Expression of the Protein Kinase C Substrate Pleckstrin in Macrophages: Association with Phagosomal Membranes
Open accessAbstract
Despite evidence suggesting that protein kinase C (PKC) isoforms are important in phagocytosis by Fcγ receptors, the mechanisms by which the substrates of these kinases act are largely unknown. We have investigated the role of one PKC substrate, pleckstrin, in cells of the monocyte/macrophage lineage. Pleckstrin expression in mouse macrophages was induced severalfold in response to bacterial LPS and IFN-γ. In unstimulated cells, the protein was largely confined to the cytosol. Upon ingestion of IgG-opsonized zymosan particles (OPZ), however, pleckstrin accumulated on the phagosomal membrane. This association was transient, being maximal after 15 min and declining thereafter. Similar kinetics of association was also seen for both filamentous actin and the δ isoform of PKC. Ingestion of OPZ was found to induce phosphorylation of pleckstrin. To examine whether phosphorylation was required for phagosomal association, pleckstrin was expressed in CHO-IIA cells that stably express the FcγRIIA receptor and are competent for phagocytosis of OPZ. In these cells, both wild-type pleckstrin and mutants in which the phosphoacceptor sites had been mutated to either alanine (nonphosphorylatable) or glutamine (pseudophosphorylated) were found to accumulate on OPZ phagosomes. Thus, association of pleckstrin with phagosomes is independent of its phosphorylation. Our findings suggest that pleckstrin may serve as an intracellular adaptor/targeting protein in response to particulate stimuli. By targeting interacting ligands to the phagosomal compartment, pleckstrin may serve to regulate phagocytosis and/or early steps during maturation of the phagosome.
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Both PKCα and PKCδ copurify with isolated phagosomal membranes, and some inhibitors of PKC activity have been reported to interfere with phagocytosis.
“both PKCα and PKCδ copurify with isolated phagosomal membranes (Allen and Aderem 1995; Brumell et al. 1999) and some inhibitors of PKC activity have been reported to interfere with phagocytosis (Zheleznyak and Brown 1992; Karimi et al. 1999).”
From Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis (2000) · cites this paper as Brumell et al. 1999
The passage on phagosomal membranes reports that PKCδ accumulates on purified phagosomal membranes. It says conventional PKC isoforms have been found on phagosomes, but does not identify PKCα or establish its copurification. The inhibitor clause is attributed to other citations, not Brumell et al. (1999). (LLM, conf=0.87)
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C.
Toullec, D., Pianetti, P., Coste, H., Bellevergue, P., Grand-Perret, T., Ajakane, M., Baudet, V., Boissin, P., Boursier, E., & Loriolle, F. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)98476-0 · Cited by 2,446 · see citing works
Signaling Through Scaffold, Anchoring, and Adaptor Proteins
Pawson, T., & Scott, J. D. (1997) · Science · doi:10.1126/science.278.5346.2075 · Cited by 2,256 · see citing works
Clapham, D. E. (1995) · Cell · doi:10.1016/0092-8674(95)90408-5 · Cited by 1,960 · see citing works
Cloning and Characterization of Inducible Nitric Oxide Synthase from Mouse Macrophages
Xie, Q. W., Cho, H. J., Calaycay, J. R., Mumford, R. A., Swiderek, K. M., Lee, T. D., Ding, A. H., Troso, T., & Nathan, C. (1992) · Science · doi:10.1126/science.1373522 · Cited by 1,888 · see citing works
Protein Kinase C: Structure, Function, and Regulation
Newton, A. C. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.48.28495 · Cited by 1,662 · see citing works
Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin
Lassing, I., & Lindberg, U. (1985) · Nature · doi:10.1038/314472a0 · Cited by 823 · see citing works
Pleckstrin homology domains bind to phosphatidylinositol-4,5-bisphosphate
Harlan, J. E., Hajduk, P. J., Yoon, H. S., & Fesik, S. W. (1994) · Nature · doi:10.1038/371168a0 · Cited by 761 · see citing works
Desjardins, M., Huber, L., Parton, R., & Griffiths, G. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.5.677 · Cited by 673 · see citing works
Modulation of gelsolin function by phosphatidylinositol 4,5-bisphosphate
Janmey, P. A., & Stossel, T. P. (1987) · Nature · doi:10.1038/325362a0 · Cited by 622 · see citing works
Binding of G protein beta gamma-subunits to pleckstrin homology domains.
