The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Serum‐free culture alters the quantity and protein composition of neuroblastoma‐derived extracellular vesicles
Open accessAbstract
Extracellular vesicles (EVs) play a significant role in cell–cell communication in numerous physiological processes and pathological conditions, and offer promise as novel biomarkers and therapeutic agents for genetic diseases. Many recent studies have described different molecular mechanisms that contribute to EV biogenesis and release from cells. However, little is known about how external stimuli such as cell culture conditions can affect the quantity and content of EVs. While N2a neuroblastoma cells cultured in serum‐free (OptiMEM) conditions did not result in EVs with significant biophysical or size differences compared with cells cultured in serum‐containing (pre‐spun) conditions, the quantity of isolated EVs was greatly increased. Moreover, the expression levels of certain vesicular proteins (e.g. small GTPases, G‐protein complexes, mRNA processing proteins and splicing factors), some of which were previously reported to be involved in EV biogenesis, were found to be differentially expressed in EVs under different culture conditions. These data, therefore, contribute to the understanding of how extracellular factors and intracellular molecular pathways affect the composition and release of EVs.
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Cites
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Cited by
Théry, C., Witwer, K. W., Aikawa, E., Alcaraz, M. J., Anderson, J. D., Andriantsitohaina, R., Antoniou, A., Arab, T., Archer, F., Atkin‐Smith, G. K., et al. (2018) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2018.1535750 · Cited by 11,099
Self-citationApplying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper
Lener, T., Gimona, M., Aigner, L., Börger, V., Buzas, E., Camussi, G., Chaput, N., Chatterjee, D., Court, F. A., del Portillo, H. A., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.30087 · Cited by 1,491
Self-citationGurunathan, S., Kang, M.-H., Jeyaraj, M., Qasim, M., & Kim, J.-H. (2019) · Cells · doi:10.3390/cells8040307 · Cited by 1,122
Advances in therapeutic applications of extracellular vesicles
Wiklander, O. P. B., Brennan, M. Á., Lötvall, J., Breakefield, X. O., & EL Andaloussi, S. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav8521 · Cited by 1,039
Self-citationMethodological Guidelines to Study Extracellular Vesicles
Coumans, F. A. W., Brisson, A. R., Buzas, E. I., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., Emanueli, C., Gasecka, A., Hendrix, A., Hill, A. F., et al. (2017) · Circulation Research · doi:10.1161/circresaha.117.309417 · Cited by 1,017
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Metadata
- Authors
- Li, J., Lee, Y., Johansson, H. J., Mäger, I., Vader, P., Nordin, J. Z., Wiklander, O. P. B., Lehtiö, J., Wood, M. J. A., & Andaloussi, S. E.
- Venue
- Journal of Extracellular Vesicles
- Publisher
- Wiley
- Published
- 2015-01
- Volume
- 4
- Issue
- 1
- Pages
- 26883-26883
- ISSN
- 2001-3078 (ISSN-L)
- OpenAlex
- W2158347593
- Cited by
- 184
- References
- 7
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- gold
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- Li, J., Lee, Y., Johansson, H. J., Mäger, I., Vader, P., Nordin, J. Z., Wiklander, O. P. B., Lehtiö, J., Wood, M. J. A., & Andaloussi, S. E. (2015). Serum‐free culture alters the quantity and protein composition of neuroblastoma‐derived extracellular vesicles. Journal of Extracellular Vesicles. https://doi.org/10.3402/jev.v4.26883
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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