Land plant evolutionary timeline: Gene effects are secondary to fossil constraints in relaxed clock estimation of age and substitution rates

Abstract

• Premise of the study: Land plants play an essential role in the evolution of terrestrial life. Their time of origin and diversification is fundamental to understanding the evolution of life on land. We investigated the timing and the rate of molecular evolution of land plants, evaluating the effects of different types of molecular data, including temporal information from fossils, and using different molecular clock methods. • Methods: Ages and absolute rates were estimated independently with two substitutionally different data sets: a highly conserved 4‐gene data set and matK, a fast‐evolving gene. The vascular plant backbone and the crown nodes of all major lineages were calibrated with fossil‐derived ages. Dates and absolute rates were estimated while including or excluding the calibrations and using two relaxed clocks that differ in their implementation of temporal autocorrelation. • Key results: Land plants diverged from streptophyte alga 912 (870–962) million years ago (Mya) but diversified into living lineages 475 (471–480) Mya. Ages estimated for all major land‐plant lineages agree with their fossil record, except for angiosperms. Different genes estimated very similar ages and correlated absolute rates across the tree. Excluding calibrations resulted in the greatest age differences. Different relaxed clocks provided similar ages, but different and uncorrelated absolute rates. • Conclusions: Whole‐genome rate accelerations or decelerations may underlie the similar ages and correlated absolute rates estimated with different genes. We suggest that pronounced substitution rate changes around the angiosperm crown node may represent a challenge for relaxed clocks to model adequately.