Potentially probiotic Lactobacillus strains with anti-proliferative activity induce cytokine/chemokine production and neutrophil recruitment in mice
Abstract
Lactobacillus pentosus B281 and Lactobacillus plantarum B282 are two Lactobacillus strains previously isolated from fermented table olives. Both strains were found to possess probiotic properties and displayed desirable technological characteristics for application as starters in novel functional food production. In the present study the anti-proliferative and immunostimulatory activities of the two strains were investigated. Firstly, we demonstrated that live L. pentosus B281 and L. plantarum B282 significantly inhibited the growth of human colon cancer cells (Caco-2) in a time- and dose-dependent manner. By employing the air pouch system in mice, we showed that administration of both strains led to a rapid and statistically significant infiltration of leukocytes in the air pouch exudates. The phenotypical characterisation of the recruited immune cells was performed by flow cytometry analysis. We demonstrated that the majority of the infiltrated leukocytes were neutrophils. Finally by using the Mouse Cytokine Array Panel A Detection Antibody cocktail, we showed that both strains induced the expression of granulocyte-colony stimulating factor, interleukin (IL)-1α, IL-1β, IL-6, chemokine (C-X-C motif) ligand (CXCL)-1, chemokine (C-C motif) ligand (CCL)-3, CCL-4, and CXCL-2 and diminished the expression levels of soluble intercellular adhesion molecule, macrophage colony-stimulating factor and metallopeptidase inhibitor 1. Our results showed that both strains display anti-proliferative and immunostimulatory properties equal or even better in some cases than those of established and commonly used probiotic strains. These findings further support the probiotic character of the two strains.
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The most studied activities of probiotic LAB from fermented olives are antibacterial, antifungal, anti-adherent, antiproliferative, anticancer, metal detox, and immunostimulant.
“The most studied activities were mainly antibacterial, antifungal, anti-adherent, antiproliferative, anticancer, metal detox, and immunostimulant [7-9].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [9]
Many recent works have demonstrated several health benefits of probiotic LAB found in fermented olives.
“Many recent works have demonstrated several health benefits of probiotic LAB found in fermented olives.”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [9]
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- Authors
- Saxami, G., Karapetsas, A., Chondrou, P., Vasiliadis, S., Lamprianidou, E., Kotsianidis, I., Ypsilantis, P., Botaitis, S., Simopoulos, C., & Galanis, A.
- Affiliations
- Democritus University of Thrace (GR)
- Department of Hematology, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.
- Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
- Venue
- Beneficial Microbes
- Publisher
- Walter de Gruyter GmbH
- Published
- 2017-08-24
- Volume
- 8
- Issue
- 4
- Pages
- 615-624
- ISSN
- 1876-2883 (ISSN-L)
- 1876-2891
- OpenAlex
- W2625799217
- Cited by
- 31
- References
- 31
- Citations by year
- 2025: 5 · 2024: 2 · 2023: 3 · 2022: 6 · 2021: 9 · 2020: 2 · 2019: 3 · 2017: 1
- FWCI
- 1.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Beneficial Microbes (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- Lactobacillus pentosus, Lactobacillus plantarum, probiotic bacteria, antiproliferative activity, immune enhancement, colon cancer cells, Caco-2 cells, air pouch model, neutrophil recruitment, cytokine production, chemokines, granulocyte colony-stimulating factor, interleukins, functional foods
- MeSH terms
- Animals, Female, Humans, Immunologic Factors, Inflammation, Lactobacillus, Male, Mice, Inbred BALB C, Neutrophils, Cytokines, Chemokines, Caco-2 Cells, Probiotics, Neutrophil Infiltration, Olea, Cell Proliferation, Mice
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