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Cited by 31Wound Healing and TreatmentsPhytochemistry and biological activity of medicinal plantsVeterinary Medicine and Surgery

The Protective Effect of Snail Secretion Filtrate in an Experimental Model of Excisional Wounds in Mice

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Gugliandolo, Enrico, Macrì, Francesco, Fusco, Roberta, Siracusa, Rosalba, D’Amico, Ramona, Cordaro, Marika, Peritore, Alessio Filippo, Impellizzeri, Daniela +5 more·2021·Veterinary Sciences
DOI 10.3390/vetsci8080167PMC PMC8402640
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Abstract

Wound healing is a physiological process comprising several coordinated phases, such as inflammation, proliferation, and remodeling. For centuries, Helix aspersa Muller mucus has been known to have biological properties that are useful for treating skin disorders. In this study, we used a full-thickness excisional wound model in mice to test the hypothesis that Snail Secretion Filtrate (SSF) can improve the wound healing process. The mucus from Helix aspersa Muller was obtained mechanically by manually stimulating snails with a sterile cotton swab tip, and then the mucus was subjected to a series of filtrations to obtain SSF. After wounding, the mice were treated topically with SSF for 14 days. Our macroscopic results show that the SSF treatment significantly improved the speed and percentage of wound area closure. Furthermore, SSF improved several markers of proper wound healing, such as collagen deposition (Masson, COL3A1, matrix metalloproteinases (MMPs)) and the tissue remodeling process (α-sma, vascular-endothelial growth factor (VEGF)). SSF was also able to counteract the inflammatory process in injured wound tissue (myeloperoxidase (MPO) IL-1β, IL-6, TNF-α). In conclusion, our results show that SSF is able to enhance the speed and efficiency of wound healing and positively regulate several aspects of the wound healing process, such as the proliferative and remodeling phases.

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Authors
Gugliandolo, E., Macrì, F., Fusco, R., Siracusa, R., D’Amico, R., Cordaro, M., Peritore, A. F., Impellizzeri, D., Genovese, T., Cuzzocrea, S., Paola, R. D., Licata, P., & Crupi, R.
Affiliations
  • University of Messina (IT)
  • Department of Veterinary Science, University of Messina, 98166 Messina, Italy
Corresponding author
  • Cuzzocrea, Salvatore
  • Paola, Rosanna Di
ORCID
Venue
Veterinary Sciences
Publisher
MDPI AG
Published
2021-08-20
Volume
8
Issue
8
Pages
167
ISSN
2306-7381 (ISSN-L)
DOI
10.3390/vetsci8080167
PMC
PMC8402640
OpenAlex
W3193649826
Cited by
31
References
3
Citations by year
2026: 6 · 2025: 7 · 2024: 6 · 2023: 9 · 2022: 3
FWCI
2.36
Type
journal article
Language
en
Open access
Licence
Locations
  • Veterinary Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activity of medicinal plants
  • Health Sciences › Veterinary › Small Animals › Veterinary Medicine and Surgery
Keywords
Helix aspersa, wound healing, excision wound, topical treatment, collagen deposition, matrix metalloproteinases, tissue remodeling, inflammation, vascular endothelial growth factor, myeloperoxidase, cytokines, full-thickness wounds

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