Using of DNA-Barcoding, SCoT and SDS-PAGE Protein to Assess Soma-Clonal Variation in Micro-Propagated Fig (Ficus carica L.) Plant
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Background and Objective: In vitro propagation of fig (Ficus carica L.) is one of the possible approaches that may be used to maximize the diversity of plant species. The current work was carried out to evaluate genetic stability of micropropagated fig plantlets and to determine the effect of in vitro propagation on genomic content of Saudi fig. Materials and Methods: The start codon-targeted (SCoT), DNA-barcoding chloroplast gene RNA polymerase1 (rpoC1 sequencing) and total protein profiling assays (SDS-PAGE) techniques were used to detect genetic stability in micropropagated fig plantlets. Results: The Scorable PCR bands were produced with 10 SCoT primers used, where the total number of bands was 135 bands. Twenty polymorphic bands were generated with 18.4% of a polymorphism percentage. According to the result, no visual unique bands were generated which confirmed the genetic homogeneity of micropropagated plantlets samples compared to the control sample (mother plant). Sequence analysis and phylogenetic tree generated using fig rpoC1 sequence showed high similarity between control and plantlets samples of fig plant. The protein profiling results revealed no remarkable changes between micropropagated plantlets and the mother plant. Conclusion: The results indicate that using SCoT, DNA barcoding and protein profiling have demonstrated their utility to detect genetic homogeneity in micropropagated fig plantlets, which suggests using of micropropagation protocol of plants applied on the plantlets in the current study as a reliable protocol for in vitro culture and conservation of fig plant.
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The most used DNA markers are ISSR, RAPD, RFLP, SSR, AFLP, and SCoT.
“The most used DNA markers are inter simple sequence repeats (ISSR), random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), microsatellites (SSR), amplified fragment length polymorphism (AFLP), and start codon-targeted (SCoT) polymorphism [47,48,49,50,51].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [51]
The Introduction (page not provided) names ISSR, RAPD, AFLP and SCoT among the most commonly used assays for assessing somaclonal variation. It does not include RFLP or SSR in that list, and its statement is specific to somaclonal-variation assessment rather than DNA markers generally. (LLM, conf=0.88)
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- Authors
- O. Attia, A., A. Ismail, I., S. Dessoky, E. D., & S. Aljuaid, B.
- ORCID
- O. Attia, Attia
- Venue
- Pakistan Journal of Biological Sciences
- Publisher
- Science Alert
- Published
- 2022-05-15
- Volume
- 25
- Issue
- 5
- Pages
- 415-425
- ISSN
- 1028-8880 (ISSN-L)
- OpenAlex
- W4225131033
- Cited by
- 6
- References
- 24
- Citations by year
- 2025: 1 · 2023: 5
- FWCI
- 0.49
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- other-oa
- Locations
- Pakistan Journal of Biological Sciences (publishedVersion, other-oa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activities of Ficus species
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- Ficus carica, figs, somaclonal variation, micropropagation, genetic stability, rpoC1, chloroplast DNA barcoding, SCoT markers, proteomic profiling, plant conservation
- MeSH terms
- Electrophoresis, Polyacrylamide Gel, Genetic Markers, Phylogeny, Codon, Initiator, DNA, Plant, Ficus
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