The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
MicroRNA-21 Is Up-Regulated in Allergic Airway Inflammation and Regulates IL-12p35 Expression
Open accessAbstract
Allergic airway inflammation is characterized by marked in situ changes in gene and protein expression, yet the role of microRNAs (miRNAs), a new family of key mRNA regulatory molecules, in this process has not yet been reported. Using a highly sensitive microarray-based approach, we identified 21 miRNAs with differential expression between doxycycline-induced lung-specific IL-13 transgenic mice (with allergic airway inflammation) and control mice. In particular, we observed overexpression of miR-21 and underexpression of miR-1 in the induced IL-13 transgenic mice compared with control mice. These findings were validated in two independent models of allergen-induced allergic airway inflammation and in IL-4 lung transgenic mice. Although IL-13-induced miR-21 expression was IL-13Rα1 dependent, allergen-induced miR-21 expression was mediated mainly independent of IL-13Rα1 and STAT6. Notably, predictive algorithms identified potential direct miR-21 targets among IL-13-regulated lung transcripts, such as IL-12p35 mRNA, which was decreased in IL-13 transgenic mice. Introduction of pre-miR-21 dose dependently inhibited cellular expression of a reporter vector harboring the 3′-untranslated region of IL-12p35. Moreover, mutating miR-21 binding sites in IL-12p35 3′-untranslated region abrogated miR-21-mediated repression. In summary, we have identified a miRNA signature in allergic airway inflammation, which includes miR-21 that modulates IL-12, a molecule germane to Th cell polarization.
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
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Combinatorial microRNA target predictions
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A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
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The impact of microRNAs on protein output
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Widespread changes in protein synthesis induced by microRNAs
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“Although some of the targets of miRNAs are modulated through translational inhibition only, the majority of targets have mRNA level changes inverse to their respective miRNA regulator (49, 50).”
BackgroundAsangani, I. A., Rasheed, S. A. K., Nikolova, D. A., Leupold, J. H., Colburn, N. H., Post, S., & Allgayer, H. (2008) · Oncogene · doi:10.1038/sj.onc.1210856 · Cited by 1,882
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MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators
Gabriely, G., Wurdinger, T., Kesari, S., Esau, C. C., Burchard, J., Linsley, P. S., & Krichevsky, A. M. (2008) · Molecular and Cellular Biology · doi:10.1128/mcb.00479-08 · Cited by 888 · cited 1×
“Notably, MiR-21 has been shown to regulate the level of the matrix metalloprotease inhibitor RECK (42, 73, 74), and indeed we have identified this gene to be down-regulated by IL-13 in the lung (Table II); it is interesting to speculate that this could account for some of increased matrix metalloprotease activity seen…”
BackgroundMicroRNA-21 promotes cell transformation by targeting the programmed cell death 4 gene
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miR-21 plays a pivotal role in gastric cancer pathogenesis and progression
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Cited by
Physiological and pathological roles for microRNAs in the immune system
O'Connell, R. M., Rao, D. S., Chaudhuri, A. A., & Baltimore, D. (2010) · Nature Reviews Immunology · doi:10.1038/nri2708 · Cited by 1,378
Sheedy, F. J., Palsson-McDermott, E., Hennessy, E. J., Martin, C., O'Leary, J. J., Ruan, Q., Johnson, D. S., Chen, Y., & O'Neill, L. A. J. (2010) · Nature Immunology · doi:10.1038/ni.1828 · Cited by 1,005
Inflammation and cancer: interweaving microRNA, free radical, cytokine and p53 pathways
Schetter, A. J., Heegaard, N. H. H., & Harris, C. C. (2010) · Carcinogenesis · doi:10.1093/carcin/bgp272 · Cited by 657
Regulation and function of miRNA-21 in health and disease
Kumarswamy, R., Volkmann, I., & Thum, T. (2011) · RNA Biology · doi:10.4161/rna.8.5.16154 · Cited by 607
Pan, W., Zhu, S., Yuan, M., Cui, H., Wang, L., Luo, X., Li, J., Zhou, H., Tang, Y., & Shen, N. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0904060 · Cited by 561
Metadata
- Authors
- Lu, T. X., Munitz, A., & Rothenberg, M. E.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-04
- Volume
- 182
- Issue
- 8
- Pages
- 4994-5002
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1526700155
- Cited by
- 570
- References
- 12
- FWCI
- 24.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lu, T. X., Munitz, A., & Rothenberg, M. E. (2009). MicroRNA-21 Is Up-Regulated in Allergic Airway Inflammation and Regulates IL-12p35 Expression. The Journal of Immunology. https://doi.org/10.4049/jimmunol.0803560
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