Kinetic Studies of Alkaline Protease from Bacillus licheniformis NCIM-2042
Abstract
An extensive investigation was carried out to describe the kinetics of cell growth, substrate consumption, and product formation in the batch fermentation using starch as substrate. Evaluation of intrinsic kinetic parameters was carried out using a best-fit unstructured model. A nonlinear regression technique was applied for computational purpose. The Andrew's model showed a comparatively better R2 value among all tested models. The values of specific growth rate (micronmax), saturation constant (KS), inhibition constant (KI), and YX/S were found to be 0.109 h-1, 11.1 g/l, 0.012 g/l, and 1.003, respectively. The Leudeking- Piret model was used to study the product formation kinetics and the process was found to be growth-associated. The growth-associated constant (alpha) for protease production was sensitive to substrate concentration. Its value was fairly constant up to a substrate concentration of 30.8 g/l, and then decreased.
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The lower R2 value of 0.8713 in Monod's model suggested reasonable substrate or product inhibition on growth of the organism.
“The lower correlation coefficient (R2) value of 0.8713 found in Monod’s model suggested that there was reasonable substrate or product inhibition on growth of the organism (Bhunia et al. 2012).”
From Kinetics of rapamycin production by Streptomyces hygroscopicus MTCC 4003 (2014) · cites this paper as Bhunia et al. 2012
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- Authors
- Bhunia, B.
- Affiliations
- Heritage Institute of Technology (IN)
- Corresponding author
- Bhunia, Biswanath
- ORCID
- Bhunia, Biswanath
- Venue
- Journal of Microbiology and Biotechnology
- Published
- 2012-12-28
- Volume
- 22
- Issue
- 12
- Pages
- 1758-1766
- ISSN
- 1017-7825 (ISSN-L)
- 1738-8872
- OpenAlex
- W2323996003
- Cited by
- 29
- References
- 0
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 7 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 6 · 2015: 2 · 2014: 1 · 2013: 2
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- alkaline protease, Bacillus licheniformis, batch fermentation, enzyme production kinetics, starch substrate, cell growth kinetics, substrate consumption, product formation kinetics, Leudeking-Piret model, specific growth rate, yield coefficient, inhibition constant
- MeSH terms
- Bacillus, Bacterial Proteins, Computer Simulation, Culture Media, Fermentation, Kinetics, Models, Biological, Endopeptidases, Starch, Nonlinear Dynamics, Biomass, Bioreactors
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