The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Peripheral Blood Fibrocytes: Differentiation Pathway and Migration to Wound Sites
Abstract
Fibrocytes are a distinct population of blood-borne cells that display a unique cell surface phenotype (collagen I+/CD11b+/CD13+/CD34+/CD45RO+/MHC class II+/CD86+) and exhibit potent immunostimulatory activities. Circulating fibrocytes rapidly enter sites of tissue injury, suggesting an important role for these cells in wound repair. However, the regulatory processes that govern the differentiation of blood-borne fibrocytes and the mechanisms that underlie the migration of these cells to wound sites are currently not known. We report herein that ex vivo cultured fibrocytes can differentiate from a CD14+-enriched mononuclear cell population and that this process requires contact with T cells. Furthermore, we demonstrate that TGF-β1 (1–10 ng/ml), an important fibrogenic and growth-regulating cytokine involved in wound healing, increases the differentiation and functional activity of cultured fibrocytes. Because fibrocytes home to sites of tissue injury, we examined the role of chemokine/chemokine receptor interactions in fibrocyte trafficking. We show that secondary lymphoid chemokine, a ligand of the CCR7 chemokine receptor, acts as a potent stimulus for fibrocyte chemotaxis in vitro and for the homing of injected fibrocytes to sites of cutaneous tissue injury in vivo. Finally, we demonstrate that differentiated, cultured fibrocytes express α smooth muscle actin and contract collagen gels in vitro, two characteristic features of wound-healing myofibroblasts. These data provide important insight into the control of fibrocyte differentiation and trafficking during tissue repair and significantly expand their potential role during wound healing.
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“Their migration to wounds is guided by damage associated molecular patterns (DAMPs) and chemokines such as Chemokine (C-C motif) ligand 21 (CCL21) (86), and after arrival, these cells start differentiating into a myofibroblast-like phenotype under the influence of factors such as TGFβ (86).”
From Unraveling SSc Pathophysiology; The Myofibroblast (2018) · cites this paper as [86]
In the citing paper
“Their migration to wounds is guided by damage associated molecular patterns (DAMPs) and chemokines such as Chemokine (C-C motif) ligand 21 (CCL21) (86), and after arrival, these cells start differentiating into a myofibroblast-like phenotype under the influence of factors such as TGFβ (86).”
From Unraveling SSc Pathophysiology; The Myofibroblast (2018) · cites this paper as [86]
Citations
Cites
A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)
Bleul, C. C., Fuhlbrigge, R. C., Casasnovas, J. M., Aiuti, A., & Springer, T. A. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.3.1101 · Cited by 1,482
Circulating Fibrocytes Define a New Leukocyte Subpopulation That Mediates Tissue Repair
Bucala, R., Spiegel, L. A., Chesney, J. A., Hogan, M. M., & Cerami, A. (1994) · Molecular Medicine · doi:10.1007/bf03403533 · Cited by 1,307
Self-citationCD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells.
Zhou, L., & Tedder, T. F. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.6.2588 · Cited by 1,090
Sime, P. J., Xing, Z., Graham, F. L., Csaky, K. G., & Gauldie, J. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119590 · Cited by 1,050
Gunn, M. D., Kyuwa, S., Tam‐Amersdorfer, C., Kakiuchi, T., Matsuzawa, A., Williams, L. T., & Nakano, H. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.3.451 · Cited by 1,019
Myofibroblasts. I. Paracrine cells important in health and disease
Powell, D. W., Mifflin, R. C., Valentich, J. D., Crowe, S. E., Saada, J. I., & West, A. B. (1999) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1999.277.1.c1 · Cited by 974
Saeki, H., Moore, A. M., Brown, M. J., & Hwang, S. T. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.5.2472 · Cited by 623
Mechanisms of Myofibroblast Activity and Phenotypic Modulation
Serini, G., & Gabbiani, G. (1999) · Experimental Cell Research · doi:10.1006/excr.1999.4543 · Cited by 592
Branton, M., & Kopp, J. (1999) · Microbes and Infection · doi:10.1016/s1286-4579(99)00250-6 · Cited by 574
Arora, P. D., Narani, N., & McCulloch, C. A. (1999) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65334-5 · Cited by 474
Vaughan, M. B., Howard, E. W., & Tomasek, J. J. (2000) · Experimental Cell Research · doi:10.1006/excr.2000.4869 · Cited by 468
Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis–like lesions
Vestergaard, C., Yoneyama, H., Murai, M., Nakamura, K., Tamaki, K., Terashima, Y., Imai, T., Yoshie, O., Irimura, T., Mizutani, H., et al. (1999) · Journal of Clinical Investigation · doi:10.1172/jci7613 · Cited by 406
Pickl, W. F., Majdic, O., Kohl, P., Stöckl, J., Riedl, E., Scheinecker, C., Bello-Fernández, C., & Knapp, W. (1996) · The Journal of Immunology · doi:10.4049/jimmunol.157.9.3850 · Cited by 399
Regulated Production of Type I Collagen and Inflammatory Cytokines by Peripheral Blood Fibrocytes
Chesney, J. A., Metz, C. N., Stavitsky, A. B., Bacher, M., & Bucala, R. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.1.419 · Cited by 371
Self-citationInhibition of Myofibroblast Apoptosis by Transforming Growth Factor β1
Zhang, H. Y., & Phan, S. H. (1999) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.21.6.3720 · Cited by 354
Chesney, J. A., Bacher, M., Bender, A., & Bucala, R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.12.6307 · Cited by 343
Self-citationDifferentiation of human dendritic cells from monocytes in vitro
Chapuis, F., Rosenzwajg, M., Yagello, M., Ekman, M., Biberfeld, P., & Gluckman, J. C. (1997) · European Journal of Immunology · doi:10.1002/eji.1830270213 · Cited by 338
McCormick, L. L., Zhang, Y., Tootell, E., & Gilliam, A. C. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.10.5693 · Cited by 264
Matsuda, H., Koyama, H., Sato, H., Sawada, J., Itakura, A., Tanaka, A., Matsumoto, M., Konno, K., Ushio, H., & Matsuda, K. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.187.3.297 · Cited by 242
Kaliński, P., Schuitemaker, J. H. N., Hilkens, C. M. U., Wierenga, E. A., & Kapsenberg, M. L. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.6.3231 · Cited by 211
Lymphoid Tissue Homing Chemokines Are Expressed in Chronic Inflammation
Hjelmström, P., Fjell, J., Nakagawa, T., Sacca, R. E., Cuff, C. A., & Ruddle, N. H. (2000) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)64981-4 · Cited by 195
Nakano, H., Mori, S., Yonekawa, H., Nariuchi, H., Matsuzawa, A., & Kakiuchi, T. (1998) · Blood · doi:10.1182/blood.v91.8.2886.2886_2886_2895 · Cited by 186
The Role of α1β1 Integrin in Wound Contraction
Racine-Samson, L., Rockey, D. C., & Bissell, D. M. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.49.30911 · Cited by 176
Transforming growth factor β levels in rat wound chambers
Cromack, D. T., Sporn, M. B., Roberts, A. B., Merino, M. J., Dart, L. L., & Norton, J. A. (1987) · Journal of Surgical Research · doi:10.1016/0022-4804(87)90005-9 · Cited by 159
Pivotal role of TARC, a CC chemokine, in bacteria-induced fulminant hepatic failure in mice.
Yoneyama, H., Harada, A., Imai, T., Baba, M., Yoshie, O., Zhang, Y., Higashi, H., Murai, M., Asakura, H., & Matsushima, K. (1998) · Journal of Clinical Investigation · doi:10.1172/jci4619 · Cited by 141
Genetic defect in T lymphocyte‐specific homing into peripheral lymph nodes
Nakano, H., Tamura, T., Yoshimoto, T., Yagita∥, H., Miyasaka, M., Butcher, E. C., Nariuchi, H., Kakiuchi, T., & Matsuzawa, A. (1997) · European Journal of Immunology · doi:10.1002/eji.1830270132 · Cited by 137
Benn, S. I., Whitsitt, J. S., Broadley, K. N., Nanney, L. B., Perkins, D., He, L., Patel, M., Morgan, J. R., Swain, W. F., & Davidson, J. M. (1996) · Journal of Clinical Investigation · doi:10.1172/jci119118 · Cited by 108
Dendritic Cells Require T Cells for Functional Maturation In Vivo
Shreedhar, V. K., Moodycliffe, A. M., Ullrich, S. E., Bucana, C. D., Kripke, M. L., & Flores‐Romo, L. (1999) · Immunity · doi:10.1016/s1074-7613(00)80137-5 · Cited by 105
Gauldie, J., Sime, P. J., Xing, Z., Marr, B., & Tremblay, G. M. (1999) · Current topics in pathology · doi:10.1007/978-3-642-58456-5_5 · Cited by 93
Varona, R., Zaballos, Á., Gutiérrez, J., Martı́n, P., Roncal, F., Albar, J. P., Ardavı́n, C., & Márquez, G. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)01450-1 · Cited by 89
Wang, X., Smith, P. D., Pu, L. L. Q., Kim, Y., Ko, F., & Robson, M. C. (1999) · Journal of Surgical Research · doi:10.1006/jsre.1999.5757 · Cited by 76
Arora, P. D., & McCulloch, C. A. (1999) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65527-7 · Cited by 75
Fibroblast heterogeneity in physiological conditions and fibrotic disease
Jelaska, A., Strehlow, D., & Korn, J. H. (1999) · Springer Seminars in Immunopathology · doi:10.1007/bf00870301 · Cited by 74
