The Exocytosis Regulator Synaptotagmin V Controls Phagocytosis in Macrophages
Open accessAbstract
Synaptotagmins (Syts) play a key role in the regulation of Ca2+-triggered exocytosis and membrane fusion events, two crucial events associated to the phagocytic process. In the present study, we investigated the role of Syt V, a regulator of focal exocytosis, in phagocytosis. In macrophages, Syt V is localized on recycling endosomes and on filopodia-like structures and is recruited to the nascent phagosomes independently of the phagocytic receptor engaged. Silencing of Syt V by RNA interference revealed a role for this protein for phagocytosis, particularly under conditions of high membrane demand. In contrast, silencing of Syt V had no effect on the recruitment of the lysosomal marker LAMP1 to phagosomes, indicating that phagosome maturation is not regulated by Syt V. Collectively, these results illustrate the importance of Syt V in the regulation of an important innate function of macrophages. Furthermore, our results are consistent with the concept that focal exocytosis of endocytic organelles is a key event in phagocytosis and suggest that Syt V regulates this process.
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Syts constitute a group of membrane proteins that regulate vesicle docking and fusion in processes such as exocytosis and phagocytosis.
“Syts constitute a group of membrane proteins that regulate vesicle docking and fusion in processes such as exocytosis (11, 23) and phagocytosis (11, 24, 25).”
From Macrophage Cytokines: Involvement in Immunity and Infectious Diseases (2014) · cites this paper as [25]
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Ca2+ and synaptotagmin VII–dependent delivery of lysosomal membrane to nascent phagosomes
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Fusion, fission, and secretion during phagocytosis
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Synaptotagmin II could confer Ca2+ sensitivity to phagocytosis in human neutrophils
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Macrophage Cytokines: Involvement in Immunity and Infectious Diseases
Arango Duque, G., & Descoteaux, A. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00491 · Cited by 2,708 · see citing works
“Syts constitute a group of membrane proteins that regulate vesicle docking and fusion in processes such as exocytosis (11, 23) and phagocytosis (11, 24, 25).”
BackgroundSelf-citationSynaptotagmin XI regulates phagocytosis and cytokine secretion in macrophages
Arango Duque, G., Fukuda, M., & Descoteaux, A. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1202500 · Cited by 52 · see citing works
Self-citationArango Duque, G., Fukuda, M., Turco, S. J., Stäger, S., & Descoteaux, A. (2014) · The Journal of Immunology · doi:10.4049/jimmunol.1303043 · Cited by 48 · see citing works
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- Authors
- Vinet, A. F., Fukuda, M., & Descoteaux, A.
- Affiliations
- Institut National de la Recherche Scientifique (CA)
- Tohoku University (JP)
- Corresponding author
- Descoteaux, Albert
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-10
- Volume
- 181
- Issue
- 8
- Pages
- 5289-5295
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1865326064
- Cited by
- 42
- References
- 8
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 3 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 4
- FWCI
- 1.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- EspaceINRS (National Institute for Scientific Research (Canada)) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- phagocytosis, macrophages, recycling endosomes, phagosome maturation, LAMP1, membrane fusion, calcium-triggered exocytosis, innate immunity
- MeSH terms
- Animals, Cell Line, Exocytosis, Membrane Fusion, Phagocytosis, Phagosomes, Pseudopodia, Macrophages, Peritoneal, RNA Interference, Synaptotagmins, Mice, Lysosomal Membrane Proteins, Lysosomal-Associated Membrane Protein 1
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