The DOI matches its Crossref record.
Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity.
Abstract
A novel monocyte-derived neutrophil-activating peptide (MONAP) produced by lipopolysaccharide- and phorbol myristate acetate-stimulated human peripheral blood monocytes was purified by sequential ion exchange-high performance liquid chromatography (HPLC), size exclusion HPLC, and reversed phase HPLC. Biologic activities of the purified cytokine were monitored by either an enzyme release assay or a chemotaxis assay, using peripheral human neutrophils. Purified MONAP was found to be homogeneous, giving a single peak on size-exclusion HPLC, reversed-phase HPLC, as well as a single 10-kDa band on silver-stained polyacrylamide gels. Purified MONAP stimulate human neutrophil chemotaxis at an estimated molarity of 5 x 10(-11) M. Half-maximal enzyme release of cytochalasin B pretreated neutrophils occurred at 2 to 3 x 10(-10) M, whereas superoxide anion production elicited by various concentrations of MONAP was found to be low. Isolated human peripheral monocytes, as well as human eosinophils, showed no chemotactic response to MONAP, indicating neutrophil specificity. MONAP activity was separated from thymocyte-stimulating activity by reversed-phase HPLC, indicating nonidentity with interleukin (IL)-1. This was further supported by heat resistance of MONAP, which is in contrast to the heat sensitivity of IL-1. In addition, IL-1 obtained as a by-product during isolation of MONAP did not stimulate human neutrophil chemotaxis.
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Citations
Cited by
Macrophages and Polymorphonuclear Neutrophils in Lung Defense and Injury
Sibille, Y., & Reynolds, H. Y. (1990) · American Review of Respiratory Disease · doi:10.1164/ajrccm/141.2.471 · Cited by 1,083
Essential involvement of interleukin-8 (IL-8) in acute inflammation
Harada, A., Sekido, N., Akahoshi, T., Wada, T., Mukaida, N., & Matsushima, K. (1994) · Journal of Leukocyte Biology · doi:10.1002/jlb.56.5.559 · Cited by 1,041
Rollins, B., Walz, A., & Baggiolini, M. (1991) · Blood · doi:10.1182/blood.v78.4.1112.1112 · Cited by 369
Walz, A., Burgener, R., Car, B., Baggiolini, M., Kunkel, S. L., & Strieter, R. M. (1991) · The Journal of experimental medicine · doi:10.1084/jem.174.6.1355 · Cited by 353
Kimura, A., Kinjyo, I., Matsumura, Y., Mori, H., Mashima, R., Harada, M., Chien, K. R., Yasukawa, H., & Yoshimura, A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.c300496200 · Cited by 119
Metadata
- Authors
- Schröder, J. M., Mrowietz, U., Morita, E., & Christophers, E.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1987-11
- Volume
- 139
- Issue
- 10
- Pages
- 3474-3483
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1524903400
- Cited by
- 531
- References
- 0
- FWCI
- 14.45
- Type
- journal article
- Language
- en
- Open access
- closed
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- Schröder, J. M., Mrowietz, U., Morita, E., & Christophers, E. (1987). Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity. The Journal of Immunology. https://doi.org/10.4049/jimmunol.139.10.3474
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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