Touhara, K., Inglese, J., Pitcher, J. A., Shaw, G., & Lefkowitz, R. J. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34048-6 · Cited by 483 · see citing works
PH Domains: Diverse Sequences with a Common Fold Recruit Signaling Molecules to the Cell Surface
Lemmon, M. A., Ferguson, K. M., & Schlessinger, J. (1996) · Cell · doi:10.1016/s0092-8674(00)81022-3 · Cited by 467 · see citing works
A Comparative Analysis of the Phosphoinositide Binding Specificity of Pleckstrin Homology Domains
Rameh, L. E., Arvidsson, A., Carraway, K. L., Couvillon, A. D., Rathbun, G. A., Crompton, A. M., VanRenterghem, B., Czech, M., Ravichandran, K. S., Burakoff, S. J., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.35.22059 · Cited by 460 · see citing works
Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains
Kavran, J. M., Klein, D. E., Lee, A., Falasca, M., Isakoff, S. J., Skolnik, E. Y., & Lemmon, M. A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.46.30497 · Cited by 434 · see citing works
Requirement of phosphatidylinositol 4,5-bisphosphate for α-actinin function
Fukami, K., Furuhashi, K., Inagaki, M., Endo, T., Hatano, S., & Takenawa, T. (1992) · Nature · doi:10.1038/359150a0 · Cited by 328 · see citing works
The molecular dissection of Fc gamma receptor mediated phagocytosis
Indik, Z. K., Park, J., HUNTER, S. E., & Schreiber, A. (1995) · Blood · doi:10.1182/blood.v86.12.4389.bloodjournal86124389 · Cited by 313 · see citing works
Self-citationAllen, L. H., & Aderem, A. (1995) · The Journal of experimental medicine · doi:10.1084/jem.182.3.829 · Cited by 296 · see citing works
PLECKSTRIN HOMOLOGY DOMAINS: A Common Fold with Diverse Functions
Rebecchi, M. J., & Scarlata, S. (1998) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.27.1.503 · Cited by 291 · see citing works
Allen, L. H., & Aderem, A. A. (1996) · Current Opinion in Immunology · doi:10.1016/s0952-7915(96)80102-6 · Cited by 267 · see citing works
Molecular cloning and expression of the major protein kinase C substrate of platelets
Tyers, M. D., Rachubinski, R. A., Stewart, M. I., Varrichio, A., Shorr, R., Haslam, R. J., & Harley, C. B. (1988) · Nature · doi:10.1038/333470a0 · Cited by 255 · see citing works
Self-citationHaslam, R. J., & Lynham, J. A. (1977) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(77)80037-5 · Cited by 216 · see citing works
Signal transduction of phagocytosis
Greenberg, S. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)88957-6 · Cited by 183 · see citing works
Yonezawa, N., Homma, Y., Yahara, I., Sakai, H., & Nishida, E. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)47361-4 · Cited by 175 · see citing works
Pleckstrin's repeat performance: a novel domain in G-protein signaling?
Ponting, C. P., & Peer Bork, B. (1996) · PubMed · Cited by 152 · see citing works
Lukács, G. L., Rotstein, O. D., & Grinstein, S. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54263-0 · Cited by 139 · see citing works
Self-citationMembrane trafficking along the phagocytic pathway
Berón, W., Alvarez-Dominguez, C., Mayorga, L., & Stahl, P. D. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)88958-8 · Cited by 129 · see citing works
Brown, E. J. (1995) · BioEssays · doi:10.1002/bies.950170206 · Cited by 128 · see citing works
Pleckstrin's repeat performance: a novel domain in G-protein signaling?
