The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Standardized determination of real-time PCR efficiency from a single reaction set-up
Open accessAbstract
We propose a computing method for the estimation of real-time PCR amplification efficiency. It is based on a statistic delimitation of the beginning of exponentially behaving observations in real-time PCR kinetics. PCR ground fluorescence phase, non-exponential and plateau phase were excluded from the calculation process by separate mathematical algorithms. We validated the method on experimental data on multiple targets obtained on the LightCycler platform. The developed method yields results of higher accuracy than the currently used method of serial dilutions for amplification efficiency estimation. The single reaction set-up estimation is sensitive to differences in starting concentrations of the target sequence in samples. Furthermore, it resists the subjective influence of researchers, and the estimation can therefore be fully instrumentalized.
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Cites
A new mathematical model for relative quantification in real-time RT-PCR
Pfaffl, M. W. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.9.e45 · Cited by 34,610
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Housekeeping genes as internal standards: use and limits
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Cited by
Pfaffl, M. W., Tichopad, A., Prgomet, C., & Neuvians, T. P. (2004) · Biotechnology Letters · doi:10.1023/b:bile.0000019559.84305.47 · Cited by 5,065
Self-citationAmplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
Ruijter, J. M., Ramakers, C., Hoogaars, W. M. H., Karlen, Y., Bakker, O., van den Hoff, M. J. B., & Moorman, A. F. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp045 · Cited by 3,043
Quantification of mRNA using real-time RT-PCR
Nolan, T., Hands, R. E., & Bustin, S. A. (2006) · Nature Protocols · doi:10.1038/nprot.2006.236 · Cited by 2,247
Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
Matschke, J., Lütgehetmann, M., Hagel, C., Sperhake, J. P., Schröder, A. S., Edler, C., Mushumba, H., Fitzek, A., Allweiss, L., Dandri, M., et al. (2020) · The Lancet Neurology · doi:10.1016/s1474-4422(20)30308-2 · Cited by 1,300
Svec, D., Tichopad, A., Novosadova, V., Pfaffl, M. W., & Kubista, M. (2015) · Biomolecular Detection and Quantification · doi:10.1016/j.bdq.2015.01.005 · Cited by 601
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Metadata
- Authors
- Tichopad, A.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-10-15
- Volume
- 31
- Issue
- 20
- Pages
- 122e-122
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2099206288
- Cited by
- 455
- References
- 23
- FWCI
- 6.54
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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