Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells
Open accessAbstract
Structural interactions between the endoplasmic reticular (ER) and mitochondrial membranes, in domains known as mitochondria-associated membranes (MAM), are crucial hubs for cellular signaling and cell fate. Particularly, these inter-organelle contact sites allow the transfer of calcium from the ER to mitochondria through the voltage-dependent anion channel (VDAC)/glucose-regulated protein 75 (GRP75)/inositol 1,4,5-triphosphate receptor (IP3R) calcium channeling complex. While this subcellular compartment is under intense investigation in both physiological and pathological conditions, no simple and sensitive method exists to quantify the endogenous amount of ER-mitochondria contact in cells. Similarly, MAMs are highly dynamic structures, and there is no suitable approach to follow modifications of ER-mitochondria interactions without protein overexpression. Here, we report an optimized protocol based on the use of an in situ proximity ligation assay to visualize and quantify endogenous ER-mitochondria interactions in fixed cells by using the close proximity between proteins of the outer mitochondrial membrane (VDAC1) and of the ER membrane (IP3R1) at the MAM interface. Similar in situ proximity ligation experiments can also be performed with the GRP75/IP3R1 and cyclophilin D/IP3R1 pairs of antibodies. This assay provides several advantages over other imaging procedures, as it is highly specific, sensitive, and suitable to multiple-condition testing. Therefore, the use of this in situ proximity ligation assay should be helpful to better understand the physiological regulations of ER-mitochondria interactions, as well as their role in pathological contexts.
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VDAC1 and IP3R1 proximity were measured using an in situ proximity ligation assay that was thoroughly validated previously.
“VDAC1 (ab14734) and IP3R1 (sc28614) proximity were measured using an in situ proximity ligation assay [PLA; Sigma-Aldrich Duolink anti-rabbit plus (DUO92002) and anti-mouse minus (DUO92004) kits] to detect and quantify SR-mitochondria interactions, as described and thoroughly validated previously (Tubbs and Rieusset, 2016; Tubbs et al., 2014).”
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- Authors
- Tubbs, E., & Rieusset, J.
- Affiliations
- Lund University (SE)
- Lyon 1 Université (FR)
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2016-12-10
- Issue
- 118
- Pages
- np
- ISSN
- 1940-087X (ISSN-L)
- PMC
- PMC5226377
- OpenAlex
- W2560338825
- Cited by
- 60
- References
- 20
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 10 · 2023: 4 · 2022: 7 · 2021: 5 · 2020: 6 · 2019: 9 · 2018: 9 · 2017: 3
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Visualized Experiments (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-11-BSV1-0033, ANR-09-JCJC-0116)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- endoplasmic reticulum-mitochondria interaction, mitochondria-associated membranes, in situ proximity ligation assay, IP3R1, VDAC1, GRP75, cyclophilin D, calcium signaling, fixed cells, subcellular protein localization
- MeSH terms
- Animals, Biological Assay, Endoplasmic Reticulum, Humans, Intracellular Membranes, Mitochondria, Signal Transduction, Mitochondrial Membranes, Inositol 1,4,5-Trisphosphate Receptors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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