The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Aluminum Hydroxide Adjuvants Activate Caspase-1 and Induce IL-1β and IL-18 Release
Abstract
Aluminum hydroxide (Alum) is the only adjuvant approved for routine use in humans, although the basis for its adjuvanticity remains poorly understood. In this study, we show that Alum activates caspase-1 and induce secretion of mature IL-1β and IL-18. Human PBMC or dendritic cells stimulated with pure TLR4 and TLR2 agonists released only traces of IL-1β or IL-18, despite the fact that the IL-1β mRNA was readily induced by both TLR agonists. In contrast, cells costimulated with TLR agonists plus Alum released large amount of IL-1β and IL-18. Alum-induced IL-1β and IL-18 production was not due to enhancement of TLR signaling but rather reflected caspase-1 activation and in mouse dendritic cells occurred in a MyD88-independent fashion. Secretion of other proinflammatory cytokines such as IL-8 was not affected by Alum treatments. However, TLR-induced production of IL-10 was increased and that of IFN-γ-inducible protein decreased by Alum cotreatment. Considering the immunostimulatory activities of these cytokines and the ability of IL-1β to act as adjuvant, our results suggest a mechanism for the adjuvanticity of Alum.
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Cites
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Cited by
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Vaccine Adjuvants: Putting Innate Immunity to Work
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Crucial role for the Nalp3 inflammasome in the immunostimulatory properties of aluminium adjuvants
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Metadata
- Authors
- Li, H., Nookala, S., & Re, F.
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-04
- Volume
- 178
- Issue
- 8
- Pages
- 5271-5276
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1541731964
- Cited by
- 332
- References
- 30
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- closed
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- Li, H., Nookala, S., & Re, F. (2007). Aluminum Hydroxide Adjuvants Activate Caspase-1 and Induce IL-1β and IL-18 Release. The Journal of Immunology. https://doi.org/10.4049/jimmunol.178.8.5271
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