Cited by 365Extracellular vesicles in diseaseNeuroinflammation and Neurodegeneration MechanismsCellular transport and secretion

Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism

Potolicchio, Ilaria, Carven, Gregory J, Xu, Xiaonan, Stipp, Christopher, Riese, Richiard J, Stern, Lawrence J, Santambrogio, Laura·2005·The Journal of Immunology

Abstract

Vesicle transport is a fundamental mechanism of communication in the CNS. In this study we characterized a novel type of vesicle released by murine brain microglial cells: microglial exosomes. Analysis of their protein content identified several enzymes, chaperones, tetraspanins, and membrane receptors previously reported in B cells and dendritic cell-derived exosomes. Additionally, microglia-derived exosomes expressed the aminopeptidase CD13 and the lactate transporter MCT-1. Exosomal CD13 was metabolically active in cleaving leucine- and methionine-enkephalins peptides by releasing the N-terminal tyrosine. Cleaved neuropeptides were unable to bind to the neuronal opioid receptor as assessed by cAMP response. Microglial exosomal vesicles may represent an important, previously unrecognized, cellular communication system in an organ in which cell motility is highly restricted.

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Crossref · 10.4049/jimmunol.175.4.2237

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Authors
Potolicchio, I., Carven, G. J., Xu, X., Stipp, C., Riese, R. J., Stern, L. J., & Santambrogio, L.
Affiliations
  • Albert Einstein College of Medicine (US)
  • University of Massachusetts Chan Medical School (US)
  • Dana-Farber Cancer Institute (US)
  • Brigham and Women's Hospital (US)
Venue
The Journal of Immunology
Published
2005-08
Volume
175
Issue
4
Pages
2237-2243
ISSN
  • 0022-1767 (ISSN-L)
  • 1550-6606
OpenAlex
W1524589668
Cited by
365
References
37
Citations by year
2026: 11 · 2025: 6 · 2024: 18 · 2023: 22 · 2022: 32 · 2021: 31 · 2020: 30 · 2019: 29 · 2018: 16 · 2017: 18 · 2016: 29 · 2015: 13 · 2014: 16 · 2013: 14 · 2012: 22
FWCI
3.39
Type
journal article
Language
en
Open access
closed
Locations
  • The Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
Funders
  • National Institutes of Health (5k08ai001555-03)
  • National Cancer Institute (CA 86712, R01CA086712)
  • National Institute of Allergy and Infectious Diseases (K08AI01555, R01-AI38996, R01AI048832, R01AI038996)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Keywords
CD13, aminopeptidase, enkephalin, opioid receptors, MCT1, lactate transporters, tetraspanins, cell surface receptors, molecular chaperones
MeSH terms
Animals, Antigens, Differentiation, B-Lymphocyte, Histocompatibility Antigens Class II, Cathepsins, Cell Communication, Cell Line, Neuropeptides, Endosomes, Biomarkers, Microglia, CD13 Antigens, Proteome, Cytoplasmic Vesicles, Cell Line, Tumor, Mice

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