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Multipotent Menstrual Blood Stromal Stem Cells: Isolation, Characterization, and Differentiation
Open accessAbstract
The stromal stem cell fraction of many tissues and organs has demonstrated to exhibit stem cell properties such as the capability of self-renewal and multipotency, allowing for multilineage differentiation. In this study, we characterize a population of stromal stem cells derived from menstrual blood (MenSCs). We demonstrate that MenSCs are easily expandable to clinical relevance and express multipotent markers such as Oct-4, SSEA-4, and c-kit at the molecular and cellular level. Moreover, we demonstrate the multipotency of MenSCs by directionally differentiating MenSCs into chondrogenic, adipogenic, osteogenic, neurogenic, and cardiogenic cell lineages. These studies demonstrate the plasticity of MenSCs for potential research in regenerative medicine.
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Cites
Derivation of human embryonic stem cells in defined conditions
Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1177 · Cited by 1,114
Clonogenicity of Human Endometrial Epithelial and Stromal Cells1
Chan, R. W. S., Schwab, K. E., & Gargett, C. E. (2004) · Biology of Reproduction · doi:10.1095/biolreprod.103.024109 · Cited by 653
Cited by
Endometrial stem/progenitor cells: the first 10 years
Gargett, C. E., Schwab, K. E., & Deane, J. A. (2015) · Human Reproduction Update · doi:10.1093/humupd/dmv051 · Cited by 561
Adult stem cells in the endometrium
Gargett, C. E., & Masuda, H. (2010) · Molecular Human Reproduction · doi:10.1093/molehr/gaq061 · Cited by 361
A novel marker of human endometrial mesenchymal stem-like cells
Masuda, H., Anwar, S. S., Bühring, H.-J., Rao, J. R., & Gargett, C. E. (2012) · Cell Transplantation · doi:10.3727/096368911x637362 · Cited by 294
Stem cells in endometrium and their role in the pathogenesis of endometriosis
Figueira, P. G. M., Abrão, M. S., Krikun, G., & Taylor, H. (2011) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2011.05969.x · Cited by 188
Endometrial stem/progenitor cells and their role in the pathogenesis of endometriosis
Cousins, F. L., O, D. F., & Gargett, C. E. (2018) · Best Practice & Research Clinical Obstetrics & Gynaecology · doi:10.1016/j.bpobgyn.2018.01.011 · Cited by 158
Metadata
- Authors
- Patel, A. N., Park, E., Kuzman, M., Benetti, F., Silva, F. J., & Allickson, J. G.
- Venue
- Cell Transplantation
- Publisher
- SAGE Publications
- Published
- 2008-03
- Volume
- 17
- Issue
- 3
- Pages
- 303-311
- ISSN
- 0963-6897 (ISSN-L)
- 1555-3892
- OpenAlex
- W2013074450
- Cited by
- 366
- References
- 2
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- green
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- Patel, A. N., Park, E., Kuzman, M., Benetti, F., Silva, F. J., & Allickson, J. G. (2008). Multipotent Menstrual Blood Stromal Stem Cells: Isolation, Characterization, and Differentiation. Cell Transplantation. https://doi.org/10.3727/096368908784153922
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