Ponting, C. P., & Peer Bork, B. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)30027-3 · Cited by 120 · see citing works
Phospholipid-binding protein domains
Bottomley, M. J., Salim, K., & Panayotou, G. (1998) · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · doi:10.1016/s0005-2760(98)00141-6 · Cited by 118 · see citing works
Zheleznyak, A., & Brown, E. J. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49803-7 · Cited by 118 · see citing works
Opsonized zymosan stimulates the redistribution of protein kinase C isoforms in human neutrophils
Sergeant, S., & McPhail, L. C. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.159.6.2877 · Cited by 99 · see citing works
Interactions between Protein Kinase C and Pleckstrin Homology Domains
Yao, L., Suzuki, H., Ozawa, K., Deng, J., Lehel, C., Fukamachi, H., Anderson, W. B., Kawakami, Y., & Kawakami, T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.20.13033 · Cited by 97 · see citing works
Tyrosine phosphorylation and Fcγ receptor‐mediated phagocytosis
Strzelecka‐Kiliszek, A., Kwiatkowska, K., & Sobota, A. (1997) · FEBS Letters · doi:10.1016/s0014-5793(96)01359-2 · Cited by 81 · see citing works
Ma, A. D., Brass, L. F., & Abrams, C. S. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.136.5.1071 · Cited by 75 · see citing works
Toker, A., Bachelot‐Loza, C., Chen, C., Falck, J. R., Hartwig, J. H., Cantley, L. C., & Kovacsovics, T. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.49.29525 · Cited by 74 · see citing works
UPDATE: PH Domains--A Universal Membrane Adapter
Hemmings, B. A. (1997) · Science · doi:10.1126/science.275.5308.1899 · Cited by 68 · see citing works
Fc receptor-mediated signal transduction
Lin, C. T., Shen, Z., Boros, P., & Unkeless, J. C. (1994) · Journal of Clinical Immunology · doi:10.1007/bf01541170 · Cited by 60 · see citing works
Phagocytic processing of antigens for presentation by MHC molecules
Harding, C. V. (1995) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)88959-x · Cited by 60 · see citing works
Gresham, H. D., Mcgarr, J., Shackelford, P. G., & Brown, E. J. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113716 · Cited by 59 · see citing works
Zhang, J., Falck, J. R., Reddy, K. K., Abrams, C. S., Zhao, W., & Rittenhouse-Simmons, S. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.39.22807 · Cited by 55 · see citing works
Abrams, C. S., Zhao, W., Belmonte, E., & Brass, L. F. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.40.23317 · Cited by 53 · see citing works
Abrams, C. S., Wu, H., Zhao, W., Belmonte, E., White, D., & Brass, L. F. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.24.14485 · Cited by 46 · see citing works
Phosphopleckstrin Inhibits Gβγ-activable Platelet Phosphatidylinositol-4,5-bisphosphate 3-Kinase
Abrams, C. S., Zhang, J. C., Downes, C. P., Tang, X., Zhao, W., & Rittenhouse-Simmons, S. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.41.25192 · Cited by 42 · see citing works
Phosphorylation of Platelet Pleckstrin Activates Inositol Polyphosphate 5-Phosphatase I
Auethavekiat, V., Abrams, C. S., & Majerus, P. W. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.3.1786 · Cited by 40 · see citing works
Gailani, D., Fisher, T. C., Mills, D. C. B., & Macfarlane, D. E. (1990) · British Journal of Haematology · doi:10.1111/j.1365-2141.1990.tb02565.x · Cited by 39 · see citing works
Induction of the 47 kDa platelet substrate of protein kinase C during differentiation of HL-60 cells
Tyers, M., Rachubinski, R. A., Sartori, C., Harley, C. B., & Haslam, R. J. (1987) · Biochemical Journal · doi:10.1042/bj2430249 · Cited by 31 · see citing works
Self-citationPhosphorylation of human pleckstrin on Ser-113 and Ser-117 by protein kinase C
