The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
The Serotoninergic Receptors of Human Dendritic Cells: Identification and Coupling to Cytokine Release
Open accessAbstract
The neurotransmitter 5-hydroxytryptamine (5-HT), commonly known as serotonin, is stored at peripheral sites in mast cells and released from this peripheral source upon IgE cross-linking. In this study, we investigated the expression of serotoninergic receptors (5-HTR), the signaling pathway, and biological activity of 5-HT on human dendritic cells (DC), showing that immature and mature DC expressed mRNA for different serotoninergic receptors. Thereby, the mRNA of 5-HTR1B, 5-HTR1E, 5-HTR2A, 5-HTR2B, one splicing variant of the 5-HTR3, 5-HTR4, and 5-HTR7 receptors were detected. Immature DC preferentially expressed mRNA for the heptahelical 5-HTR1B, 5-HTR1E, and 5-HTR2B receptors, while mature DC mostly expressed 5-HTR4 and 5-HTR7. The mRNA expression level of the ligand-gated cation channel 5-HTR3 and the heptahelical 5-HTR2A did not significantly change during maturation. Isotype-selective receptor agonists allowed us to show that 5-HT stimulated 5-HTR3-dependent Ca2+ influx in immature and mature DC. Moreover, we revealed that 5-HTR1 and 5-HTR2 receptor stimulation induced intracellular Ca2+ mobilization via Gi/o proteins in immature, but not mature, DC. Activation of 5-HTR4 and 5-HTR7 induced cAMP elevation in mature DC. Functional studies indicated that activation of 5-HTR4 and 5-HTR7 enhanced the release of the cytokines IL-1β and IL-8, while reducing the secretion of IL-12 and TNF-α in mature DC. In summary, our study shows that 5-HT stimulated, in a maturation-dependent manner, different signaling pathways in DC. These data point to a role for 5-HT in regulating the immune response at peripheral sites.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
Dendritic cells and the control of immunity
Banchereau, J., & Steinman, R. M. (1998) · Nature · doi:10.1038/32588 · Cited by 14,336
International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin).
Hoyer, D., Clarke, D. E., Fozard, J. R., Hartig, P. R., Martin, G. R., Mylecharane, E. J., Saxena, P. R., & Humphrey, P. P. (1994) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06783-3 · Cited by 3,003
Dynamics of T Lymphocyte Responses: Intermediates, Effectors, and Memory Cells
Lanzavecchia, A., & Sallusto, F. (2000) · Science · doi:10.1126/science.290.5489.92 · Cited by 796
Lovenberg, T. W., Baron, B. M., de Lecea, L., Miller, J. D., Prosser, R. A., Rea, M. A., Foye, P. E., Racke, M., Slone, A. L., Siegel, B. W., et al. (1993) · Neuron · doi:10.1016/0896-6273(93)90149-l · Cited by 692
Ruat, M., Traiffort, E., Leurs, R., Tardivel-Lacombe, J., Diaz, J., Arrang, J. M., & Schwartz, J. C. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.18.8547 · Cited by 617
Cloning of a novel human serotonin receptor (5-HT7) positively linked to adenylate cyclase.
Bard, J. A., Zgombick, J., Adham, N., Vaysse, P., Branchek, T. A., & Weinshank, R. L. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49479-9 · Cited by 533
Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype.
Shen, Y., Monsma, F. J., Metcalf, M. A., Jose, P. A., Hamblin, M. W., & Sibley, D. R. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)46830-x · Cited by 507
Standardized determination of real-time PCR efficiency from a single reaction set-up
Tichopad, A. (2003) · Nucleic Acids Research · doi:10.1093/nar/gng122 · Cited by 455
Cloning, Characterization, and Chromosomal Localization of a Human 5‐HT6 Serotonin Receptor
Kohen, R., Metcalf, M. A., Khan, N., Druck, T., Huebner, K., Lachowicz, J. E., Meltzer, H. Y., Sibley, D. R., Roth, B. L., & Hamblin, M. W. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.66010047.x · Cited by 377
Cited by
Hancock, D. B., Eijgelsheim, M., Wilk, J. B., Gharib, S. A., Loehr, L. R., Marciante, K. D., Franceschini, N., van Durme, Y. M. T. A., Chen, T.-H., Barr, R. G., et al. (2010) · Nature Genetics · doi:10.1038/ng.500 · Cited by 632
Petra, A. I., Panagiotidou, S., Hatziagelaki, E., Stewart, J. M., Conti, P., & Theoharides, T. C. (2015) · Clinical Therapeutics · doi:10.1016/j.clinthera.2015.04.002 · Cited by 592
Rosser, E. C., Piper, C. J. M., Matei, D. E., Blair, P. A., Rendeiro, A. F., Orford, M., Alber, D. G., Krausgruber, T., Catalan, D., Klein, N., et al. (2020) · Cell Metabolism · doi:10.1016/j.cmet.2020.03.003 · Cited by 579
The effects of serotonin in immune cells
Herr, N., Bode, C., & Duerschmied, D. (2017) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2017.00048 · Cited by 497
Tryptophan metabolism and gut-brain homeostasis
Roth, W., Zadeh, K., Vekariya, R., Ge, Y., & Mohamadzadeh, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22062973 · Cited by 519
Metadata
- Authors
- Idzko, M., Panther, E., Stratz, C., Müller, T., Bayer, H., Zissel, G., Dürk, T., Sorichter, S., Di Virgilio, F., Geissler, M., Fiebich, B., Herouy, Y., Elsner, P., Norgauer, J., & Ferrari, D.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-05
- Volume
- 172
- Issue
- 10
- Pages
- 6011-6019
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1783335369
- Cited by
- 272
- References
- 36
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: The Serotoninergic Receptors of Human Dendritic Cells: Identification and Coupling to Cytokine Release. https://app.cochat.ai/papers/10.4049/jimmunol.172.10.6011
- The paper
- Idzko, M., Panther, E., Stratz, C., Müller, T., Bayer, H., Zissel, G., Dürk, T., Sorichter, S., Di Virgilio, F., Geissler, M., Fiebich, B., Herouy, Y., Elsner, P., Norgauer, J., & Ferrari, D. (2004). The Serotoninergic Receptors of Human Dendritic Cells: Identification and Coupling to Cytokine Release. The Journal of Immunology. https://doi.org/10.4049/jimmunol.172.10.6011
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
