Shifts in Rumen Fermentation and Microbiota Are Associated with Dissolved Ruminal Hydrogen Concentrations in Lactating Dairy Cows Fed Different Types of Carbohydrates
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Background Different carbohydrates ingested greatly influence rumen fermentation and microbiota and gaseous methane emissions. Dissolved hydrogen concentration is related to rumen fermentation and methane production. Objectives We tested the hypothesis that carbohydrates ingested greatly alter the rumen environment in dairy cows, and that dissolved hydrogen concentration is associated with these changes in rumen fermentation and microbiota. Methods Twenty-eight lactating Chinese Holstein dairy cows [aged 4-5 y, body weight 480 ± 37 kg (mean ± SD)] were used in a randomized complete block design to investigate effects of 4 diets differing in forage content (45% compared with 35%) and source (rice straw compared with a mixture of rice straw and corn silage) on feed intake, rumen fermentation, and microbial populations. Results Feed intake (10.7-12.6 kg/d) and fiber degradation (0.584-0.692) greatly differed (P ≤ 0.05) between cows fed the 4 diets, leading to large differences (P ≤ 0.05) in gaseous methane yield (27.2-37.3 g/kg organic matter digested), dissolved hydrogen (0.258-1.64 μmol/L), rumen fermentation products, and microbiota. Ruminal dissolved hydrogen was negatively correlated (r 0.40; P Conclusions Changes in fermentation pathways from acetate toward propionate production and in microbiota from fibrolytic toward amylolytic species were closely associated with ruminal dissolved hydrogen in lactating dairy cows. An unresolved paradox was that greater dissolved hydroge…
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Elevated fermentative acetate production is consistently associated with an increase in methane production.
“Generally, elevated fermentative acetate production is consistently associated with an increase in methane production [52, 53].”
From Reductive acetogenesis is a dominant process in the ruminant hindgut (2025) · cites this paper as [52]
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Self-citation
- Authors
- Wang, M., Wang, R., Xie, T. Y., Janssen, P. H., Sun, X. Z., Beauchemin, K. A., Tan, Z. L., & Gao, M.
- Affiliations
- Chinese Academy of Sciences (CN)
- AgResearch (NZ)
- Agriculture and Agri-Food Canada (CA)
- Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences (CN)
- Corresponding author
- Tan, Zhi Liang
- Venue
- The Journal of Nutrition
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 146
- Issue
- 9
- Pages
- 1714-1721
- ISSN
- 0022-3166 (ISSN-L)
- OpenAlex
- W2503358543
- Cited by
- 91
- References
- 36
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 8 · 2023: 15 · 2022: 16 · 2021: 7 · 2020: 7 · 2019: 6 · 2018: 5 · 2017: 8
- FWCI
- 13.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- rumen fermentation, rumen microbiota, lactating dairy cows, carbohydrate sources, rice straw, corn silage, methane emissions, methanogenic archaea, Fibrobacter succinogenes, Selenomonas ruminantium, Prevotella, acetate, propionate, butyrate, Gibbs free energy, fiber degradation, feed intake
- MeSH terms
- Gastrointestinal Microbiome, Animals, Animal Feed, Cattle, Diet, Dietary Carbohydrates, Female, Fermentation, Hydrogen, Lactation, Methane, Models, Theoretical, Rumen, Prevotella, Selenomonas, Fibrobacter
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