Craig, K. L., & Harley, C. B. (1996) · Biochemical Journal · doi:10.1042/bj3140937 · Cited by 29 · see citing works
Self-citationPhosphorylation and subcellular redistribution of pleckstrin in human neutrophils
Brumell, J. H., Craig, K. L., Ferguson, D. T., Tyers, M., & Grinstein, S. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.158.10.4862 · Cited by 29 · see citing works
Self-citationNanda, A., & Grinstein, S. (1995) · Journal of Cellular Physiology · doi:10.1002/jcp.1041650317 · Cited by 25 · see citing works
Self-citationSerine/threonine kinase activation in human neutrophils: relationship to tyrosine phosphorylation
Brumell, J. H., & Grinstein, S. (1994) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1994.267.6.c1574 · Cited by 25 · see citing works
Self-citationCalmodulin binding to platelet plasma membranes
Grinstein, S., & Furuya, W. (1982) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(82)90151-1 · Cited by 21 · see citing works
Self-citationActivity of mouse macrophages purified by adherence to, and removal from, a plastic surface
Wood, P. R., Simes, R. J., & NELSON, D. S. (1979) · Journal of Immunological Methods · doi:10.1016/0022-1759(79)90333-8 · Cited by 20 · see citing works
Ros�, S. D., Byers, D. M., Morash, S. C., FEDOROFF, S., & Cook, H. W. (1996) · Journal of Neuroscience Research · doi:10.1002/(sici)1097-4547(19960501)44:3 · Cited by 20 · see citing works
Modulation of actin polymerization by 47,000-dalton protein of human platelets.
Hashimoto, K., Im, T., Im, T., Tatsumi, N., Okuda, K., & Yukioka, M. (1987) · PubMed · Cited by 11 · see citing works
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Nrf2 is a critical regulator of the innate immune response and survival during experimental sepsis
Thimmulappa, R. K. (2006) · Journal of Clinical Investigation · doi:10.1172/jci25790 · Cited by 1,028 · see citing works
Phagocytosis and the actin cytoskeleton
May, R. C., & Machesky, L. M. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.6.1061 · Cited by 715 · see citing works
Localized Biphasic Changes in Phosphatidylinositol-4,5-Bisphosphate at Sites of Phagocytosis
Botelho, R. J., Teruel, M., Dierckman, R., Anderson, R., Wells, A., York, J. D., Meyer, T., & Grinstein, S. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.7.1353 · Cited by 539 · see citing works
“In agreement with this postulate, both PKCa and PKCd copurify with isolated phagosomal membranes (Allen and Aderem, 1995; Brumell et al., 1999) and some inhibitors of PKC activity have been reported to interfere with phagocytosis (Zheleznyak and Brown, 1992; Karimi et al.”
BackgroundSelf-citationFocal Exocytosis of Vamp3-Containing Vesicles at Sites of Phagosome Formation
Bajno, L., Peng, X., Schreiber, A. D., Moore, H.-P. H., Trimble, W. S., & Grinstein, S. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.149.3.697 · Cited by 319 · see citing works
Self-citationSignal transduction during Fc receptor-mediated phagocytosis
García-García, E., & Rosales, C. (2002) · Journal of Leukocyte Biology · doi:10.1189/jlb.72.6.1092 · Cited by 249 · see citing works
Identification of phagocytosis regulators using magnetic genome-wide CRISPR screens
Haney, M. S., Bohlen, C. J., Morgens, D. W., Ousey, J. A., Barkal, A. A., Tsui, C. K., Ego, B. K., Levin, R., Kamber, R. A., Collins, H., et al. (2018) · Nature Genetics · doi:10.1038/s41588-018-0254-1 · Cited by 193 · see citing works
Zheng, P., & Jones, N. L. (2003) · Cellular Microbiology · doi:10.1046/j.1462-5822.2003.00250.x · Cited by 190 · see citing works
Hmama, Z., Sendide, K., Talal, A., García, R., Dobos, K. M., & Reiner, N. E. (2004) · Journal of Cell Science · doi:10.1242/jcs.01072 · Cited by 151 · see citing works
