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Cited by 23Liver physiology and pathologyOrgan Transplantation Techniques and OutcomesTissue Engineering and Regenerative Medicine

Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration

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Tsomaia, Keti, Patarashvili, Leila, Karumidze, Nino, Bebiashvili, Irakli, Az-maipharashvili, Elza, Modebadze, Irina, Dzidziguri, Diana, Sareli, Marom +2 more·2020·World Journal of Gastroenterology
DOI 10.3748/wjg.v26.i27.3899PMC PMC7385567
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Abstract

BACKGROUND: The phenomenon of liver regeneration after partial hepatectomy (PH) is still a subject of considerable interest due to the increasing frequency of half liver transplantation on the one hand, and on the other hand, new surgical approaches which allow removal of massive space-occupying hepatic tumors, which earlier was considered as inoperable. Interestingly, the mechanisms of liver regeneration are extensively studied after PH but less attention is paid to the architectonics of the regenerated organ. Because of this, the question "How does the structure of regenerated liver differ from normal, regular liver?" has not been fully answered yet. Furthermore, almost without any attention is left the liver's structural transformation after repeated hepatectomy (of the re-regenereted liver). AIM: To compare the architectonics of the lobules and circulatory bed of normal, re-generated and re-regenerated livers. METHODS: study group (SG2); 16 rats were subjected to sham operation - control group (CG); The livers were studied after 9 months from PH, and after 6 months from repeated PH. Cytological (Schiff reaction for the determination of DNA concen-tration), histological (H&E, Masson trichrome, CK8 Immunohistochemical marker, transparent slides after Indian Ink injection, ), morphometrical (hepatocytes areas, perimeters and ploidy) and Electron Microscopical (Scanning Electron Microscopy of corrosion casts) methods were used. RESULTS: is featured by the slightly expressed ductular reaction. CONCLUSION: Regenerated and re-regenerated livers in comparison with normal liver contain hypertrophied hepatocytes with increased ploidy which together with transformed sinusoidal and biliary meshworks form the remodeled lobulli.

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  • Boundaries between adjacent lobules, including mega-lobules, are widened and filled by connective tissue fibers, giving the liver parenchyma a nodular look.

    “The boundaries between the adjacent lobules (including mega-lobules) are widened and filled by connective tissue fibers, which gives the liver parenchyma a nodular look (Figure 3C)[72].”

    From Liver regeneration after partial hepatectomy: Triggers and mechanisms (2025) · cites this paper as [72]

  • Our study showed different regeneration dynamics depending on the zones of the liver acinus.

    “These data are consistent with the results of our study, which showed different regeneration dynamics depending on the zones of the liver acinus[72,73].”

    From Liver regeneration after partial hepatectomy: Triggers and mechanisms (2025) · cites this paper as [72]

  • After repeat resection of a previously regenerated liver, regeneration is re-initiated using the same mechanisms and continues until liver mass is once again restored.

    “Furthermore, if this balance is disrupted again - for example, following a repeat resection of a previously regenerated liver - regeneration is re-initiated using the same mechanisms and continues until liver mass is once again restored, as we have shown in our experimental model[72].”

    From Liver regeneration after partial hepatectomy: Triggers and mechanisms (2025) · cites this paper as [72]

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Authors
Tsomaia, K., Patarashvili, L., Karumidze, N., Bebiashvili, I., Az-maipharashvili, E., Modebadze, I., Dzidziguri, D., Sareli, M., Gusev, S., & Kordzaia, D.
Affiliations
  • Tbilisi State University (GE)
  • Sheba Medical Center (IL)
ORCID
Venue
World Journal of Gastroenterology
Publisher
Baishideng Publishing Group Inc.
Published
2020-07-21
Volume
26
Issue
27
Pages
3899-3916
ISSN
1007-9327 (ISSN-L)
DOI
10.3748/wjg.v26.i27.3899
PMC
PMC7385567
OpenAlex
W3043860344
Cited by
23
References
3
Citations by year
2026: 3 · 2025: 3 · 2024: 2 · 2023: 8 · 2022: 3 · 2021: 4
FWCI
2.21
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • World Journal of Gastroenterology (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
liver regeneration, partial hepatectomy, hepatocyte hypertrophy, hepatocyte ploidy, ductular reaction
MeSH terms
Animals, Hepatectomy, Liver, Liver Regeneration, Male, Rats, Wistar, Hepatocytes, Rats

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