Chesney, J. A., & Bucala, R. (1997) · Biochemical Society Transactions · doi:10.1042/bst0250520 · Cited by 67
Self-citationThe Fibroblast — A Ubiquitous Ally for the Surgeon
Dunphy, J. E. (1963) · New England Journal of Medicine · doi:10.1056/nejm196306202682501 · Cited by 62
Chu, A. J., & Prasad, J. K. (1999) · Journal of the American College of Surgeons · doi:10.1016/s1072-7515(98)00303-2 · Cited by 45
Grab, D. J., Lanners, H. N., Martin, L. N., Chesney, J. A., Cai, C., Adkisson, H. D., & Bucala, R. (1999) · Molecular Medicine · doi:10.1007/bf03402138 · Cited by 45
Self-citationXu, H., Kramer, M. M., Spengler, H., & Peters, J. H. (1995) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-1971-3_15 · Cited by 43
Chemokine Amplification in Mesangial Cells
Luo, Y., Lloyd, C. M., Gutierrez-Ramos, J.-C., & Dorf, M. E. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.7.3985 · Cited by 39
Adachi, S., Ito, H., Tamamori, M., Tanaka, M., Marumo, F., & Hiroe, M. (1998) · Life Sciences · doi:10.1016/s0024-3205(98)00452-4 · Cited by 30
Flagged first, then by citations
Research works printed but not linked: 42
- 1963, N. Engl. J. Med., 1367
- 1994, Mol. Med., 71
- 1997, Proc. Natl. Acad. Sci. USA, 6307
- 1999, Mol. Med., 46
- 1998, J. Immunol., 419
- 1999, Am. J. Pathol., 2087
- 1999, Am. J. Pathol., 871
- 1998, J. Exp. Med., 297
- 1996, J. Exp. Med., 1101
- 1998, Life Sci., 1779
- 1999, J. Clin. Invest., 1097
- 1998, J. Clin. Invest., 1933
- 1998, FEBS Lett., 188
- 1999, J. Immunol., 2472
- 1999, J. Immunol., 3985
- 1997, J. Biol. Chem., 30911
- 2000, Exp. Cell Res., 180
- 1999, Exp. Cell Res., 273
- 1999, Am. J. Physiol, C1
- 1998, J. Immunol., 419
- 1997, Biochem. Soc. Trans., 520
- 1995, Adv. Exp. Med. Biol., 75
- 1996, J. Immunol., 3850
- 1996, Proc. Natl. Acad. Sci. USA, 2588
- 1997, Eur. J. Immunol., 431
- 1999, Immunity, 625
- 1999, J. Immunol., 3231
- 1999, Microbes. Infect., 1349
- 1999, J. Am. Coll. Surg., 271
- 1999, Am. J. Respir. Cell Mol. Biol., 658
- 1999, J. Surg. Res., 194
- 1987, J. Surg. Res., 622
- 1996, J. Clin. Invest., 2894
- 1999, Springer Semin. Immunopathol., 385
- 1999, J. Immunol., 5693
- 1997, J. Clin. Invest., 768
- 1999, Curr. Top. Pathol., 35
- 1999, J. Immunol., 2472
- 2000, Am. J. Pathol., 1133
- 1999, J. Exp. Med., 451
- 1998, Blood, 2886
- 1997, Eur. J. Immunol., 215
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Hinz, B., Phan, S. H., Thannickal, V. J., Galli, A., Bochaton-Piallat, M.-L., & Gabbiani, G. (2007) · The American Journal of Pathology · doi:10.2353/ajpath.2007.070112 · Cited by 2,068
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Transition from inflammation to proliferation: a critical step during wound healing
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“If the wound condition is long lasting, fibroblasts in the wounds may also originate from fibrocytes, which is a group of bone marrow-derived mesenchymal progenitor cells [37, 38].”
Background
Metadata
- Authors
- Abe, R., Donnelly, S. C., Peng, T., Bucala, R., & Metz, C. N.
- Affiliations
- Laboratory of Vascular Biology, The Picower Institute for Medical Research , Manhasset, NY 11030
- Laboratory of Medical Biochemistry, The Picower Institute for Medical Research , Manhasset, NY 11030
- Corresponding author
- Abe, Riichiro
- Peng, Tina
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-06
- Volume
- 166
- Issue
- 12
- Pages
- 7556-7562
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W4248565951
- Cited by
- 967
- References
- 40
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 22 · 2023: 25 · 2022: 28 · 2021: 23 · 2020: 37 · 2019: 30 · 2018: 34 · 2017: 31 · 2016: 43 · 2015: 47 · 2014: 58 · 2013: 59 · 2012: 68
- FWCI
- 27.32
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Immunology and Microbiology › Immunology › Macrophage Migration Inhibitory Factor
- Keywords
- fibrocytes, fibrocyte differentiation, wound healing, TGF-β1, CCR7, tissue damage, myofibroblasts, alpha-smooth muscle actin, collagen gel contraction
- MeSH terms
- Animals, Blood Cells, Cell Differentiation, Cell Movement, Cells, Cultured, Collagen, Female, Fibroblasts, Fibrosis, Gels, Humans, Injections, Intravenous, Mice, Inbred BALB C, Stem Cells, Wound Healing, Transforming Growth Factor beta, Lipopolysaccharide Receptors, Receptors, Chemokine, Stem Cell Transplantation, Mice, Transforming Growth Factor beta1
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