Masur, K., Lang, K., Niggemann, B., Zänker, K. S., & Entschladen, F. (2001) · Molecular Biology of the Cell · doi:10.1091/mbc.12.7.1973 · Cited by 115 · see citing works
A role for PKC-ε in FcγR-mediated phagocytosis by RAW 264.7 cells
Larsen, E. C., Ueyama, T., Brannock, P. M., Shirai, Y., Saito, N., Larsson, C., Loegering, D. J., Weber, P. B., & Lennartz, M. R. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200205140 · Cited by 89 · see citing works
Sung, C., Nardelli, B., LaFleur, D. W., Blatter, E. E., Corcoran, M. L., Olsen, H. S., Birse, C. E., Pickeral, O. K., Zhang, J., Shah, D., et al. (2003) · Journal of Interferon & Cytokine Research · doi:10.1089/10799900360520423 · Cited by 71 · see citing works
Global Expression Analysis of Extracellular Matrix–Integrin Interactions in Monocytes
de Fougerolles, A. R., Rosso, G. C., Bajardi, A., Gotwals, P. J., Green, C. D., & Koteliansky, V. (2000) · Immunity · doi:10.1016/s1074-7613(00)00073-x · Cited by 71 · see citing works
Lundmark, A., Davanian, H., Båge, T., Johannsen, G., Koro, C., Lundeberg, J., & Yucel‐Lindberg, T. (2015) · Scientific Reports · doi:10.1038/srep18475 · Cited by 66 · see citing works
The leukocyte cytoskeleton in cell migration and immune interactions
Vicente‐Manzanares, M., Sancho, D., Yáñez‐Mó, M., & Sánchez‐Madrid, F. (2002) · International review of cytology · doi:10.1016/s0074-7696(02)16007-4 · Cited by 64 · see citing works
Ding, Y. J., Kantarcı, A., Badwey, J. A., Hastürk, H., Malabanan, A. O., & Dyke, T. E. V. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.1.647 · Cited by 63 · see citing works
Plaque contact and unimpaired Trem2 is required for the microglial response to amyloid pathology
Wood, J. I., Wong, E., Joghee, R., Balbaa, A., Vitanova, K. S., Stringer, K. M., VanSchoiack, A., Phelan, S.-L. J., Launchbury, F., Desai, S., et al. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111686 · Cited by 61 · see citing works
Kinget, L., Naulaerts, S., Govaerts, J., Vanmeerbeek, I., Sprooten, J., Laureano, R. S., Dubroja, N., Shankar, G., Bosisio, F. M., Roussel, E., et al. (2024) · Nature Medicine · doi:10.1038/s41591-024-02978-9 · Cited by 60 · see citing works
Altmaier, E., Kastenmüller, G., Römisch‐Margl, W., Thorand, B., Weinberger, K. M., Adamski, J., Illig, T., Döring, A., & Suhre, K. (2009) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.200900116 · Cited by 57 · see citing works
Loss of pleckstrin defines a novel pathway for PKC-mediated exocytosis
Lian, L., Wang, Y., Flick, M. J., Choi, J. K., Scott, E. W., Degen, J. L., Lemmon, M. A., & Abrams, C. S. (2009) · Blood · doi:10.1182/blood-2008-09-178913 · Cited by 57 · see citing works
Allen, L. H., & McCaffrey, R. L. (2007) · Immunological Reviews · doi:10.1111/j.1600-065x.2007.00544.x · Cited by 55 · see citing works
Phosphoinositide Involvement in Phagocytosis and Phagosome Maturation
Botelho, R. J., Scott, C. C., & Grinstein, S. (2004) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-18805-3_1 · Cited by 50 · see citing works
Self-citationRegulation of monocyte gene expression by the extracellular matrix and its functional implications
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Fetal brain response to maternal inflammation requires microglia
Lessi, M., Iturza, N. D., Stogsdill, J., Faits, T., Kim, K., Levin, J. Z., & Arlotta, P. (2024) · Development · doi:10.1242/dev.202252 · Cited by 46 · see citing works
The Exocytosis Regulator Synaptotagmin V Controls Phagocytosis in Macrophages
Vinet, A. F., Fukuda, M., & Descoteaux, A. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.8.5289 · Cited by 42 · see citing works
Tanimura, A., Nezu, A., Morita, T., Hashimoto, N., & Tojyo, Y. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m201130200 · Cited by 42 · see citing works
