Aptamer Based SPREETA Sensor for the Detection of Porphyromonas gingivalis G-Protein
Open accessAbstract
We have developed an aptamer that specifically binds to Porphyromonas gingivalis to reduce the cellular damage caused by P. gingivalis infection and applied it as a biosensor. P. gingivalis is one of the major pathogens causing destructive periodontal disease among the periodontal microorganisms constituting complex biofilms. Porphyromonas gingivalis G-protein (PGP) known to play an important role in the transmission of germs was used as a target protein for the screening of aptamer. The aptamer that has binds to the G-protein of P. gingivalis, was screened and developed through the Systemic Evolution of Ligands by Exponential Energy (SELEX) method. Modified-Western blot analysis was performed with the aptamer which consisted of 38 single-stranded DNA to confirm the selectivity. ELONA (enzyme linked oligonucleotide assay) used to confirm that the aptamer was sensitive to PGP even at low concentration of 1 μg/ml. For the rapid detection of P. gingivalis, we constructed a surface plasmon resonance biosensor with SPREETA using the PGP aptamer. It was confirmed that PGP could be detected as low concentration as at 0.1 pM, which is the minimum concentration of aptamer sensor within 5 min. Based on these results, we have constructed a SPREETA biosensor based on aptamer that can bind to P. gingivalis G-protein. It can be used as an infection diagnosis system to rapidly diagnose and analyze oral diseases caused by P. gingivalis.
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Compared with conventional qPCR assays, the overall detection time of the PDA-based sensing platform is reduced to approximately 20 min.
“Compared with conventional qPCR assays, the overall detection time is reduced to approximately 20 min, highlighting its potential for rapid periodontal pathogen screening.75,90”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [90]
The Discussion reports rapid detection of P. gingivalis G-protein with an aptamer-coated SPREETA sensor, with binding completed within 600 seconds. It does not describe a PDA-based platform, establish an overall detection time of approximately 20 minutes, or compare detection time with conventional qPCR. No page numbers are provided in our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measuredThe 16S rRNA of Porphyromonas gingivalis contains GC-rich hairpin regions, which provide suitable targets for nucleic acid-based sensing.
“The 16S rRNA of Porphyromonas gingivalis contains GC-rich hairpin regions, which provide suitable targets for nucleic acid–based sensing.”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [90]
The Introduction and Results describe a nucleic-acid aptamer selected to bind P. gingivalis G-protein, not 16S rRNA. Searches for 16S rRNA, GC-rich regions, and hairpins found no passage in our copy addressing the claimed RNA structure or its suitability as a sensing target. (LLM, conf=0.77)
Experimental — accuracy not yet measuredWhen evaluated in an artificial saliva matrix, the PDA-based sensing platform for P. gingivalis exhibits a dynamic detection range of 10^2-10^7 CFU mL−1, a limit of detection of 38 CFU mL−1, and recovery rates of 93% to 105%.
“When evaluated in an artificial saliva matrix, this sensing platform exhibits a dynamic detection range of 102–107 CFU mL−1, a limit of detection (LOD) of 38 CFU mL−1, and recovery rates ranging from 93% to 105%.”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [90]
The Abstract and Results describe an aptamer-coated SPREETA sensor detecting P. gingivalis G-protein, with concentrations reported in pM or μg/ml. Our copy does not address a PDA platform evaluated in artificial saliva or report the claimed CFU detection range, 38 CFU/ml limit, or 93%–105% recoveries (searched for those terms and values). (LLM, conf=0.76)
Experimental — accuracy not yet measured
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- Authors
- Park, S.-G., Lee, H. J., Ji, T., Kim, K., & Ohk, S.-H.
- Affiliations
- Chonnam National University (KR)
- Kwangju Women's University (KR)
- Corresponding author
- Ohk, Seung-Ho
- Venue
- Journal of Microbiology and Biotechnology
- Published
- 2023-12-18
- Volume
- 34
- Issue
- 2
- Pages
- 289-295
- ISSN
- 1017-7825 (ISSN-L)
- 1738-8872
- PMC
- PMC10940744
- OpenAlex
- W4389958798
- Cited by
- 2
- References
- 29
- Citations by year
- 2025: 1 · 2024: 1
- FWCI
- 0.18
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by
- Locations
- Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Chonnam National University Hospital Biomedical Research Institute (CRI 15006-1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- aptamers, Porphyromonas gingivalis, bacterial detection, biosensors
- MeSH terms
- Humans, Oligonucleotides, Periodontal Diseases, Biosensing Techniques, Porphyromonas gingivalis, GTP-Binding Proteins
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