

Enzyme-based chemical biosensors are based on biological recognition. In order to operate, the enzymes must be available to catalyze a specific biochemical reaction and be stable under the normal operating conditions of the biosensor. Design of biosensors is based on knowledge about the target analyte, as well as the complexity of the matrix in which the analyte has to be quantified. This article reviews the problems resulting from the interaction of enzyme-based amperometric biosensors with complex biological matrices containing the target analyte(s). One of the most challenging disadvantages of amperometric enzyme-based biosensor detection is signal reduction from fouling agents and interference from chemicals present in the sample matrix. This article, therefore, investigates the principles of functioning of enzymatic biosensors, their analytical performance over time and the strategies used to optimize their performance. Moreover, the composition of biological fluids as a function of their interaction with biosensing will be presented.

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Crossref · 10.3390/s16060780

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Metadata

Authors
Rocchitta, G., Spanu, A., Babudieri, S., Latte, G., Madeddu, G., Galleri, G., Nuvoli, S., Bagella, P., Demartis, M., Fiore, V., Manetti, R., & Serra, P.
Venue
Sensors
Publisher
MDPI AG
Published
2016-05-30
Volume
16
Issue
6
Pages
780
ISSN
1424-8220 (ISSN-L)
OpenAlex
W2419031153
Cited by
555
References
108
FWCI
19.06
Type
journal article
Language
en
Open access
gold

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CoChat. (2026). Citation check: Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids. https://app.cochat.ai/papers/10.3390/s16060780
The paper
Rocchitta, G., Spanu, A., Babudieri, S., Latte, G., Madeddu, G., Galleri, G., Nuvoli, S., Bagella, P., Demartis, M., Fiore, V., Manetti, R., & Serra, P. (2016). Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids. Sensors. https://doi.org/10.3390/s16060780

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.