Atypical Protein Kinase C-ζ Is Essential for Delayed Phagocytosis of Helicobacter pylori
Allen, L. H., & Allgood, J. A. (2002) · Current Biology · doi:10.1016/s0960-9822(02)01216-2 · Cited by 42 · see citing works
Proteomic analysis reveals a role for protein kinase C-α in phagosome maturation
Hing, J. D. N. Y., Desjardins, M., & Descoteaux, A. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.05.054 · Cited by 41 · see citing works
Itoh, S., Suzuki, K., Nishihata, J., Iwasa, M., Oku, T., Nakajin, S., Nauseef, W. M., & Toyoshima, S. (2002) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.25.837 · Cited by 41 · see citing works
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Botelho, R. J., Harrison, R. E., Stone, J. C., Hancock, J. F., Philips, M. R., Jongstra‐Bilen, J., Mason, D. Y., Plumb, J. D., Gold, M. R., & Grinstein, S. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.009514 · Cited by 38 · see citing works
Self-citationDaniel, D., Dai, G., Singh, C. R., Lindsey, D. R., Smith, A. K., Dhandayuthapani, S., Hunter, R. L., & Jagannath, C. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.7.4688 · Cited by 38 · see citing works
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Martínez‐Augustin, O., Merlos, M., Zarzuelo, A., Suárez, M. D., & de Medina, F. S. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-490 · Cited by 32 · see citing works
Edlich, C., Stier, G., Simon, B., Sattler, M., & Muhle‐Goll, C. (2005) · Structure · doi:10.1016/j.str.2004.11.012 · Cited by 29 · see citing works
Todt, J. C., Hu, B., Punturieri, A., Sonstein, J., Polak, T., & Curtis, J. L. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202967200 · Cited by 28 · see citing works
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Cmarik, J. L., Hegamyer, G. A., Gerrard, B., Dean, M. C., & Colburn, N. H. (2000) · Genomics · doi:10.1006/geno.2000.6210 · Cited by 16 · see citing works
Lockhart, E. F., Dolcetti, F. J. C., Herrera, M. L., Pennini, J., Villar, M. F. Z., Salinas, G., Portiansky, E. L., Spittau, B., Lacunza, E., Hereñú, C. B., et al. (2022) · Molecular Neurobiology · doi:10.1007/s12035-022-02791-w · Cited by 13 · see citing works
Walker, M. G. (2003) · Genomics Proteomics & Bioinformatics · doi:10.1016/s1672-0229(03)01007-6 · Cited by 10 · see citing works
Higher expression of PLEK and LY86 as the potential biomarker of carotid atherosclerosis
Zhao, M., Liu, A., Mo, L., Wan, G., Lu, F., Chen, L., Fu, S., Chen, H., Fu, T., & Deng, H. (2023) · Medicine · doi:10.1097/md.0000000000034445 · Cited by 8 · see citing works
Protein expression changes induced in murine peritoneal macrophages by Group B Streptococcus
Susta, F., Chiasserini, D., Fettucciari, K., Orvietani, P. L., Quotadamo, F., Noce, R., Bartoli, A., Marconi, P., Corazzi, L., & Binaglia, L. (2010) · PROTEOMICS · doi:10.1002/pmic.200900642 · Cited by 8 · see citing works
Huang, L. T., Paredes, C. J., Papoutsakis, E. T., & Miller, W. M. (2007) · Physiological Genomics · doi:10.1152/physiolgenomics.00053.2007 · Cited by 8 · see citing works
Calcium Signaling during Phagocytosis
Melendez, A. J. (2008) · Springer eBooks · doi:10.1007/978-0-387-28669-3_9 · Cited by 5 · see citing works
Diversity in Phagocytic Signaling
García-García, E. (2008) · Springer eBooks · doi:10.1007/978-0-387-28669-3_1 · Cited by 4 · see citing works
Crystallization and preliminary diffraction analysis of truncated human pleckstrin
Jackson, S. G., Sugiman‐Marangos, S. N., Cheung, K. S. C., & Junop, M. S. (2011) · Acta Crystallographica Section F Structural Biology and Crystallization Communications · doi:10.1107/s174430911005092x · Cited by 4 · see citing works
[The mechanism of bactericidal activity in phagosomes of neutrophils].
Murav'ev, R. A., But, P. G., Фомина, В. А., & Vv, R. (2002) · Biology Bulletin · doi:10.1023/a:1016858817156 · Cited by 3 · see citing works
Tamura, K., Uchio‐Yamada, K., MANABE, N., Noto, T., Hirota, R., Unami, A., Matsumoto, M., & Miyamae, Y. (2013) · Nephron Experimental Nephrology · doi:10.1159/000354057 · Cited by 3 · see citing works
Dai, Y., Dunn, A. R., Hadad, N., Zhang, J.-G., Poirion, O., Korgan, A., White, B., Philip, V., Neuner, S., O’Connell, K. M. S., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.04.08.487664 · Cited by 2 · see citing works
Pisu, D., Sunny, P. S., Nelson, M. L., Junecko, B. A. F., Venugopalan, R., Rodgers, M., Borish, H. J., Maiello, P. A., Scanga, C. A., Lin, P. L., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.12.659348 · Cited by 2 · see citing works
Junop, M. S. (2022) · Annals of Experimental and Molecular Biology · doi:10.23880/aemb-16000111 · Cited by 1 · see citing works
Actions of cannabinoids on amoebae
Al-Hammadi, I. (2020) · Lancaster EPrints (Lancaster University) · doi:10.17635/lancaster/thesis/1108 · Cited by 1 · see citing works
Alshammery, S., Patel, S., Jones, H. F., Han, V. X., Gloss, B., Gold, W. A., & Dale, R. C. (2021) · Research Square · doi:10.21203/rs.3.rs-885680/v1
Upregulation of Trem2 expression occurs exclusively on microglial contact with plaquesPreprint
Wood, J. I., Wong, E., Joghee, R., Balbaa, A., Vitanova, K. S., VanSchoiack, A., Phelan, S.-L. J., Launchbury, F., Desai, S., Tripathi, T., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.26.477873
- Authors
- Brumell, J. H., Howard, J. C., Craig, K., Grinstein, S., Schreiber, A. D., & Tyers, M.
- Affiliations
- Mount Sinai Hospital (CA)
- Hospital for Sick Children (CA)
- University of Pennsylvania (US)
- Corresponding author
- Craig, Karen
- Tyers, Mike
- Venue
- The Journal of Immunology
- Publisher
- Oxford University Press (OUP)
- Published
- 1999-09
- Volume
- 163
- Issue
- 6
- Pages
- 3388-3395
- ISSN
- 0022-1767 (ISSN-L)
- 1550-6606
- OpenAlex
- W1514153354
- Cited by
- 54
- References
- 54
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 6 · 2021: 2 · 2020: 1 · 2018: 1 · 2015: 1 · 2013: 1
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Immunology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Medical Research Council Canada
- National Institutes of Health (AI-22193)
- Natural Sciences and Engineering Research Council of Canada
- Medical Research Council
- National Institute of Allergy and Infectious Diseases (AI-22193)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Phagocytosis and Immune Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- pleckstrin, protein kinase C, macrophages, phagosome, phagocytosis, FCGR2A, zymosan, LPS, interferon-gamma, actin, phagosome maturation, adaptor proteins
- MeSH terms
- Animals, Blood Platelets, Blood Proteins, Cell Line, Cricetinae, Immunoglobulin G, Interferon-gamma, Intracellular Membranes, Lipopolysaccharides, Macrophage Activation, Macrophages, Mice, Inbred BALB C, Mice, Inbred C57BL, Monocytes, Opsonin Proteins, Phagocytosis, Phagosomes, Phosphoproteins, Phosphorylation, Protein Kinase C, Substrate Specificity, Zymosan, CHO Cells, Cell Lineage, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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